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The map label for this gene is glgX [H]

Identifier: 33863664

GI number: 33863664

Start: 1482387

End: 1484501

Strand: Direct

Name: glgX [H]

Synonym: PMT1397

Alternate gene names: 33863664

Gene position: 1482387-1484501 (Clockwise)

Preceding gene: 33863659

Following gene: 33863665

Centisome position: 61.49

GC content: 55.74

Gene sequence:

>2115_bases
GTGCCCAATGCTTTCTGCCTCGCCGCAGACACAGCTGTAAGCAAGATGCATCTCGGCACGCCTTGGCCCCTGGGCAGCAC
GATCACAACACGAGGCGTCAATTTCTCTGTGGCCGCCCCAACTGCAAAGCGCGTGGAGCTGCTGCTGTTTGCCGATGCAA
ATGCCACGCAGCCAGAACACATCCTCAAGCTCCAGCCTTGCCATCGATCCGGCGACTACTGGCATGTTGAGGTGGAAGGA
TTGCAAACTGGCTGCTGTTATGGCTATCGGATCTTCGGCCCCCACAACCCTGGCAGCGATAGCTTTCACCCCTCCAAGGT
GCTGCTTGATCCCTGCACAAGAGCCATCAGTGGCTGGGACATCTATCAAAGAGAACAGGCCAAGGGATCGTCGCCCAATA
TCCAAGCCTGCCTAAAGGGAGTGGTGTGCGAACGAGAACATTTTGACTTCGCATCCCATCCACGCCCCAGGACCCCATGG
GACCGGAGCGTGATCTACGAACTGCATGTAGGGGGGTTCAGCAGTGGCCCGAAGTCAGAGGTAACACCTCAGCGCCAAGG
CACCTTCCTGGGACTCATTGACAAACTTCCCTATCTGCGAAAACTGGGTATCACGGCCATAGAGCTGCTACCTATTTTTG
CTTTCGATCCCTCTGATGCGCCAACGGGGCGGGACAACTACTGGGGCTACAGCCCTCTGAACTGGTTCACTCCCCATCCC
AAATATGTGCATGGTGACGATCCACTGCAGGCTCGCCAACAAGTCCGCGAGCTGGTTGCGGCCTGTCATGACGAGGGTAT
TGAAGTCATCCTTGACGTGGTCTACAACCACACCACAGAAGGCAGCATTGATGGCCCCACACTCAGCTGGCGAGGCTTCA
GCGATGCTCTCTATTACCACCAAAACGACAGTGGCGAATACCTGGATGTAAGTGGTTGCGGCAACAGCATCGCCGCCAAT
CGTCCAATCGTGAGGCAGCTGATTCTCGAGTCGATGCGTTGCTGGGCCATCGAACTAGGAGTCGATGGCTTCCGCTTCGA
TCTAGGGATCGCCCTGAGCCGAGGCGAAGGCCTAAAGCCGCTCGAACAACCTCCCCTATTTGAAGAGATTGAAGCTGATC
CAGAGCTCAGCGATTTAAAACTGTTTAGCGAACCATGGGATTGCGGTGGCCTCTATCGACTCAGCGATTTCCCAGCACAC
CGAATCGGAACATGGAATGGCCATTACCGAGATGACCTACGTGCCTTCTGGAAAGGAGACGAAGACAGCGCCTGGAGGAT
GGGACAACGCCTAAGCGGAAGTGCTGATCTCTACAAAGGTGAGCCAGCTCCGCTGGGACGTTCCCTGAACTTCATCACTG
CACACGATGGCTTCACACTCAATGACCTGGTGAGTTTCAACCGCAAACACAATCTCGCCAATGGAGAGTGCAACCGCGAC
GGTGAAAATCACAACAACAGCTGGAACAACGGCATTGAAGGCCCCTGCAGTGACCATGCCGTGCAGGCCCTCCGCCATCG
CCAGATGCGCAATCTGATCGCCACCCTGTTGCTTTCAAGAGGAGTGCCAATGCTTTTGATGGGAGATGAAGTGGGCCGCA
GCCAGGGGGGGAACAACAACAGCTGGTGTCAGGACAACCCGCTCGGCTGGATGCTCTGGAACCCTGAACAATGCGATATG
GATTTGCACCTCTTCGTCAGCAGGCTGCTCATGATTCGCCACCAACTTTCGGAACTCTTCTCTCCCGTCAATCACCCCCC
TGAGGAGATGCCCGTACGTCTGCAGCAGAGTCCAGACGATCTCTGGATGCAATGGCATGGTGTTGAGATCGACAAGCCCG
ATTGGGGCAGCTGGTCCCATACCATCTGCTACAGCCTGAATCGAGGCTCCAGCGGTGCGGTGATGTGGATGGGCTTCAAC
GCCTACAGCAAAGCCATGCACTTTGATCTGCCTGAACCGACTTCTGCGTGGCACCGCATCCTGGACACAGCACGTCCTAG
CCCTGATGACCTACCCGCAAGGCCTAATGCATGGACTTCAGAGGACATTGATCTGCAGAACCACAGCATGTTGATCTTGC
TGGCAGAGGAATACGCCTCAAAACTCAAGCTCTAA

