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The map label for this gene is xthA [H]

Identifier: 33863562

GI number: 33863562

Start: 1380858

End: 1381697

Strand: Direct

Name: xthA [H]

Synonym: PMT1294

Alternate gene names: 33863562

Gene position: 1380858-1381697 (Clockwise)

Preceding gene: 33863561

Following gene: 33863564

Centisome position: 57.28

GC content: 52.86

Gene sequence:

>840_bases
GTGCAGATCGCTACCTGGAACGTCAACTCAATTCGCACCCGCCTCGATCAGGTGCAAGCTTGGCTAAAGGATGCCCAACC
CGATCTGCTTTGCCTCCAGGAGACCAAAGTGGATGATCCGCTGTTCCCTCACGAGGTCTTTGAAGCTCAGGGCTATCAAG
TGCACTTTCACGGACAGAAGGCCTACAACGGCGTTGCAATCGTTAGCCGCCAACCTCTCGAAGATGTGCGCCGGGGCTTC
ACTGGTGAGCTCCCAGAGGATGCCGAAGCCTTGCAACTGGGAGAGCAAAAGCGCGTCATCAGCGCGCTCGTTAACAACAT
TCGTATCGTCAATGCCTACGTTCCCAACGGTTCAGCTCTGAAATCGGAGAAATATCCCTACAAACTCGAATGGCTGAGCT
GCCTAAACCGCTATCTGTGTGCACAAGCGAAACGGGATGAACCCCTCTGCCTCGTTGGGGATTTCAATATCGCCTTGGAA
GCAAGAGACATTCATCATCCCGAACTACTAACGGGAGGGATTATGGCTAGCGAGCTTGAAAGAGAGGCTCTTCTAAAGGT
TTTAGGAGACCGGCTCCACGACGTGTTCCGCGTGTTCGAACCAGATGCCAACCATTGGAGCTGGTGGGACTATCGAAGTG
GTGCCTGGGACCGTGATCAGGGTTGGCGTATTGATCACATCTACCTATGCGATGAGCTTCTCAGTCAGGCCAGAAGCTGT
GTGATTCACAAGCATCTAAGGGGCCATGAAAAGCCTAGTGATCATGCCCCGGTAAGCGTAGATCTCAACTGGACTCCAAC
TGATGACGATGAGGAGATGTCGGAACTGTTCAGCAATTGA

Upstream 100 bases:

>100_bases
GCGAAAGGGATTAAGACCTAAGGGGGCTTGGTTGATCAATGACGCATCCCCAAGTTCAGTAAGGCTAATGCTAATTGTCA
TGGTCAGATTTTCATGACCT

Downstream 100 bases:

>100_bases
AGGACATGATTCTTTAAGGCAATAGTCGCAGTCAAGGATTCATCTGCGGAGCAGTCAGAATGCCTTGGCTTCTGAAGGCA
TCTCAAGACCTTCTGAAGTC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQIATWNVNSIRTRLDQVQAWLKDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNAYVPNGSALKSEKYPYKLEWLSCLNRYLCAQAKRDEPLCLVGDFNIALE
ARDIHHPELLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVSVDLNWTPTDDDEEMSELFSN

Sequences:

>Translated_279_residues
MQIATWNVNSIRTRLDQVQAWLKDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNAYVPNGSALKSEKYPYKLEWLSCLNRYLCAQAKRDEPLCLVGDFNIALE
ARDIHHPELLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVSVDLNWTPTDDDEEMSELFSN
>Mature_279_residues
MQIATWNVNSIRTRLDQVQAWLKDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNAYVPNGSALKSEKYPYKLEWLSCLNRYLCAQAKRDEPLCLVGDFNIALE
ARDIHHPELLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVSVDLNWTPTDDDEEMSELFSN

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=37.4074074074074, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71989536, Length=272, Percent_Identity=26.1029411764706, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=27.6363636363636, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=277, Percent_Identity=27.7978339350181, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32137; Mature: 32137

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIATWNVNSIRTRLDQVQAWLKDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEECCCC
AYNGVAIVSRQPLEDVRRGFTGELPEDAEALQLGEQKRVISALVNNIRIVNAYVPNGSAL
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHCEEEEEEECCCCCCC
KSEKYPYKLEWLSCLNRYLCAQAKRDEPLCLVGDFNIALEARDIHHPELLTGGIMASELE
CCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCCHHHHCCHHHHHHH
REALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VIHKHLRGHEKPSDHAPVSVDLNWTPTDDDEEMSELFSN
HHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQIATWNVNSIRTRLDQVQAWLKDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEECCCC
AYNGVAIVSRQPLEDVRRGFTGELPEDAEALQLGEQKRVISALVNNIRIVNAYVPNGSAL
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHCEEEEEEECCCCCCC
KSEKYPYKLEWLSCLNRYLCAQAKRDEPLCLVGDFNIALEARDIHHPELLTGGIMASELE
CCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCCHHHHCCHHHHHHH
REALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VIHKHLRGHEKPSDHAPVSVDLNWTPTDDDEEMSELFSN
HHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]