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The map label for this gene is fabI [H]

Identifier: 33864147

GI number: 33864147

Start: 1986408

End: 1987190

Strand: Reverse

Name: fabI [H]

Synonym: PMT1881

Alternate gene names: 33864147

Gene position: 1987190-1986408 (Counterclockwise)

Preceding gene: 33864148

Following gene: 33864146

Centisome position: 82.43

GC content: 54.15

Gene sequence:

>783_bases
ATGCTTCTCGATCTAAGCGGTAAGAAGATCCTCGTCACAGGTATCGCCAACAACCGTTCCATTGCCTGGGGCATCGCTCA
ACAGTTGAAAGCCGCCGGCGCTGAGCTGGGAATCACCTATTTGGCGGATGAAAGGGGCCGTTCGGAAGGCAAGGTGCGCG
ATCTCACTGCTCCTCTAGAACCCAGTTTGTTTTTGCCTTTGAATGTGCAGGACAGCAATCAGATCGAGGAGGTGTTTGCT
GTGATTGCCCAGCAGTGGGGCCAGTTGGATGGTTTGGTCCATTGTCTTGCCTTTGCAGGCAAGGAGGAATTGGTGGGTGA
TTACAGCGCCACCTCGGCCGAGGGATTCGCACGCGCCTTGGAGATCAGTGCCTACTCGCTGGCCCCGCTTTGTCATCACG
CCAAGCCTTTGTTTAGTGATGGTGCAGGGGTTGTGACGTTGACCTATCTCGGTGCAGATCGTGCCATTCCGAATTACAAC
GTGATGGGTGTTGCAAAGGCTGCTCTGGAGGCTTCAGTGCGTTATCTCTCTGCAGAACTTGGCCCTGATAAGCAGGTGCG
TGTGAACGCGATCAGTGCTGGCCCAATTCGCACCTTGGCTAGTTCTGCGATCGGCGGCATCCTCGAGATGATTCATAACG
TTGAAGAAAAGGCTCCCTTGCGTCGCACTGTGACCCAGATCGAAGTGGGCAATACAGCCGCGTTCTTGCTGAGCGATCTT
TCCAGTGGTATCTCCGGTCAGACGCTTTATGTGGATGCTGGTTACTGCATTAACGGAATGTGA

Upstream 100 bases:

>100_bases
ATCGAATAGAACTCTTGGCGACCTTATTCGGGGCATCCAGTGAAACTCGGCGTGGCATGGGAGAGAATCGCTGCCAATGT
TCCCTAGTGCGTTCGCTCTG

Downstream 100 bases:

>100_bases
TTTGACTGGCAGCATGACTTCATTTGGATGGGCATCATGACGTCGCTAAGACAGGGGGATGTTCATCGCGTCACCGCTGA
AACCGACGTGCTTGTTCGTG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLDLSGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLADERGRSEGKVRDLTAPLEPSLFLPLNVQDSNQIEEVFA
VIAQQWGQLDGLVHCLAFAGKEELVGDYSATSAEGFARALEISAYSLAPLCHHAKPLFSDGAGVVTLTYLGADRAIPNYN
VMGVAKAALEASVRYLSAELGPDKQVRVNAISAGPIRTLASSAIGGILEMIHNVEEKAPLRRTVTQIEVGNTAAFLLSDL
SSGISGQTLYVDAGYCINGM

Sequences:

>Translated_260_residues
MLLDLSGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLADERGRSEGKVRDLTAPLEPSLFLPLNVQDSNQIEEVFA
VIAQQWGQLDGLVHCLAFAGKEELVGDYSATSAEGFARALEISAYSLAPLCHHAKPLFSDGAGVVTLTYLGADRAIPNYN
VMGVAKAALEASVRYLSAELGPDKQVRVNAISAGPIRTLASSAIGGILEMIHNVEEKAPLRRTVTQIEVGNTAAFLLSDL
SSGISGQTLYVDAGYCINGM
>Mature_260_residues
MLLDLSGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLADERGRSEGKVRDLTAPLEPSLFLPLNVQDSNQIEEVFA
VIAQQWGQLDGLVHCLAFAGKEELVGDYSATSAEGFARALEISAYSLAPLCHHAKPLFSDGAGVVTLTYLGADRAIPNYN
VMGVAKAALEASVRYLSAELGPDKQVRVNAISAGPIRTLASSAIGGILEMIHNVEEKAPLRRTVTQIEVGNTAAFLLSDL
SSGISGQTLYVDAGYCINGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=49.0272373540856, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1789378, Length=245, Percent_Identity=25.3061224489796, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24643142, Length=266, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27472; Mature: 27472

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDLSGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLADERGRSEGKVRDLTAPLE
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEEEECCCCCCCCCEEECCCCCC
PSLFLPLNVQDSNQIEEVFAVIAQQWGQLDGLVHCLAFAGKEELVGDYSATSAEGFARAL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
EISAYSLAPLCHHAKPLFSDGAGVVTLTYLGADRAIPNYNVMGVAKAALEASVRYLSAEL
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GPDKQVRVNAISAGPIRTLASSAIGGILEMIHNVEEKAPLRRTVTQIEVGNTAAFLLSDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCHHHHHHHHH
SSGISGQTLYVDAGYCINGM
HCCCCCCEEEEECCEEECCC
>Mature Secondary Structure
MLLDLSGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLADERGRSEGKVRDLTAPLE
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEEEECCCCCCCCCEEECCCCCC
PSLFLPLNVQDSNQIEEVFAVIAQQWGQLDGLVHCLAFAGKEELVGDYSATSAEGFARAL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
EISAYSLAPLCHHAKPLFSDGAGVVTLTYLGADRAIPNYNVMGVAKAALEASVRYLSAEL
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GPDKQVRVNAISAGPIRTLASSAIGGILEMIHNVEEKAPLRRTVTQIEVGNTAAFLLSDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCHHHHHHHHH
SSGISGQTLYVDAGYCINGM
HCCCCCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]