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The map label for this gene is metK [H]

Identifier: 33863937

GI number: 33863937

Start: 1774215

End: 1775474

Strand: Direct

Name: metK [H]

Synonym: PMT1670

Alternate gene names: 33863937

Gene position: 1774215-1775474 (Clockwise)

Preceding gene: 33863936

Following gene: 33863938

Centisome position: 73.59

GC content: 55.56

Gene sequence:

>1260_bases
ATGAGCCGTTACGTCTTCACCTCTGAGTCCGTCACAGAAGGGCATCCCGACAAGATCTGTGATCAAGTGAGTGATGCCGT
TCTAGACGCCCTACTCGCTCAAGACCCCAGCAGCCGAGTTGCCTGTGAAACAGTTGTCAACACAGGGCTCTGCCTCATTA
CAGGCGAAGTGACCTCAAACGCCCAGGTTGATTTCATCCATCTTGTTAGGAACGTAATCCGCGAGATTGGTTATAGCGGT
GCTCGTGCAGGTGGGTTTGATGCCACGAGTTGCGCCGTGCTCGTTGCCCTGGATCAACAATCACCAGACATCGCCCAAGG
GGTTAATGAAGCCGACGACCATTCAGGTGATCCTCTTGACAAGGTGGGAGCAGGAGACCAAGGGATCATGTTTGGCTACG
CCTGCGACGAGACCCCTGAGCTGATGCCCCTACCCATCAGCCTCGCCCATCGCCTTGCCCGTCAACTCGCTGCAGTCCGT
CATGACGGGTCTCTGAGCTACTTGCTACCTGATGGGAAAACCCAAGTCAGTGTGGTCTACGAAAACGACCGTCCTGTCGC
GATCGACACGATTTTGATCTCCACTCAACACACCTCGGAGGTAGAGGGCATCTCAGATGAGAATGGAATCCGTGAGCGGA
TCACTCAAGACCTCTGGACCCATGTCGTAGAACCAGCGACTGCTGACCTCCCCCTCAAACCCAATCGTGAGGCGACCCGC
TTTTTAGTCAATCCAACCGGTAAGTTTGTGGTGGGAGGCCCCCAGGGGGATGCTGGCCTTACTGGCCGGAAGATCATCGT
AGATACCTACGGCGGCTATGCCCGCCATGGTGGTGGAGCCTTTTCCGGCAAAGACCCAACCAAAGTGGACCGCTCTGCTG
CCTACGCCGCACGATTTGTGGCCAAGTGTCTTGTAGCAGCAGGCCTCGCTCAACGTGCAGAGGTGCAACTCAGCTATGCC
ATTGGCGTGGCTAAACCCGTATCGATCCTAGTGGAATCTTTTGGCACCGGAAAAGTCACGAATGACGAACTCACAGCCTT
GGTGCAAGACCATTTTGACCTGCGACCTGGTGCCATCATCGAACAATTCAAGCTGCGTCAGCTGCCTCAGCAACGCGGAG
GACGGTTCTACCAAGACACTGCTGCCTATGGCCACTTCGGCCGCCCTGATCTAAAGCTTCCCTGGGAAGACGTTACTGAC
AAGGCCTCCACTCTGCTTCAAGCAGAGGCCCAACAACAAAAACAGGGAAGCAGCCTCTAG

Upstream 100 bases:

>100_bases
ACACTGACGAACTACCACCACCTCATCCACCACTGGAATGATCTAGCCGTCAAGTAGCCGTCAAGGCAGACCCTAAAATT
ACGCATAACTTCAGTTCTCC

Downstream 100 bases:

>100_bases
AAACCCCATGCCTAACTCGTCCCTGGCGCTCGGCATCGACCTTGGCACCAGCGGCGTACGGCTTGCCGTGCTCAACGAGC
ACGGCAAGCTGATCCACACA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNAQVDFIHLVRNVIREIGYSG
ARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAAVR
HDGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYA
IGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLKLPWEDVTD
KASTLLQAEAQQQKQGSSL

Sequences:

>Translated_419_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNAQVDFIHLVRNVIREIGYSG
ARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAAVR
HDGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYA
IGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLKLPWEDVTD
KASTLLQAEAQQQKQGSSL
>Mature_418_residues
SRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSNAQVDFIHLVRNVIREIGYSGA
RAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLDKVGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAAVRH
DGSLSYLLPDGKTQVSVVYENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKCLVAAGLAQRAEVQLSYAI
GVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAIIEQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLKLPWEDVTDK
ASTLLQAEAQQQKQGSSL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=54.1561712846348, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=54.4080604534005, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=408, Percent_Identity=56.3725490196078, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=53.1328320802005, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=53.0150753768844, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=399, Percent_Identity=53.3834586466165, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=53.6340852130326, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=47.9899497487437, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=371, Percent_Identity=48.7870619946092, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=51.5669515669516, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=324, Percent_Identity=50.6172839506173, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=101, Percent_Identity=56.4356435643564, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=75, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=397, Percent_Identity=52.3929471032746, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=52.1410579345088, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=51.0050251256281, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=50.5025125628141, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=50.5025125628141, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=49.6103896103896, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=54.0322580645161, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44972; Mature: 44841

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSN
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCC
AQVDFIHLVRNVIREIGYSGARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLD
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHH
KVGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAAVRHDGSLSYLLPDGKTQVSVVY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
ENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
ECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKCLVAAGLAQRAEVQLSYAIGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHH
EQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLKLPWEDVTDKASTLLQAEAQQQKQGSSL
HHHHHHHCHHHHCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRYVFTSESVTEGHPDKICDQVSDAVLDALLAQDPSSRVACETVVNTGLCLITGEVTSN
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCC
AQVDFIHLVRNVIREIGYSGARAGGFDATSCAVLVALDQQSPDIAQGVNEADDHSGDPLD
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHH
KVGAGDQGIMFGYACDETPELMPLPISLAHRLARQLAAVRHDGSLSYLLPDGKTQVSVVY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
ENDRPVAIDTILISTQHTSEVEGISDENGIRERITQDLWTHVVEPATADLPLKPNREATR
ECCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKCLVAAGLAQRAEVQLSYAIGVAKPVSILVESFGTGKVTNDELTALVQDHFDLRPGAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHH
EQFKLRQLPQQRGGRFYQDTAAYGHFGRPDLKLPWEDVTDKASTLLQAEAQQQKQGSSL
HHHHHHHCHHHHCCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA