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The map label for this gene is rne [H]

Identifier: 33864056

GI number: 33864056

Start: 1886902

End: 1888845

Strand: Reverse

Name: rne [H]

Synonym: PMT1789

Alternate gene names: 33864056

Gene position: 1888845-1886902 (Counterclockwise)

Preceding gene: 33864057

Following gene: 33864055

Centisome position: 78.35

GC content: 54.84

Gene sequence:

>1944_bases
ATGCCCCAGCAAATTGTCATCTCTGAGCAGCTGCGCATCGCAGCCTTGCTCACAGATGAGCGAGTCGATGAGTTGATCGT
TGCTCAGGGTCGATACCAGATCGGAGATGTCTACCTCGGCACGGTCGAGAACGTTCTTCCGGGAATTGATGCCGCTTTTG
TGAATATTGGGGAAAGTGAGAAAAATGGTTTTATCCATGTCACTGATCTGGGCCCTCTGCGTTTGAAGAAGGGTGCTGCA
GGGATCACCGAGCTGCTGGAGCCCCGTCAGCAGGTGTTGGTTCAGGTGATGAAGGAGCCCACAGGGTCTAAGGGACCAAG
GCTTACTGGCAACCTGGCGTTGCCTGGTCGCTACTTGGTGCTGCAGCCCCATGGCCAGGGGGTGAACATCTCTCGCCGGA
TTAATTCCGAAGGGGAACGCAATCGTTTGCGAGCACTTGGCGTACTAGTGAAGCCGCCGGGTGCTGGCCTGTTGATTCGC
ACCGAGGCCGATGGCATTAGTGAAGATCTGCTGATTGATGATCTGGAGGTTTTACTTAGGCAATGGGAAGCGATCCAGCA
GGCGGCAGACAATGCTCTACCACCGGTACTGCTCAATCGCGATGAAGATTTCATTCATCGCGTTTTGAGAGATCACGTCG
GTCCAGATCTTGTGAGAGTAGTGGTGAATGATGCCGCTGCCATTGAGCGTGTGAGCAGTTTCCTTGGCCAGGAAGGGCCA
AATGTATTAGTTGAGGCCCATAACGAATCCACCGAACTTCTTGAGCATTACCGAGTAAATGCTGCCATCCGCGATGCTCT
CAAGCCCAGGGTAGATCTGCCATCCGGTGGCTACGTGATCATTGAGCCGACGGAGGCACTCACTGTTATTGATGTCAATT
CAGGCTCGTTCACTCGTTCGGCCAATGCTCGTCAGACGGTGCTCTGGACCAATTGTGAGGCAGCTATTGAGATTGCCCGC
CAGCTGAAGCTTCGCAATATTGGCGGTGTGATCATCATCGACTTCATCGACATGGAGTCGCGCCGTGATCAGTTGCAGTT
GCTGGAGCATTTCACAGCAGCCGTTCGCGATGATGCTGCTCGTCCTCAGATTGCCCAGTTGACTGAACTGGGGCTGGTGG
AACTCACTCGTAAGCGTCAGGGGCAGAACATTTATGAATTGTTTGGCAGGGCTTGTCCTGCCTGTGGTGGTCTTGGCCAT
GTGGCGGTACTACCTGGCAAAGATCTGCTACAGCCACTTGCTATGGCGACTGGATTGGTGAGATCAGCAGCCTCTGCTCG
AGCTGAAGCGCAGGTGCCAGGTGAGGGCGGGAATGGTCGCCGTAGGCGTGGTGGGCGCATTCGTTCTGGTGCGGTTGAGA
TGCCTGCACATACGCCTCCTTCTGGTGTGGCGAATGGAGTTGAGTCAACCACGTCTGTTGAACCTGAGAGACAAGAGCCT
TCGGGCTCGTCTGAGGGTGTTATTCGTCGCCAGGAACCTGAATTGGTTGCTGTTCCAATGAATTCAGGGCAGGAGGAGGT
TTTTGCTTGGCTTGGATTAAACCCTTTGCTTTTGATTGAGCCACCTCCAGAGAATGACAACTTTCTGGTTCGCGTGGTTC
GGCCTGGAGAAGATGCTGAGTCTGTCCTTGAGGAGGCTCGTGAGCAGCTTTCAGCTAGTTCCAATCGGCGTCGGCGTCGG
GGACGAGGTGGTGCTCGAGGGGCCGGCCGTGGCGCTCCGGAAGCCAGTGTCGAAACCCCTTCATCCAATGGCACAGAGGT
CGTTGTCCAGCAAGCCAAGGTTGTCGCAAAGAATGAGCCTCTAAGTGTTCAAATCACTCCTCTTGAGGTTGATCATTCAT
CGAGTAGCGCTTCTGTAGAAACCGTTTCTTCAATTTCCGAGCCGCAGGAAAGTGAACCCGAGGATCCGCGCCGCCGCCGC
CGTCGCTCCTCAGCTGCAGGCTGA

Upstream 100 bases:

>100_bases
TTCAACGGCCTTTGGCTGTTAATTCGTTTTCCACCCGATGCATTGAGCCGGCAGGCCATTGATCGGTTCTGAGTCCAGCA
GGGCTCCCTATTTATTTTCT

Downstream 100 bases:

