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The map label for this gene is isiB [H]

Identifier: 33863073

GI number: 33863073

Start: 877149

End: 877673

Strand: Direct

Name: isiB [H]

Synonym: PMT0801

Alternate gene names: 33863073

Gene position: 877149-877673 (Clockwise)

Preceding gene: 33863067

Following gene: 33863078

Centisome position: 36.38

GC content: 44.0

Gene sequence:

>525_bases
ATGGCTGTCGGTATCTACTTTGCAACAACAACAGGTAAAACTGAGGATATTGCTGATCGGCTTCATCCTCTCCTCAATGG
CACTAATACACCTCAGGATTTAGCTGATCTACAGGACTTAAGCCCGCTAAACGATTTAGATGCGATTATCTGCGGTATTC
CAACATGGAACACAGGTGCAGATAGTGAACGATCTGGTACAGCGTGGGATTCCATGCTCGAAGGCATCTCCAAGCTAAGC
CTTAGTGGTAAACAAGTCGCAATCTATGGGCTCGGAGACTCATCGACTTATACCGAAAACTTCTGTGATGCTATTGAAGA
GCTGCATCAATGCTTCAAAAATGCAGGAGCGACAATGGTGGGATATGTCTCAAAAGATGGTTATACATTTGAAGATTCAA
AGAGTTTAATAGGGGACAAGTTTTGTGGTCTACCCCTCGATGAAGACAGCGAATCAGACATGACGGATGATCGACTGATG
TCATGGGCAATTCAGCTCAAGCAAGAGATACCTGCGTTAAATTAA

Upstream 100 bases:

>100_bases
AACAATCAATAATTTGTATAAATAAGCACGGGATTGAGTTAATAATGGACGACCTGAATAAGCCTTGATGCATTAAGACT
GGTTTGCTTGTATTAAATCC

Downstream 100 bases:

>100_bases
TGATACTTACAAGATATCTCTATTAAACAAGAGATCACAATTGCAAACAACTCATCCACTCTTTTTTTTACATCACATCG
ATCGATTAATAGCCTGGCAT

Product: flavodoxin FldA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MAVGIYFATTTGKTEDIADRLHPLLNGTNTPQDLADLQDLSPLNDLDAIICGIPTWNTGADSERSGTAWDSMLEGISKLS
LSGKQVAIYGLGDSSTYTENFCDAIEELHQCFKNAGATMVGYVSKDGYTFEDSKSLIGDKFCGLPLDEDSESDMTDDRLM
SWAIQLKQEIPALN

Sequences:

>Translated_174_residues
MAVGIYFATTTGKTEDIADRLHPLLNGTNTPQDLADLQDLSPLNDLDAIICGIPTWNTGADSERSGTAWDSMLEGISKLS
LSGKQVAIYGLGDSSTYTENFCDAIEELHQCFKNAGATMVGYVSKDGYTFEDSKSLIGDKFCGLPLDEDSESDMTDDRLM
SWAIQLKQEIPALN
>Mature_173_residues
AVGIYFATTTGKTEDIADRLHPLLNGTNTPQDLADLQDLSPLNDLDAIICGIPTWNTGADSERSGTAWDSMLEGISKLSL
SGKQVAIYGLGDSSTYTENFCDAIEELHQCFKNAGATMVGYVSKDGYTFEDSKSLIGDKFCGLPLDEDSESDMTDDRLMS
WAIQLKQEIPALN

Specific function: Low-potential electron donor to a number of redox enzymes [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=177, Percent_Identity=35.5932203389831, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1789262, Length=174, Percent_Identity=36.2068965517241, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008254
- InterPro:   IPR001226
- InterPro:   IPR010086 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: NA

Molecular weight: Translated: 18886; Mature: 18755

Theoretical pI: Translated: 3.90; Mature: 3.90

Prosite motif: PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGIYFATTTGKTEDIADRLHPLLNGTNTPQDLADLQDLSPLNDLDAIICGIPTWNTGA
CEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
DSERSGTAWDSMLEGISKLSLSGKQVAIYGLGDSSTYTENFCDAIEELHQCFKNAGATMV
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
GYVSKDGYTFEDSKSLIGDKFCGLPLDEDSESDMTDDRLMSWAIQLKQEIPALN
EEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVGIYFATTTGKTEDIADRLHPLLNGTNTPQDLADLQDLSPLNDLDAIICGIPTWNTGA
EEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
DSERSGTAWDSMLEGISKLSLSGKQVAIYGLGDSSTYTENFCDAIEELHQCFKNAGATMV
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
GYVSKDGYTFEDSKSLIGDKFCGLPLDEDSESDMTDDRLMSWAIQLKQEIPALN
EEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1720613; 2119231; 10388575 [H]