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The map label for this gene is pheT

Identifier: 33863010

GI number: 33863010

Start: 804474

End: 806921

Strand: Reverse

Name: pheT

Synonym: PMT0738

Alternate gene names: 33863010

Gene position: 806921-804474 (Counterclockwise)

Preceding gene: 33863014

Following gene: 33863002

Centisome position: 33.47

GC content: 49.22

Gene sequence:

>2448_bases
ATGCGGGTTCCTCTCTCCTGGCTGCAGGATCTAGTACAGCTGAATGAGACCGTCGAGATCCTCGCCGAAGGCTTATCGAT
GGCTGGATTTGAGGTTGAGGCTATTGATGATTTAGCTAGTCATGCACAAGGCGTGGTCGTTGGGTTGGTGAAAGACCATC
AACCCCACCCCAATGCCGACAAACTCAGTGTCTGTCAGGTGGATGTCGGGTCGGGCGAAGGTCTTCAGATCGTCTGTGGG
GCTCCAAATGTTCGGATTGGTATACACGTACCTGTTGCCATGGTTGGAGCAACCCTCCCTGCTGTCGGAATCAAGATCAA
GGCCAGTGAGCTTCGGGGGGTTCCAAGTCAAGGAATGATCTGCTCACTCGCGGAATTAGGGCTGGAATCAAATGGTAATG
GAATCGCCATCCTTGAAGAGATCGCTGAAAACGTTCCAGACCTCGGCCAGCCTGTAGGCCCTCTGCTCGGACTCGATGAC
ACAGTGCTTGAGTTGGCAATCACAGCCAATCGCCCCGATGGAATGTCGATGGTTGGTATCGCAAGAGAAGTTGCTGCTTT
AACAGGCGCAAGCCTGCAGCTTCCGCAACTTGATATGGCACCAATCCATAAGTCTTTTGAGCCTGATTCCACCAGCTCTG
CGTCCATGCTGAAGGGTGGTCTTTATGGCTTAACCGCCTTAGAGAATGTGGATGGTGAATTAACTTCGCCAGCCTGGTTA
AAGCAACGCTTGGAGCGTTCAGGATGCAAATCAGTGAATGGTGTCGTCGATATTACAAATCTCGTGATGTTGGAGCAAGG
TCAGCCATTGCATGCTTTTGATATTGATGCTCTTGAAATGATCACAGGTCAAACAGTTTCCGCAGAATCTTTTGGCCTTC
GACAAGCAAGAAATAATGAACTTTTTAATGGACTAGATGGACATCAACTTCAACTTAACGAAAATTGCCAGATCGTCACC
TGCCATGACATTCCTATAGCGCTTGCGGGTGTTATGGGTAGTGCTGAAAGCGGTGTGTCTGCCAAGACAAGGCGAGTATG
GCTGGAATCCGCAATGTTTACCCCAACGGCCGTGAGAACTACGTGCCGAGCTGTTGGCTTAAGGACCGATGCAAGCAGTC
GCTTTGAGAAAGGGTTGCCTGTTGAAATGACTTTGGCATCAGCCCGTCGAGCTGTGACACTAATGGAGGAACATTTAGGT
ATTAAATCAAATGGATGTTGGGTATATGGTGAGTCCCCAAAATCAGCTGAGCCTGTAAAACTTCGCCGCGAAGCTATCCA
TCGCTTACTCGGACCAATAGGTGTTGAGAATGACAACAGATACCTAGAAGACGACATTATCGAGACTTCGCTGCTCGCTC
TCGGATGCGAGTTGTCTCCTTATAACGAAGGGTGGTTGGTCATAGTTCCACCATCACGACGTAGAGATCTCTCGCGAGAG
GTTGATTTGATCGAAGAGGTATCTCGCCTTGTTGGCTTTGATCGTTTTGAGGCCAACCTGCCAGATCCACTTGAACCTGG
TGGGCTAACGACTGCTCAGACAGCTGAACGCCTATTGAGGCAGATGTTGTGCGGAGCAGGATTGCAGGAAGTCACGACCT
TGTCGTTAGTGGGTGCTGATAGTGATGAACCACAACGAATTGCAATTAGCAACCCTTTATTGGCTGAGACCAGCCACCTG
CGTACCAATCTTTGGGAGGAGCATCTACGTATTTGCCAACGCAACCTCCAGTCATCGCAGCCTGGTTGTTGGTTATATGA
AATAGGCAATGTCTATACAGTCACTGACGAGCTCATCAACCAACGCGCTGTTCTTGGTGGCGTGATCTGTGCAGAGCGAA
GCTTTGAACGATGGAGTACAAGCGGCAAGATCAAGTCAATGACATATCACCAGGCACGCGGTCAGCTTTCCCAAGTCTTT
CAAGGGTTGAAGTTGGACATTAACGACCGTCCACTCAAGGACAATCATTCACTTCATCCTGGGCGGTCTGCAGAGTTATT
TGTCGAAGGCAAACTGCTCGGGGATTTTGGGCAGCTTCATCCAGCACTATCGGAACGTCTTGATCTACCTGAAGCAACCT
ATCTATTCGCTTTGGACTTGCAGTGCATCATCCAAGCTGCGACACGATCTAATCGATGGAACCCTACCTTCCGACCATTC
CCGACAGTTCCAGCAATGGAACTTGATTTAGCTGTGATCGTTTCAAAGGAATGTAGTTGTTCAGATTTGATCCAGGCCAT
CAGAAAAGCTGGTAAACCGCTTCTAGAGCATGTAGAGCTGATTGATCGTTTTGAGGGTGGACAGCTTGATCCTTACTCTT
GCAGTCAAGCGTTCAGGTTGCGCTATCGGAGTAAAGACAGGACTCTTAGTGAAGACAAGGTCAACCCGATTCACGAGAAG
GTACGTCAAGCACTTGTCAAGCAGTTTTCTGCAGAGCTTAGGAGCTAA

Upstream 100 bases:

>100_bases
GAAGCGTAGTGAAGAACTAAGATCCTACAGGGCAGCCTCACATGACATGACGCCGTCGTTCTCTTGCTTCGTAAGATCTT
TGGCTGCATCTAGCTGATCC

Downstream 100 bases:

>100_bases
TACGAGTTAACAGTGCCAGACATTCGATATGCATTGTTTGTGGAAAGAAATCAATGGGTTGTAATTCATCAATCTGATAG
GGTCCATTTGGTACGATCAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNADKLSVCQVDVGSGEGLQIVCG
APNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWL
KQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNELFNGLDGHQLQLNENCQIVT
CHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGESPKSAEPVKLRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNHSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPF
PTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDPYSCSQAFRLRYRSKDRTLSEDKVNPIHEK
VRQALVKQFSAELRS

Sequences:

>Translated_815_residues
MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNADKLSVCQVDVGSGEGLQIVCG
APNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWL
KQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNELFNGLDGHQLQLNENCQIVT
CHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGESPKSAEPVKLRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNHSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPF
PTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDPYSCSQAFRLRYRSKDRTLSEDKVNPIHEK
VRQALVKQFSAELRS
>Mature_815_residues
MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNADKLSVCQVDVGSGEGLQIVCG
APNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWL
KQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNELFNGLDGHQLQLNENCQIVT
CHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGESPKSAEPVKLRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNHSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPF
PTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDPYSCSQAFRLRYRSKDRTLSEDKVNPIHEK
VRQALVKQFSAELRS

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=834, Percent_Identity=32.2541966426858, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_PROMM (Q7V7K5)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894570.1
- ProteinModelPortal:   Q7V7K5
- SMR:   Q7V7K5
- STRING:   Q7V7K5
- GeneID:   1728750
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0738
- NMPDR:   fig|74547.1.peg.737
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   PMAR74547:PMT0738-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 88975; Mature: 88975

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KLSVCQVDVGSGEGLQIVCGAPNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMI
CEEEEEEECCCCCCEEEEECCCCEEEEEECHHHHHHCCCCCCCEEEEHHHHCCCCCCCHH
CSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDDTVLELAITANRPDGMSMVGI
HHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCCCHHCCCCHHHEEEEEECCCCCCCHHHHH
AREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWL
HHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCHHHH
KQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNE
HHHHHHCCCCCCCCCEEEEHEEEECCCCCCEEECCHHHHHHCCCCCCCHHHCHHHHCCCC
LFNGLDGHQLQLNENCQIVTCHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRT
CCCCCCCCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
TCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLGIKSNGCWVYGESPKSAEPVK
HHHHHCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHH
LRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGAD
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
SDEPQRIAISNPLLAETSHLRTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELIN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHH
QRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVFQGLKLDINDRPLKDNHSLHP
HHHHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
GRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPF
CCCCEEEEEEEEECCHHHHCHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCC
PTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDPYSCSQAFRL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RYRSKDRTLSEDKVNPIHEKVRQALVKQFSAELRS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KLSVCQVDVGSGEGLQIVCGAPNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMI
CEEEEEEECCCCCCEEEEECCCCEEEEEECHHHHHHCCCCCCCEEEEHHHHCCCCCCCHH
CSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDDTVLELAITANRPDGMSMVGI
HHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCCCHHCCCCHHHEEEEEECCCCCCCHHHHH
AREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWL
HHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCHHHH
KQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNE
HHHHHHCCCCCCCCCEEEEHEEEECCCCCCEEECCHHHHHHCCCCCCCHHHCHHHHCCCC
LFNGLDGHQLQLNENCQIVTCHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRT
CCCCCCCCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
TCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLGIKSNGCWVYGESPKSAEPVK
HHHHHCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHH
LRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGAD
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
SDEPQRIAISNPLLAETSHLRTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELIN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHH
QRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVFQGLKLDINDRPLKDNHSLHP
HHHHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
GRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPF
CCCCEEEEEEEEECCHHHHCHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCC
PTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDPYSCSQAFRL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RYRSKDRTLSEDKVNPIHEKVRQALVKQFSAELRS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642