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The map label for this gene is dnaG [H]

Identifier: 33862914

GI number: 33862914

Start: 692462

End: 694516

Strand: Reverse

Name: dnaG [H]

Synonym: PMT0641

Alternate gene names: 33862914

Gene position: 694516-692462 (Counterclockwise)

Preceding gene: 33862915

Following gene: 33862913

Centisome position: 28.81

GC content: 54.06

Gene sequence:

>2055_bases
ATGCCCGTCCCCCGTCTTCACCCGCGCACGATCGAGGCCGTCAAGGAGCGTGCCGACATTGTTGATGTCGTTGGCGAACA
TGTGGTTCTAAAGAAAAAGGGCCGTGAATTCGTAGGGATCTGTCCGTTCCATGACGACAGCAAACCATCGATGACGGTCT
CGCCAGCGAAGCAGTTTTATTACTGCTTCTCTTGTGGAGCTGGTGGAAACTCGATCAAGTTCCTGATGGAGTTTCAGCGC
CAGAGTTTTGGTGATGTGGTGCTGGAGCTTGCTCGCAAGTATCAACTACCTGTTGAGACGGTCGAGGGGCCCCAGCAGGA
ACGACTACGTCAGCAGCTCTCTCGTCGAGACAAGCTTCATCGTGCGCTTGCATTGGCGGCCGGTTGGTTTCGTGGCCAGC
TGCGTACGCCAGTTGGTGCAAGTGCGCTGAATTACCTCACTGAGAAGCGGGGCCTTAGTGAAGTCACCTTGGAATCCTTC
GAGCTTGGCTATGCCCCTGAGCAATGGGATGGTTTACTCAAGCACCTCCAGCAGGTGGAAGGTTTGTCTGCTGAATTACT
GGAAGCCGCCGGGCTTGTTGTGGCGCGCAAGGGGGGAGGAGGTTTTTACGATCGCTTTCGTCATCGTGTGATGGTGCCGA
TCCACGACCGCCAGGGAAGGGTGATTGGTTTCGGTGGACGCAGTCTTGATGGCTCAGAGCCGAAGTACCTCAACTCTCCT
GAGACAGACGTGTTCGAGAAAGGCAAGCATTTGTTTGGACTGGATCGAGCGGCGAATGCCATCCGTAAGGATGACCGGGC
TGTGGTAGTCGAAGGATATTTCGATGTCATTGCATTGCATGCTGCAGGTGTCACCAATTCAGTGGCCTCCTTAGGCACTG
CTTTGAGTAGTCAGCAGATCACCCAGATCTGCCGTTGCAGCGAAGGGAAGCGGATCGTGCTTAATTTTGATGCTGATGGA
GCCGGTGTTCGTGCTGCGAACCGAGCCATTGGCGAAGTTGAGCAACTGGCTTTGCAAGGCCAGTTGGAATTACGAGTGCT
TCATTTGCCTTCTGGCAAAGACCCCGATGAATTTCTCAAAGATCATGGTGCTGGCGATTACAGGGCTCTATTAGATCAGT
CACCTCTTTGGCTTGATTGGCAGATTCAACAGGTGTTGGAGGGCCGTGATTTAAGTCAAGCGGATCAGTTTCAGCAAGCA
GTGAGTGGCCTGGTTGCATTGCTCGGCAAGTTGCCCCAGTCGGCCGTACGGACCCACTACCTGCAACAGGTTGCCGAACG
CTTGAGCGGTGGGCAGGGCCGGTTGGCCTTGCAGTTGGAGGAAGACCTGCGCCAACAAGTGAAAGGGCAGCGCTGGCATG
GCCGCTCAAGTCGCCATGAGCAACCTGGCGAGGCCAGTCAACGGGAGCGCAGCGAAGCGGAAATACTGAGGTTATATCTC
CATTGCTCAAATCATCGGGCTGCCATTCGCAGGGAGTTACGACAGCGAGAGCTGGAGGATTTTGCCTTACAACATCACCG
TTTGCTTTGGGCGGCGATCACTGATTTGGAGGAAACCAATCTTGGTGCAGGACGTTTAGAGGCGATCAGTCGCGGAACTG
ACTCTGGTCATGATTTGGAGGATATTGAGTTGCCACGGCTGCTTACCGATCAATTGCTCCTGGAGAACAGTGCTCTAGTC
GCTCGTCTTACCCCGTTATTAGAGCCTGGCGAGGTTCATTTGGCGGCTATGGCTAAGCCACTGCTTCAGCTCCGGGGCAC
AGCAGCGGCTCTAGAACGCCAGAAATCCCTCAAGCGTTGTCGTCATCTTCTTGAGGCCTGGGGTGGTCAGCGTCTTGAAA
CTCTTGAGCGTTGTATTGCTGCTTTGATTCAAGAAGAGCGACAGCAACCTACTGAATCAGTCGATATGGAAAAGCGTATC
GAAGCAATGTTTGAGGAGCTCAACAGCGATGCGTTGCGTTTCCAGGAGCTTTATTACAGCGAGCGCAAGCATATTGGTCA
CTTAGATCAGCAGCGGTGTGGTGGGTTCTCAGGCCAGGATGTCATTCCGGCTTAG

Upstream 100 bases:

>100_bases
TTGAGTTCTTATTGATCTCGGCCTTGCTTGTCGTATCGATTGATTTTGCTGATCATGTTCTCTTCTCGACCTCTCACGGC
GCAACCAGCAAAATAAGGTC

Downstream 100 bases:

>100_bases
GTCATCAGTCTGTGGATGCGCTTCACAAGCCAATCGGCGATATCAAAGAACTTGTTGTCTTGATTGACCATCACGATTCC
TGCTTGCATCTTCTCGCTGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQR
QSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGASALNYLTEKRGLSEVTLESF
ELGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADG
AGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQA
VSGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALV
ARLTPLLEPGEVHLAAMAKPLLQLRGTAAALERQKSLKRCRHLLEAWGGQRLETLERCIAALIQEERQQPTESVDMEKRI
EAMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA

Sequences:

>Translated_684_residues
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQR
QSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGASALNYLTEKRGLSEVTLESF
ELGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADG
AGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQA
VSGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALV
ARLTPLLEPGEVHLAAMAKPLLQLRGTAAALERQKSLKRCRHLLEAWGGQRLETLERCIAALIQEERQQPTESVDMEKRI
EAMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA
>Mature_683_residues
PVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFQRQ
SFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLHRALALAAGWFRGQLRTPVGASALNYLTEKRGLSEVTLESFE
LGYAPEQWDGLLKHLQQVEGLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSPE
TDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQITQICRCSEGKRIVLNFDADGA
GVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLKDHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAV
SGLVALLGKLPQSAVRTHYLQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYLH
CSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLEDIELPRLLTDQLLLENSALVA
RLTPLLEPGEVHLAAMAKPLLQLRGTAAALERQKSLKRCRHLLEAWGGQRLETLERCIAALIQEERQQPTESVDMEKRIE
AMFEELNSDALRFQELYYSERKHIGHLDQQRCGGFSGQDVIPA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=428, Percent_Identity=38.0841121495327, Blast_Score=283, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 76632; Mature: 76501

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCEEECCHHHEE
YCFSCGAGGNSIKFLMEFQRQSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHH
RALALAAGWFRGQLRTPVGASALNYLTEKRGLSEVTLESFELGYAPEQWDGLLKHLQQVE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
CCHHHHHHHCCEEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQI
CHHHHHCCCHHCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TQICRCSEGKRIVLNFDADGAGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLK
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
DHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAVSGLVALLGKLPQSAVRTHY
HCCCCHHHHHHCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
DIELPRLLTDQLLLENSALVARLTPLLEPGEVHLAAMAKPLLQLRGTAAALERQKSLKRC
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLLEAWGGQRLETLERCIAALIQEERQQPTESVDMEKRIEAMFEELNSDALRFQELYYS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
ERKHIGHLDQQRCGGFSGQDVIPA
HHHHCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PVPRLHPRTIEAVKERADIVDVVGEHVVLKKKGREFVGICPFHDDSKPSMTVSPAKQFY
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCEEECCHHHEE
YCFSCGAGGNSIKFLMEFQRQSFGDVVLELARKYQLPVETVEGPQQERLRQQLSRRDKLH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHH
RALALAAGWFRGQLRTPVGASALNYLTEKRGLSEVTLESFELGYAPEQWDGLLKHLQQVE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GLSAELLEAAGLVVARKGGGGFYDRFRHRVMVPIHDRQGRVIGFGGRSLDGSEPKYLNSP
CCHHHHHHHCCEEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETDVFEKGKHLFGLDRAANAIRKDDRAVVVEGYFDVIALHAAGVTNSVASLGTALSSQQI
CHHHHHCCCHHCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TQICRCSEGKRIVLNFDADGAGVRAANRAIGEVEQLALQGQLELRVLHLPSGKDPDEFLK
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
DHGAGDYRALLDQSPLWLDWQIQQVLEGRDLSQADQFQQAVSGLVALLGKLPQSAVRTHY
HCCCCHHHHHHCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LQQVAERLSGGQGRLALQLEEDLRQQVKGQRWHGRSSRHEQPGEASQRERSEAEILRLYL
HHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HCSNHRAAIRRELRQRELEDFALQHHRLLWAAITDLEETNLGAGRLEAISRGTDSGHDLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
DIELPRLLTDQLLLENSALVARLTPLLEPGEVHLAAMAKPLLQLRGTAAALERQKSLKRC
CCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLLEAWGGQRLETLERCIAALIQEERQQPTESVDMEKRIEAMFEELNSDALRFQELYYS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
ERKHIGHLDQQRCGGFSGQDVIPA
HHHHCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA