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The map label for this gene is gdhA [H]

Identifier: 33862896

GI number: 33862896

Start: 676622

End: 677977

Strand: Reverse

Name: gdhA [H]

Synonym: PMT0623

Alternate gene names: 33862896

Gene position: 677977-676622 (Counterclockwise)

Preceding gene: 33862897

Following gene: 33862894

Centisome position: 28.12

GC content: 43.29

Gene sequence:

>1356_bases
ATGTCTGATGCTACTAGCCATGAGATAGATCATTTCATGAAAGGTCTGATGAAGAGAAATCCTGGAGAGCCTGAGTTTCA
TCAAGCCGTCCATGAAGTTGTTGAAACTCTAATACCTTTTATTCTTTCCAACCGTGTTTACAAAGATGCACAGATTCTGG
AGCGGATGACGGAACCTGACCGCGTCGTTATCTTCAGAGTCTGCTGGGAAGATGATTCAGGCAATATCCGCACAAATCGA
GCATGGCGTGTACAGTTTAATAATTCCATTGGCCCTTATAAAGGTGGAATACGTTTCCATCCCAATGTCACCTTGAGTGT
CCTGAAATTTTTAGGTTTTGAACAGACCTTTAAAAACAGTCTTACTGGTCTGCCGATGGGTGGAGGGAAGGGTGGATCTA
ACTTTAATCCTAAAGGTAAAAGTGACCGGGAGATAATGCGATTTTGTCAATCTCTGATGATTGAACTTCATAGACATATT
GGTGAAGACACAGATGTACCTGCAGGTGATATCGGAGTCGGTTCTCGTGAAATTAGTTATATGTTCGGTCAGTACAAGCG
CCTTGAAAATCGGTTTAGTGGAATCTTGACTGGCAAGGGGCTGTCATTCGGTGGAAGTCTTGTTCGTACTGAAGCGACGG
GTTACGGATGTGCTTATTTCTGTGAAAATATGCTCAATCATATAGAGGACTCTCTCGTTGACAAAATATGTTCCATCTCC
GGTTCTGGAAACGTAGCCCTCTACGCAGTTGAAAAGGCCATTGAACTAGGAGCCAAAGTTGTTACTCTTTCTGACTCAGA
TGGTTTTGTACACGTCCCAAACGGCATTAATCTCGAGATGCTTGCGTTTATCAAAGAGCTAAAGACTGTTCGCCGTGGGC
GAATCCATGAATTTGCTAATCACTACAAAGGCGTAGATTTTTACGCAGGAAAGCGTCCATGGAATGTTCCCTGTGATATT
GCCTTCCCTTGTGCTACTCAAAATGAGATTAACCGTGCAGATTCACAGGAACTGATCAACAATGGTGTCAAAGCAGTTGT
TGAAGGTGCGAACATGCCAACCAATCTTGACGGCATTCATGAATTTCGTCATGCAAAAGTAATACTTGCTCCTGCCAAGG
CTGCAAATGCTGGGGGTGTCGCAGTCTCTGGCCTTGAGCAGAGTCAGAATGCATTGCGTCTATCTTGGAGTCGTGAAGAG
GTCGATCAACGCTTAAAGTCAATTATGTTGGAAATTCACTCCAAATGTGTTCAGCATAGTCATAAATACGAAGACTACAC
TGATTACATTACAGGAGCCAATATTGCAGGATTTACGAAAGTTGCTGATGCAATGCTGGCTTATGGAATTGTTTGA

Upstream 100 bases:

>100_bases
GTCATACTTTTAAGCTGATCTGGATAATATTCTGTATTAAAGTCACTTTAGGCGCTTGCCATGAAATCATACTAAATGAC
TTCTTGAGAAAGGACAGCTG

Downstream 100 bases:

>100_bases
TTTTATATCAGTGAATACATATGATTATGTTCGGCTGAGATCGAATCAAAAGGGACAAGCAAAGTTTCATGATTTTTACA
CTCTGTGTATCGGGTTGATG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSDATSHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDSGNIRTNR
AWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHI
GEDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFIKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDI
AFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREE
VDQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFTKVADAMLAYGIV

Sequences:

>Translated_451_residues
MSDATSHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDSGNIRTNR
AWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHI
GEDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFIKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDI
AFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREE
VDQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFTKVADAMLAYGIV
>Mature_450_residues
SDATSHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPDRVVIFRVCWEDDSGNIRTNRA
WRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNSLTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIG
EDTDVPAGDIGVGSREISYMFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSISG
SGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFIKELKTVRRGRIHEFANHYKGVDFYAGKRPWNVPCDIA
FPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIHEFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEV
DQRLKSIMLEIHSKCVQHSHKYEDYTDYITGANIAGFTKVADAMLAYGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=30.0291545189504, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=30.0291545189504, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=57.6233183856502, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17544676, Length=388, Percent_Identity=29.1237113402062, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=48.2300884955752, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.0176211453744, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45549226, Length=423, Percent_Identity=27.6595744680851, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI45553475, Length=423, Percent_Identity=27.6595744680851, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24649283, Length=322, Percent_Identity=31.9875776397516, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649460, Length=299, Percent_Identity=28.7625418060201, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 50040; Mature: 49908

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATSHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPD
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVVIFRVCWEDDSGNIRTNRAWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNS
EEEEEEEEEECCCCCEEECEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHC
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIGEDTDVPAGDIGVGSREISY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
HHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFIKELKTVRRGRIHEFAN
CCCCEEEEEHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HYKGVDFYAGKRPWNVPCDIAFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIH
HCCCCCEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
EFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEVDQRLKSIMLEIHSKCVQHS
HHHHCEEEEECCCCCCCCCEEEECHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
HKYEDYTDYITGANIAGFTKVADAMLAYGIV
CCCCHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDATSHEIDHFMKGLMKRNPGEPEFHQAVHEVVETLIPFILSNRVYKDAQILERMTEPD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVVIFRVCWEDDSGNIRTNRAWRVQFNNSIGPYKGGIRFHPNVTLSVLKFLGFEQTFKNS
EEEEEEEEEECCCCCEEECEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHC
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSLMIELHRHIGEDTDVPAGDIGVGSREISY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRLENRFSGILTGKGLSFGGSLVRTEATGYGCAYFCENMLNHIEDSLVDKICSIS
HHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSGNVALYAVEKAIELGAKVVTLSDSDGFVHVPNGINLEMLAFIKELKTVRRGRIHEFAN
CCCCEEEEEHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HYKGVDFYAGKRPWNVPCDIAFPCATQNEINRADSQELINNGVKAVVEGANMPTNLDGIH
HCCCCCEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
EFRHAKVILAPAKAANAGGVAVSGLEQSQNALRLSWSREEVDQRLKSIMLEIHSKCVQHS
HHHHCEEEEECCCCCCCCCEEEECHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
HKYEDYTDYITGANIAGFTKVADAMLAYGIV
CCCCHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]