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The map label for this gene is rpoD4 [H]

Identifier: 33862619

GI number: 33862619

Start: 394088

End: 395029

Strand: Direct

Name: rpoD4 [H]

Synonym: PMT0346

Alternate gene names: 33862619

Gene position: 394088-395029 (Clockwise)

Preceding gene: 33862618

Following gene: 33862621

Centisome position: 16.35

GC content: 50.53

Gene sequence:

>942_bases
ATGGTTTCAGCAGCGCCTAAATCAGCAGAAACACAGAGGCGTAGAAGTTCTGATCCTGTCAGCTGGTACCTCACAACGAT
TGGGCGTATACCTCTTCTTACCCCTGCTGAGGAGATTGAACTTGGCAATCAAGTTCAGACGATGATGAGTCTCACTCAAG
ACGGCTCAGTTGCGCCTGATGATAAGGAGTTTACGACACATCAGCGTCGGATGATTCGCATTGGTCGTCGTGCCAAAGAA
CGCATGATGAAGGCCAATCTTCGTCTGGTTGTGAGTGTTGCCAAGAAATATCAAGGCAAAGGACTGGAACTCCTCGATCT
CATTCAGGAGGGTTCACTTGGTTTAGAGCGTGCTGTTGAAAAGTTTGATCCAACCCGTGGCTATAAGTTTTCGACCTATG
CGTTTTGGTGGATTCGTCAGAGCATGACACGTGCGATTGCGTGCCAGTCGCGCACGATTCGCCTTCCTGTACATCTCAGT
GAAAGGCTGACCACAATTCGAAAGGTTTCTCTGGATTTGGCTCACAAGCTTGGAGCAATACCCAGTCGCTCCGAGATCGC
TGAAGCGATGGATATCCCTGTTGATGAACTCGACTCTTTACTGCGTCAGGCGCTAACAACCAGCAGCTTGGATGCGCCAG
TGAATGGTGAAGAAAGACGAAGTTTTCTTGGTGATCTGATCGCTGATTCCTCTCTTGGGGAACCTCTCGACAAGGTGGAG
CAGCGTATTCATCATGAGCAGCTCGGGCGTTGGCTCAGCCATCTCAGTGAGCAGGAGCAGCATGTCCTTAAGCTCCGTTT
TGGTCTTGAAACCCATGATCGCCATACCTTGGCTGAGATTGGTCGCTTGATGGAAGTCTCGCGTGAGCGTGTTCGTCAAG
TGGAATTAAAGGCCTTGCGCAAGCTGCGTAACCTCACGCGTAGGGTGCCCAACGAGATTTGA

Upstream 100 bases:

>100_bases
TTCGATTCCCCTGCATGAATCCGAAAGCTTTTTTAAAGCTGCAGGCTTTTGCTGAAATTTGTAGGAAAATTTCTTTCCAA
ATAAATCAGGAGCCGTTGAA

Downstream 100 bases:

>100_bases
AACTGTCTCCCTTATCTTGTTGGCTTTGCTTGCTTCCAGTACTTAGTGCTCAAGAGGGCTATTAATCTTCAGCCCAGATG
TAGCGAGTGAGTTCGTTGGG

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MVSAAPKSAETQRRRSSDPVSWYLTTIGRIPLLTPAEEIELGNQVQTMMSLTQDGSVAPDDKEFTTHQRRMIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLTTIRKVSLDLAHKLGAIPSRSEIAEAMDIPVDELDSLLRQALTTSSLDAPVNGEERRSFLGDLIADSSLGEPLDKVE
QRIHHEQLGRWLSHLSEQEQHVLKLRFGLETHDRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPNEI

Sequences:

>Translated_313_residues
MVSAAPKSAETQRRRSSDPVSWYLTTIGRIPLLTPAEEIELGNQVQTMMSLTQDGSVAPDDKEFTTHQRRMIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLTTIRKVSLDLAHKLGAIPSRSEIAEAMDIPVDELDSLLRQALTTSSLDAPVNGEERRSFLGDLIADSSLGEPLDKVE
QRIHHEQLGRWLSHLSEQEQHVLKLRFGLETHDRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPNEI
>Mature_313_residues
MVSAAPKSAETQRRRSSDPVSWYLTTIGRIPLLTPAEEIELGNQVQTMMSLTQDGSVAPDDKEFTTHQRRMIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLTTIRKVSLDLAHKLGAIPSRSEIAEAMDIPVDELDSLLRQALTTSSLDAPVNGEERRSFLGDLIADSSLGEPLDKVE
QRIHHEQLGRWLSHLSEQEQHVLKLRFGLETHDRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPNEI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=46.7811158798283, Blast_Score=220, Evalue=8e-59,
Organism=Escherichia coli, GI1789098, Length=292, Percent_Identity=40.4109589041096, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1789871, Length=240, Percent_Identity=28.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=28.6407766990291, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35815; Mature: 35815

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAAPKSAETQRRRSSDPVSWYLTTIGRIPLLTPAEEIELGNQVQTMMSLTQDGSVAPD
CCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DKEFTTHQRRMIRIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLTTIRKVSLDLAHKLGAI
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PSRSEIAEAMDIPVDELDSLLRQALTTSSLDAPVNGEERRSFLGDLIADSSLGEPLDKVE
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
QRIHHEQLGRWLSHLSEQEQHVLKLRFGLETHDRHTLAEIGRLMEVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRRVPNEI
HHHHHHHHCCCCC
>Mature Secondary Structure
MVSAAPKSAETQRRRSSDPVSWYLTTIGRIPLLTPAEEIELGNQVQTMMSLTQDGSVAPD
CCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DKEFTTHQRRMIRIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLTTIRKVSLDLAHKLGAI
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PSRSEIAEAMDIPVDELDSLLRQALTTSSLDAPVNGEERRSFLGDLIADSSLGEPLDKVE
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
QRIHHEQLGRWLSHLSEQEQHVLKLRFGLETHDRHTLAEIGRLMEVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRRVPNEI
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA