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The map label for this gene is malE [C]

Identifier: 33862966

GI number: 33862966

Start: 748870

End: 750138

Strand: Reverse

Name: malE [C]

Synonym: PMT0694

Alternate gene names: 33862966

Gene position: 750138-748870 (Counterclockwise)

Preceding gene: 33862967

Following gene: 33862965

Centisome position: 31.11

GC content: 45.78

Gene sequence:

>1269_bases
ATGAAGAGAAGGATATTTACGAAGATAGCTCTTCTATTCTCTTTGAGCAGTGTCGCACTATTTGCAAGTTGGGCCCTGAG
TACTCGCCCTATTCAGATCAATATCTTGATGCCAGCTCCATTTGCTGAGTCAACAACTGATCTGGTACAACAATTCAACA
AAGATCATCATGGCAGGATCCAACTACGGGTGACTCGAGGTCCACTTGAAACAGAAGCCGTATCTGATTTAGCCATCAGC
AGTCTGCTTTTAGGTAAAAGTCCATTCGATGCACTTTTAATTGATGTAACTTGGTTGCCGAAATACGCCGCCGCAGGATG
GCTTATTCCCTTGGATCCATGGATCGACCAACAAAAGATTGATTCCATTGCTCCGGGGGCAATGCTTGGAAATAATTTCG
ACGGGAAACTGTATCGATGGCCGCTTGTGGCGGACATGGGATTGCTTTATTGGCGGACTGATTTGATGAATAAACCACCG
CGTACCCCTGAGGAACTTATTAAAGTTAGTCTTAAACTCCAAAAAGAAGGTCGTGTTGCTAATGGCTATGTCTGGCAAGG
CCGTCAATATGAAGGCCTAAGTTGTGTATTTCTAGAAGTTCTTGACGGATTTGGAGGGCAATGGCTTGAACCAGAAACCG
ATAATGTTGGCCTCGACAGCTCCGCAAGTCTTAAGGCTGCAAGTTGGTTGCGTGAACTGATTCTCAGTGGAGCAAGCCCC
AAAGCAGTGATCAACTATGCAGAAAATGAAGCTCTACAAGCGTTTAAGTCTGGAGATGTTGCGCTAATGCGTAACTGGCC
TTATGCCTGGGGAGAACTTCAGAAGCCTAACAGCGATGTTAGAGGCAATGTTGGCGTAACCACGATGGTTGCTTCCGCTG
GCAATCGATCCACATCAACCCTAGGCAGCTGGGGCTTCTCGATCCTCAAGGGCTCATCGAACCCTCAAGCAGCAGCAGAG
GCAATTGCCTTCCTCACTTCAACATCTGCACAAAAAACACTGTTCTTGAACGAAAGTTATACGCCAACCAAAGTTGAACT
ATTCAAAGACCAAGAGCTGCTCTCAAAGTCACAAATTCTTCCGGAGCTTGCTAAAGCCCTAGAAAGCACTGATGAACGTC
CATCAACTCCTCTTTATGCACAGATTAGTGATGTATTACAACGAAATCTTAGTTCGATTTTAACAGGCCAATCCAGTGTT
AGCGATGCAATGGCCAACGCCCAAGCAAACACCGAGAAGATTCTTATGGCGGCAAGAGAAACTAAATGA

Upstream 100 bases:

>100_bases
ACCATGTGATAACTACTGCTAGTGTCGACTCATACGCAGTCAAATATTGTGGAGAAGAGCAAATGAAAAGTTTGGTTGAT
AAAATAGAAGCAACGCAGCC

Downstream 100 bases:

>100_bases
TCCAATTGCTTCTATTGCCGGCAGCTCTATTAATTGCCTTGGTCTTTGGTATTCCTCTACTGCGTTATTCCTGGTTGAGC
TTCCATGCTGATTCAGTCCT

Product: sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKRRIFTKIALLFSLSSVALFASWALSTRPIQINILMPAPFAESTTDLVQQFNKDHHGRIQLRVTRGPLETEAVSDLAIS
SLLLGKSPFDALLIDVTWLPKYAAAGWLIPLDPWIDQQKIDSIAPGAMLGNNFDGKLYRWPLVADMGLLYWRTDLMNKPP
RTPEELIKVSLKLQKEGRVANGYVWQGRQYEGLSCVFLEVLDGFGGQWLEPETDNVGLDSSASLKAASWLRELILSGASP
KAVINYAENEALQAFKSGDVALMRNWPYAWGELQKPNSDVRGNVGVTTMVASAGNRSTSTLGSWGFSILKGSSNPQAAAE
AIAFLTSTSAQKTLFLNESYTPTKVELFKDQELLSKSQILPELAKALESTDERPSTPLYAQISDVLQRNLSSILTGQSSV
SDAMANAQANTEKILMAARETK

Sequences:

>Translated_422_residues
MKRRIFTKIALLFSLSSVALFASWALSTRPIQINILMPAPFAESTTDLVQQFNKDHHGRIQLRVTRGPLETEAVSDLAIS
SLLLGKSPFDALLIDVTWLPKYAAAGWLIPLDPWIDQQKIDSIAPGAMLGNNFDGKLYRWPLVADMGLLYWRTDLMNKPP
RTPEELIKVSLKLQKEGRVANGYVWQGRQYEGLSCVFLEVLDGFGGQWLEPETDNVGLDSSASLKAASWLRELILSGASP
KAVINYAENEALQAFKSGDVALMRNWPYAWGELQKPNSDVRGNVGVTTMVASAGNRSTSTLGSWGFSILKGSSNPQAAAE
AIAFLTSTSAQKTLFLNESYTPTKVELFKDQELLSKSQILPELAKALESTDERPSTPLYAQISDVLQRNLSSILTGQSSV
SDAMANAQANTEKILMAARETK
>Mature_422_residues
MKRRIFTKIALLFSLSSVALFASWALSTRPIQINILMPAPFAESTTDLVQQFNKDHHGRIQLRVTRGPLETEAVSDLAIS
SLLLGKSPFDALLIDVTWLPKYAAAGWLIPLDPWIDQQKIDSIAPGAMLGNNFDGKLYRWPLVADMGLLYWRTDLMNKPP
RTPEELIKVSLKLQKEGRVANGYVWQGRQYEGLSCVFLEVLDGFGGQWLEPETDNVGLDSSASLKAASWLRELILSGASP
KAVINYAENEALQAFKSGDVALMRNWPYAWGELQKPNSDVRGNVGVTTMVASAGNRSTSTLGSWGFSILKGSSNPQAAAE
AIAFLTSTSAQKTLFLNESYTPTKVELFKDQELLSKSQILPELAKALESTDERPSTPLYAQISDVLQRNLSSILTGQSSV
SDAMANAQANTEKILMAARETK

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46342; Mature: 46342

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIFTKIALLFSLSSVALFASWALSTRPIQINILMPAPFAESTTDLVQQFNKDHHGRI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEE
QLRVTRGPLETEAVSDLAISSLLLGKSPFDALLIDVTWLPKYAAAGWLIPLDPWIDQQKI
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHCCCEEECCCCCCHHHH
DSIAPGAMLGNNFDGKLYRWPLVADMGLLYWRTDLMNKPPRTPEELIKVSLKLQKEGRVA
HHCCCCHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCEE
NGYVWQGRQYEGLSCVFLEVLDGFGGQWLEPETDNVGLDSSASLKAASWLRELILSGASP
CCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KAVINYAENEALQAFKSGDVALMRNWPYAWGELQKPNSDVRGNVGVTTMVASAGNRSTST
HHEEECHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCH
LGSWGFSILKGSSNPQAAAEAIAFLTSTSAQKTLFLNESYTPTKVELFKDQELLSKSQIL
HHHCCHHEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHH
PELAKALESTDERPSTPLYAQISDVLQRNLSSILTGQSSVSDAMANAQANTEKILMAARE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHEEEECC
TK
CC
>Mature Secondary Structure
MKRRIFTKIALLFSLSSVALFASWALSTRPIQINILMPAPFAESTTDLVQQFNKDHHGRI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEE
QLRVTRGPLETEAVSDLAISSLLLGKSPFDALLIDVTWLPKYAAAGWLIPLDPWIDQQKI
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHCCCEEECCCCCCHHHH
DSIAPGAMLGNNFDGKLYRWPLVADMGLLYWRTDLMNKPPRTPEELIKVSLKLQKEGRVA
HHCCCCHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCEE
NGYVWQGRQYEGLSCVFLEVLDGFGGQWLEPETDNVGLDSSASLKAASWLRELILSGASP
CCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KAVINYAENEALQAFKSGDVALMRNWPYAWGELQKPNSDVRGNVGVTTMVASAGNRSTST
HHEEECHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCH
LGSWGFSILKGSSNPQAAAEAIAFLTSTSAQKTLFLNESYTPTKVELFKDQELLSKSQIL
HHHCCHHEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHH
PELAKALESTDERPSTPLYAQISDVLQRNLSSILTGQSSVSDAMANAQANTEKILMAARE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHEEEECC
TK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]