Upstream 100 bases:

>100_bases
AAAACCCAGAGGGGCCCGCTCAGCTGCTCTGGAGGATTGCCCGACTCCAATGCTTGGGACAACGTCATGACATTGTGAAA
CGGGCTGAACTTCTTCGCTT

Downstream 100 bases:

>100_bases
AAGGCAAAATCAAAAAAGCGGGCGGCGGGAATCGAACCCGCGTCATCAGCTTGGAAGGCTGAGGTCTTACCACTACACAA
CGCCCGCACTAGTACAGCAG

Product: isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILKLQPCHRSGDYWHVEVEG
LQTGCCYGYRIFGPHNPGSDSFHPSKVLLDPCTRAISGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPW
DRSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRKLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSIDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAAN
RPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEQPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAH
RIGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGECNRD
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDM
DLHLFVSRLLMIRHQLSELFSPVNHPPEEMPVRLQQSPDDLWMQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFN
AYSKAMHFDLPEPTSAWHRILDTARPSPDDLPARPNAWTSEDIDLQNHSMLILLAEEYASKLKL

Sequences:

>Translated_704_residues
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILKLQPCHRSGDYWHVEVEG
LQTGCCYGYRIFGPHNPGSDSFHPSKVLLDPCTRAISGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPW
DRSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRKLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSIDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAAN
RPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEQPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAH
RIGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGECNRD
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDM
DLHLFVSRLLMIRHQLSELFSPVNHPPEEMPVRLQQSPDDLWMQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFN
AYSKAMHFDLPEPTSAWHRILDTARPSPDDLPARPNAWTSEDIDLQNHSMLILLAEEYASKLKL
>Mature_703_residues
PNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILKLQPCHRSGDYWHVEVEGL
QTGCCYGYRIFGPHNPGSDSFHPSKVLLDPCTRAISGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWD
RSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRKLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHPK
YVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSIDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAANR
PIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEQPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAHR
IGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGECNRDG
ENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDMD
LHLFVSRLLMIRHQLSELFSPVNHPPEEMPVRLQQSPDDLWMQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNA
YSKAMHFDLPEPTSAWHRILDTARPSPDDLPARPNAWTSEDIDLQNHSMLILLAEEYASKLKL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=577, Percent_Identity=39.6880415944541, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79171; Mature: 79040

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEH
CCCCEEEECCHHHHHEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCC
ILKLQPCHRSGDYWHVEVEGLQTGCCYGYRIFGPHNPGSDSFHPSKVLLDPCTRAISGWD
EEEECCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCC
IYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWDRSVIYELHVGGFSSGPKSE
HHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
VTPQRQGTFLGLIDKLPYLRKLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
CCCCCCCCCHHHHHHCHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSIDGPTLSWRGFSDALYYH
CEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEE
QNDSGEYLDVSGCGNSIAANRPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKP
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCC
LEQPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAHRIGTWNGHYRDDLRAFWKGD
CCCCCCHHHHCCCCCHHHHHEECCCCCCCCEEEECCCCCHHCCCCCCCHHHHHHHHHCCC
EDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGECNRD
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCC
SWCQDNPLGWMLWNPEQCDMDLHLFVSRLLMIRHQLSELFSPVNHPPEEMPVRLQQSPDD
CCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCC
LWMQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNAYSKAMHFDLPEPTSAWHRI
CEEEEECCEECCCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHEECCCCCHHHHHHHH
LDTARPSPDDLPARPNAWTSEDIDLQNHSMLILLAEEYASKLKL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCC
>Mature Secondary Structure 
PNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEH
CCCEEEECCHHHHHEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCC
ILKLQPCHRSGDYWHVEVEGLQTGCCYGYRIFGPHNPGSDSFHPSKVLLDPCTRAISGWD
EEEECCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCC
IYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWDRSVIYELHVGGFSSGPKSE
HHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
VTPQRQGTFLGLIDKLPYLRKLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
CCCCCCCCCHHHHHHCHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSIDGPTLSWRGFSDALYYH
CEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEE
QNDSGEYLDVSGCGNSIAANRPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKP
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCC
LEQPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAHRIGTWNGHYRDDLRAFWKGD
CCCCCCHHHHCCCCCHHHHHEECCCCCCCCEEEECCCCCHHCCCCCCCHHHHHHHHHCCC
EDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGECNRD
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCC
SWCQDNPLGWMLWNPEQCDMDLHLFVSRLLMIRHQLSELFSPVNHPPEEMPVRLQQSPDD
CCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCC
LWMQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNAYSKAMHFDLPEPTSAWHRI
CEEEEECCEECCCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHEECCCCCHHHHHHHH
LDTARPSPDDLPARPNAWTSEDIDLQNHSMLILLAEEYASKLKL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]