>100_bases
TATGGACCCTTCGGCGGAATCCATCGCCGGAGTTGATGAGGTGGGTCGGGGCTGCTGGTTTGGTCCTGTGTTTGCAGGAG
CGGTTGTCCTCACTGAAGTT

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAA
GITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLIR
TEADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGH
VAVLPGKDLLQPLAMATGLVRSAASARAEAQVPGEGGNGRRRRGGRIRSGAVEMPAHTPPSGVANGVESTTSVEPERQEP
SGSSEGVIRRQEPELVAVPMNSGQEEVFAWLGLNPLLLIEPPPENDNFLVRVVRPGEDAESVLEEAREQLSASSNRRRRR
GRGGARGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEPQESEPEDPRRRR
RRSSAAG

Sequences:

>Translated_647_residues
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAA
GITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLIR
TEADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGH
VAVLPGKDLLQPLAMATGLVRSAASARAEAQVPGEGGNGRRRRGGRIRSGAVEMPAHTPPSGVANGVESTTSVEPERQEP
SGSSEGVIRRQEPELVAVPMNSGQEEVFAWLGLNPLLLIEPPPENDNFLVRVVRPGEDAESVLEEAREQLSASSNRRRRR
GRGGARGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEPQESEPEDPRRRR
RRSSAAG
>Mature_646_residues
PQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESEKNGFIHVTDLGPLRLKKGAAG
ITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLVLQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLIRT
EADGISEDLLIDDLEVLLRQWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGPN
VLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRSANARQTVLWTNCEAAIEIARQ
LKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAARPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHV
AVLPGKDLLQPLAMATGLVRSAASARAEAQVPGEGGNGRRRRGGRIRSGAVEMPAHTPPSGVANGVESTTSVEPERQEPS
GSSEGVIRRQEPELVAVPMNSGQEEVFAWLGLNPLLLIEPPPENDNFLVRVVRPGEDAESVLEEAREQLSASSNRRRRRG
RGGARGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEPLSVQITPLEVDHSSSSASVETVSSISEPQESEPEDPRRRRR
RSSAAG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=404, Percent_Identity=36.3861386138614, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082235, Length=391, Percent_Identity=37.3401534526854, Blast_Score=217, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 70028; Mature: 69897

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESE
CCCCEEECCCCEEEEHHHHHHHHHHHHHCCCEEECCEEECCHHHHHCCCCEEEEECCCCC
KNGFIHVTDLGPLRLKKGAAGITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLV
CCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLIRTEADGISEDLLIDDLEVLLR
ECCCCCCCEEEECCCCCCHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRS
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEC
ANARQTVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAA
CCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
RPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHVAVLPGKDLLQPLAMATGLV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
RSAASARAEAQVPGEGGNGRRRRGGRIRSGAVEMPAHTPPSGVANGVESTTSVEPERQEP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCCCCCCCC
SGSSEGVIRRQEPELVAVPMNSGQEEVFAWLGLNPLLLIEPPPENDNFLVRVVRPGEDAE
CCCCCCCEEECCCCEEEEECCCCHHHHEEEECCCEEEEEECCCCCCCEEEEEECCCCCHH
SVLEEAREQLSASSNRRRRRGRGGARGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCC
LSVQITPLEVDHSSSSASVETVSSISEPQESEPEDPRRRRRRSSAAG
CEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
PQQIVISEQLRIAALLTDERVDELIVAQGRYQIGDVYLGTVENVLPGIDAAFVNIGESE
CCCEEECCCCEEEEHHHHHHHHHHHHHCCCEEECCEEECCHHHHHCCCCEEEEECCCCC
KNGFIHVTDLGPLRLKKGAAGITELLEPRQQVLVQVMKEPTGSKGPRLTGNLALPGRYLV
CCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPHGQGVNISRRINSEGERNRLRALGVLVKPPGAGLLIRTEADGISEDLLIDDLEVLLR
ECCCCCCCEEEECCCCCCHHHHHHHEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQAADNALPPVLLNRDEDFIHRVLRDHVGPDLVRVVVNDAAAIERVSSFLGQEGP
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NVLVEAHNESTELLEHYRVNAAIRDALKPRVDLPSGGYVIIEPTEALTVIDVNSGSFTRS
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEC
ANARQTVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMESRRDQLQLLEHFTAAVRDDAA
CCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
RPQIAQLTELGLVELTRKRQGQNIYELFGRACPACGGLGHVAVLPGKDLLQPLAMATGLV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
RSAASARAEAQVPGEGGNGRRRRGGRIRSGAVEMPAHTPPSGVANGVESTTSVEPERQEP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCCCCCCCC
SGSSEGVIRRQEPELVAVPMNSGQEEVFAWLGLNPLLLIEPPPENDNFLVRVVRPGEDAE
CCCCCCCEEECCCCEEEEECCCCHHHHEEEECCCEEEEEECCCCCCCEEEEEECCCCCHH
SVLEEAREQLSASSNRRRRRGRGGARGAGRGAPEASVETPSSNGTEVVVQQAKVVAKNEP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCC
LSVQITPLEVDHSSSSASVETVSSISEPQESEPEDPRRRRRRSSAAG
CEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]