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The map label for this gene is hemL [H]

Identifier: 33863564

GI number: 33863564

Start: 1383376

End: 1384662

Strand: Direct

Name: hemL [H]

Synonym: PMT1296

Alternate gene names: 33863564

Gene position: 1383376-1384662 (Clockwise)

Preceding gene: 33863562

Following gene: 33863568

Centisome position: 57.38

GC content: 57.26

Gene sequence:

>1287_bases
TTGAACACCACCCGCTCCCAGGCGATTTTCAGCGCCGCCCAGCGCTTAATGCCTGGTGGCGTTAGTTCTCCAGTGCGAGC
ATTCCGCTCCGTAGGCGGTCAACCGATCGTGTTTGACCGCGTAAAGGGTGCCTACGCCTGGGACGTCGATGGCAATCGCT
TCATCGACTACATCGGCAGCTGGGGGCCCGCAATCTGCGGCCATGCCCACCCAGAAGTCATCGTCGCTCTACAAGATGCG
CTTGAAAAGGGCACAAGCTTTGGTGCCCCATGTGAGCTAGAGAATCAACTAGCCGAAATGGTGATCGACGCCGTGCCCAG
CGTCGAGATGGTCCGTTTTGTGAATAGCGGCACTGAGGCCTGCATGTCTGTACTGCGCCTCATGCGCGCATTCACTGGCC
GCGACAAGCTCATCAAATTCGAAGGCTGCTATCACGGCCACGCCGACATGTTTTTGGTGAAAGCTGGATCCGGGGTGGCA
ACTCTCGGTCTACCCGACTCACCAGGGGTGCCCCGCAGCACCACAAGTAACACCCTTACTGCGCCCTACAACGACCTCGA
GGCAGTCAAAGAGCTGTTTGCCGAGAATCCTGACGCAATCAGCGGGGTGATCCTTGAGCCGGTCGTAGGAAATGCCGGAT
TCATTACTCCAGAGCCTGGTTTCCTGGAAGGTTTACGAGAACTCACCCGTGAACACGGAGCCTTGCTTGTATTTGATGAA
GTCATGAGCGGTTTCCGCATCAGCTACGGAGGTGCTCAGGCCCGCTTTGGCGTCACGCCTGATCTAACCACCATGGGGAA
AGTGATCGGTGGTGGGCTACCCGTTGGTGCTTATGGCGGCAGAGCCGAGATCATGGAGATGGTGGCCCCCGCAGGCCCCA
TGTACCAAGCCGGCACGCTGAGTGGCAACCCGCTGGCCATGACGGCTGGCATCAAGACCCTTGAGCTACTTAAGCAAGAA
GGCACCTATGAGCGCCTAGAAAGCACAACGGAGCGCTTAATTAAGGGCATTCTCGAAGCAGCCAAAGCAGCAGAGGTTCC
CATTACGGGCAACAGCATCGGTGCGATGTTCGGCTTCTTCCTTTGCGAAGGCCCAGTACGGAATTTCGAAGATGCCAAAG
CAACCGATACCGAACTCTTCGGCAAGCTGCATCGGGCCATGCTCGAAAGGGGCATCTACCTAGCCCCAAGTGCCTTCGAA
GCAGGCTTCACATCCCTTGCCCATTCCGAAGCAGACATCGAAACCACCCTGAAGGCATTCCGCGAGAGTTTTGCAGCAGT
GGCATGA

Upstream 100 bases:

>100_bases
GATGGCCTGACACAGTTCATCCGCTTCTGACCTTTTTGGGTCAAGATGGCGAACGGTTCGTATCAGCCACTTGGCTTCGG
TCCCTGTGACAGCTTCAGCC

Downstream 100 bases:

>100_bases
ACAGACAACAAAAATAGAGAGCTGTTCTTACAGCTCTCCTGGGCAAATACAAACAAGCAGAAGCCAAGGTGCTACATCTA
GAACTAAAAGTTTAGAAGCA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTTRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIVALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLIKGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDTELFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA

Sequences:

>Translated_428_residues
MNTTRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIVALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLIKGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDTELFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA
>Mature_428_residues
MNTTRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIVALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLIKGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDTELFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=300, Percent_Identity=30.6666666666667, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI13994255, Length=327, Percent_Identity=26.605504587156, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI24119277, Length=368, Percent_Identity=27.7173913043478, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37574042, Length=324, Percent_Identity=26.5432098765432, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226442705, Length=323, Percent_Identity=26.0061919504644, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI284507298, Length=149, Percent_Identity=34.8993288590604, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI226442709, Length=292, Percent_Identity=26.3698630136986, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=54.5882352941177, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1789016, Length=362, Percent_Identity=34.2541436464088, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=34.2948717948718, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1789759, Length=342, Percent_Identity=30.1169590643275, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787560, Length=323, Percent_Identity=30.3405572755418, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI145693181, Length=320, Percent_Identity=29.6875, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786991, Length=369, Percent_Identity=24.390243902439, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=28.2392026578073, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=374, Percent_Identity=26.7379679144385, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=33.7837837837838, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=310, Percent_Identity=28.0645161290323, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=363, Percent_Identity=24.2424242424242, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357415, Length=300, Percent_Identity=26, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161085790, Length=432, Percent_Identity=24.3055555555556, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574759, Length=365, Percent_Identity=25.2054794520548, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45518; Mature: 45518

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGS
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHCC
WGPAICGHAHPEVIVALQDALEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTSNTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEE
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHH
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTL
HHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTAGIKTLELLKQEGTYERLESTTERLIKGILEAAKAAEVPITGNSIGAMFGFF
CCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LCEGPVRNFEDAKATDTELFGKLHRAMLERGIYLAPSAFEAGFTSLAHSEADIETTLKAF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHH
RESFAAVA
HHHHHHCC
>Mature Secondary Structure
MNTTRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGS
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHCC
WGPAICGHAHPEVIVALQDALEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTSNTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEE
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHH
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTL
HHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTAGIKTLELLKQEGTYERLESTTERLIKGILEAAKAAEVPITGNSIGAMFGFF
CCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LCEGPVRNFEDAKATDTELFGKLHRAMLERGIYLAPSAFEAGFTSLAHSEADIETTLKAF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHH
RESFAAVA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA