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The map label for this gene is ndhJ [H]

Identifier: 33864157

GI number: 33864157

Start: 1996523

End: 1997080

Strand: Reverse

Name: ndhJ [H]

Synonym: PMT1891

Alternate gene names: 33864157

Gene position: 1997080-1996523 (Counterclockwise)

Preceding gene: 33864158

Following gene: 33864155

Centisome position: 82.84

GC content: 54.3

Gene sequence:

>558_bases
ATGAGCGAGACCCCTTCCACGCCCCCGGTGCCGGCGGGTCCGGAACCGGGACCTGTAAGCCGCTGGCTCAACCAGCAGGG
TTTTGAGCATCAGTTGCTTGATCCTGATCATCTTGGTGTGGAGGTGATTGGTGTGGAGTCGATGTTCCTGCAGGTGATCG
TTGCAGCTCTCAAGGCTGATGGTTTTGATTATCTTCAATGCCAGGGTGGCTATGACGAAGGCCCTGGCCAGCAGCTGGTC
TGCTTCTACCATCTCGTTGCCATGGCTGAAATGGTTGCAAAGATGGGGGTGGGAGATTCGTCGTCGGAAGCGGCCAAGGT
ACGTGAGGTACGCCTCAAGGTGTTTTTATCCCGTGAAGACATACCTTCGCTCCCATCGATCTACGGCTTGTTTCGCGGAG
CTGACTGGCAGGAGCGGGAAACCTTTGACATGTTCGGCATCAACTTTGAGGGACATCCCCATCCGAAAAGATTGCTAATG
CCTGAAGACTGGAAGGGCTGGCCACTGCGGAAAGATTATGTGCAGCCAGACTTTTATGAGATGCAGGATGCTTATTGA

Upstream 100 bases:

>100_bases
CTGCCTTGAAGCCTGGTGCTGGGCTACCAATGGCTGCGGAGTTGAACACCCCTGAAATTGATGTCAGCCCAGCTTCTCAG
TCTTCATCAACCTACGAGTC

Downstream 100 bases:

>100_bases
GCACTCTTTTTATACTTTCGATAGAAGCGCATCACCGCTAGGGCAAGGCTGCGTGGGTCATGGCGCAGTGTTGCGCGAGG
CCTTTTCCCTTGCAATGGAG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSETPSTPPVPAGPEPGPVSRWLNQQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLV
CFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDIPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLM
PEDWKGWPLRKDYVQPDFYEMQDAY

Sequences:

>Translated_185_residues
MSETPSTPPVPAGPEPGPVSRWLNQQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLV
CFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDIPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLM
PEDWKGWPLRKDYVQPDFYEMQDAY
>Mature_184_residues
SETPSTPPVPAGPEPGPVSRWLNQQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKADGFDYLQCQGGYDEGPGQQLVC
FYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSREDIPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMP
EDWKGWPLRKDYVQPDFYEMQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI145693162, Length=99, Percent_Identity=40.4040404040404, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010218
- InterPro:   IPR001268
- InterPro:   IPR020396
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa [H]

EC number: 1.6.5.3

Molecular weight: Translated: 20879; Mature: 20748

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETPSTPPVPAGPEPGPVSRWLNQQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKAD
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
GFDYLQCQGGYDEGPGQQLVCFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSRED
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCC
IPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMPEDWKGWPLRKDYVQPDFYE
CCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
MQDAY
HCCCC
>Mature Secondary Structure 
SETPSTPPVPAGPEPGPVSRWLNQQGFEHQLLDPDHLGVEVIGVESMFLQVIVAALKAD
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
GFDYLQCQGGYDEGPGQQLVCFYHLVAMAEMVAKMGVGDSSSEAAKVREVRLKVFLSRED
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCC
IPSLPSIYGLFRGADWQERETFDMFGINFEGHPHPKRLLMPEDWKGWPLRKDYVQPDFYE
CCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
MQDAY
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA