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The map label for this gene is sigA [H]

Identifier: 33863540

GI number: 33863540

Start: 1359893

End: 1361278

Strand: Direct

Name: sigA [H]

Synonym: PMT1272

Alternate gene names: 33863540

Gene position: 1359893-1361278 (Clockwise)

Preceding gene: 33863538

Following gene: 33863541

Centisome position: 56.41

GC content: 51.01

Gene sequence:

>1386_bases
ATGGGTTCTGCTCCCTCATTCCCACTTTCCTCTTTCATTCCCTCCCCTGCCATGAGTCCTGCAGCCACCCAAGCAGCAGC
TGCAAAGGCCCAGCCAAAAGCCTCTGCAAAGACTGCCAAACCCCAAGTGGCCAAAGGCACGGCTAAAACCAAAGCCAAGA
AAGTCAAGGCAACAGCAGCTAAGGCAAAGACTGCTGCCAAGCCGGTTAGCTCTGCTGCAAAAGCGTCCAGCCCCAAAGCA
AAAAAAGCAAAAGCCACCCCTCCCCCTACTGTCAGCAAAAACCTTGATTTAACTGCTGACCAACTTCTCGCTACAGCAGC
GACAAGCGCCCCTAAGGCAAGCGCTGAAACTGAAAGCAGCCAAGCTGCTGCTAAAGCAACCAGCGAAGCAGACGCGAAGG
CCAGAGCTCTAGCCAATATCAAGATCGGTCCTAAAGGCGTCTACACCGAAGATTCCATCAGGGTTTATCTCCAGGAGATC
GGCCGTATCCGCCTGCTGCGTCCGGACGAGGAGATTGAACTAGCCCGCAAAATTGCTGACCTCCTGCATCTAGAGGAACT
GGCAGAGCAGTTCAATAGTGATCACGGTCACTACCCCAACAACAAGGAATGGGCTTCCCTTGTTGAAATGCCCAACATCA
AATTCCGCCGTCGCCTGATGCTGGGGCGCCGAGCCAAGGAAAAGATGGTGCAATCCAACCTGCGGCTGGTTGTTTCGATC
GCCAAAAAATACATGAATCGAGGCTTGTCCTTCCAGGATCTAATCCAAGAAGGCAGCCTTGGACTAATTCGTGCTGCCGA
AAAATTCGACCACGAGAAAGGGTACAAATTCTCCACCTATGCCACCTGGTGGATCCGTCAAGCCATCACCCGAGCAATTG
CGGATCAGAGCCGAACAATCCGTCTTCCTGTTCACCTCTACGAAACAATCTCACGCATCAAAAAAACAACCAAAGTGCTG
AGCCAGGAGTTTGGCCGTAAACCAACAGAAGAGGAAATTGCCGAAAGCATGGAGATGACAATCGAAAAACTTCGATTTAT
CGCAAAAAGTGCTCAACTACCCATCTCCCTCGAGACACCGATTGGCAAAGAGGAAGACTCCAGACTTGGTGATTTCATCG
AATCTGATACTGAGAATCCAGAACAAGATGTTGCCAAAAATCTCTTGCGAGAAGATCTAGAAGGTGTTCTCGCAACCCTC
AGCCCCCGCGAACGTGATGTTCTGCGTCTGCGCTATGGCCTTGACGATGGCCGCATGAAAACCCTCGAGGAAATTGGACA
GATCTTTGAGGTCACACGAGAGCGCATCCGTCAGATTGAAGCCAAAGCCCTACGCAAACTTCGCCACCCGAATCGCAATG
GTGTTTTAAAGGAATACATCAAGTAA

Upstream 100 bases:

>100_bases
GCTACTTCTAGCTCAGATAATCACTTGTTTACGAGGTTCTGATCTGATGCTTTGAGCCGTCGGTGCTGCAGTGGCCACTT
TTGCATGGTTTTGCATAAGC

Downstream 100 bases:

>100_bases
TCAATGTAAAAGTCAATCCAAAAAGGGGGCTTAAGCCTCCTTTTTCAGTGCAGGCGATCAAGACCTGTCAGCCACTGATA
TCTTTAATTCCTAATATCAA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MGSAPSFPLSSFIPSPAMSPAATQAAAAKAQPKASAKTAKPQVAKGTAKTKAKKVKATAAKAKTAAKPVSSAAKASSPKA
KKAKATPPPTVSKNLDLTADQLLATAATSAPKASAETESSQAAAKATSEADAKARALANIKIGPKGVYTEDSIRVYLQEI
GRIRLLRPDEEIELARKIADLLHLEELAEQFNSDHGHYPNNKEWASLVEMPNIKFRRRLMLGRRAKEKMVQSNLRLVVSI
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKVL
SQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDTENPEQDVAKNLLREDLEGVLATL
SPRERDVLRLRYGLDDGRMKTLEEIGQIFEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK

Sequences:

>Translated_461_residues
MGSAPSFPLSSFIPSPAMSPAATQAAAAKAQPKASAKTAKPQVAKGTAKTKAKKVKATAAKAKTAAKPVSSAAKASSPKA
KKAKATPPPTVSKNLDLTADQLLATAATSAPKASAETESSQAAAKATSEADAKARALANIKIGPKGVYTEDSIRVYLQEI
GRIRLLRPDEEIELARKIADLLHLEELAEQFNSDHGHYPNNKEWASLVEMPNIKFRRRLMLGRRAKEKMVQSNLRLVVSI
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKVL
SQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDTENPEQDVAKNLLREDLEGVLATL
SPRERDVLRLRYGLDDGRMKTLEEIGQIFEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
>Mature_460_residues
GSAPSFPLSSFIPSPAMSPAATQAAAAKAQPKASAKTAKPQVAKGTAKTKAKKVKATAAKAKTAAKPVSSAAKASSPKAK
KAKATPPPTVSKNLDLTADQLLATAATSAPKASAETESSQAAAKATSEADAKARALANIKIGPKGVYTEDSIRVYLQEIG
RIRLLRPDEEIELARKIADLLHLEELAEQFNSDHGHYPNNKEWASLVEMPNIKFRRRLMLGRRAKEKMVQSNLRLVVSIA
KKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKVLS
QEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDTENPEQDVAKNLLREDLEGVLATLS
PRERDVLRLRYGLDDGRMKTLEEIGQIFEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=59.0361445783133, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1789098, Length=238, Percent_Identity=42.8571428571429, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=30.0411522633745, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=31.0679611650485, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 51119; Mature: 50988

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSAPSFPLSSFIPSPAMSPAATQAAAAKAQPKASAKTAKPQVAKGTAKTKAKKVKATAA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
KAKTAAKPVSSAAKASSPKAKKAKATPPPTVSKNLDLTADQLLATAATSAPKASAETESS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
QAAAKATSEADAKARALANIKIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIAD
HHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
LLHLEELAEQFNSDHGHYPNNKEWASLVEMPNIKFRRRLMLGRRAKEKMVQSNLRLVVSI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEE
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETP
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGKEEDSRLGDFIESDTENPEQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMK
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
TLEEIGQIFEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
GSAPSFPLSSFIPSPAMSPAATQAAAAKAQPKASAKTAKPQVAKGTAKTKAKKVKATAA
CCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
KAKTAAKPVSSAAKASSPKAKKAKATPPPTVSKNLDLTADQLLATAATSAPKASAETESS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
QAAAKATSEADAKARALANIKIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIAD
HHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
LLHLEELAEQFNSDHGHYPNNKEWASLVEMPNIKFRRRLMLGRRAKEKMVQSNLRLVVSI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEE
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETP
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGKEEDSRLGDFIESDTENPEQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMK
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
TLEEIGQIFEVTRERIRQIEAKALRKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]