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The map label for this gene is miaB [H]

Identifier: 33862596

GI number: 33862596

Start: 369057

End: 370499

Strand: Reverse

Name: miaB [H]

Synonym: PMT0323

Alternate gene names: 33862596

Gene position: 370499-369057 (Counterclockwise)

Preceding gene: 33862597

Following gene: 33862595

Centisome position: 15.37

GC content: 56.41

Gene sequence:

>1443_bases
TTGTTTCGATCGTTCGCTTCGCCTCTTGCCTTGGTCGCCACCTCCGCCATCGCTAGTAATCCGGCAACAACCGCACAGCA
TCAAGGCAGTTTCTGGATTCAAACCTTCGGTTGCCAGATGAACAAGGCCGATTCAGAGCGCATGGCCGGAATTCTTGAGG
CGATGGGATACCACGAGGCGCCAGCAGAACTAGAAGCTGATCTCGTGCTCTACAACACCTGCACAATTCGCGATAACGCC
GAGCAGAAGGTTTACAGCTACCTAGGACGCCAGGCCCGCCGCAAACGAACACACCCCCATCTGAAGCTGGTCGTTGCCGG
TTGCGTAGCACAACAGGAAGGAGAAGCGCTACTGCGACGAATCCCTGAACTCGACCTTGTGATGGGGCCCCAACACGCCA
ACCGCCTTGAAGCCCTACTCACACAGGTGGACAATGGTCAGCAGGTTGTCGCCACAGATGACAACCACATTCTCGAGGAC
CTCACCACAGCTCGACGGGACAGCACAATCTGCGCCTGGGTCAATGTGATTTACGGCTGTAACGAGCGTTGTACCTATTG
CGTGGTGCCCTCCGTTCGGGGGAAGGAGCAATCAAGGTCGCCAGAGGCGATCCGACTGGAGATCGAGGGCCTGGCCGCCC
GTGGATTTCGGGAGATCACACTGCTTGGCCAGAACATTGATGCTTATGGACGCGATCTGCCCGGGATCACCCCTGAAGGC
CGGCGCCAAAACACACTCACCGATCTTCTCCATCACATTCATGACGTGGAAGGAATCGAGCGCATCCGCTTTGCGACAAG
CCATCCGCGCTACTTCACGGAACGATTGATCGAAGCTTGCTTTGATCTACCCAAAGTGTGCGAACACTTCCACATCCCTT
TCCAAAGCGGTGATAACGATGTGCTTAAGGCCATGGCTAGGGGTTACACAGTGGAGCGTTATCGACGCATCGTGAACCGA
ATCCGGGAGCTTATGCCCGATGCCGCTATCAGTACTGATGTGATCGTGGCCTTCCCAGGCGAAACCGACGCCCAGTTCCA
AAACACCTTGAACCTCCTCGAGGAAGTGGGTTTCGACCAGGTAAACACCGCGGCTTACTCCCCCCGTCCAAATACGCCTG
CAGCCACCTGGAGCAACCAACTCCCCGAAGCGGTGAAAGTGGAGCGACTCAAGCAACTCAATGCGCTGGTGGAACGCATA
GCCCTGCAGCGCAATAGCCGCTACTCCGGCAAGGTAGAACAGGTGCTAGCCGAAGGGATCAATCCCAAAAAACCACAACA
GCTGATGGGACGCACCCGCACCAACCGGCTCACATTCTTTGCGACCGAAGGACCCCAAGGTTGCCGCTATAGCCCAGGCG
ATCTCGTCGACATTCAGATCAATAGTGTGCGTGCTTTCTCCCTGAGCGGAACCCCTTGTGAGCAAACCAGAAGTCGGCAC
TGA

Upstream 100 bases:

>100_bases
CCAACGGCTGAGCAACTGAAATAAATAAATAGCGTTCACGACCTCATCTCCAAATCAACACAATCCATCAGGGCTCGACA
CACTGAAGGAGTTGATGAGA

Downstream 100 bases:

>100_bases
TAACTTTGGTCGCTAACACCCAAGGCCTTGCAGTATCTCCATGCCTTCCTCCCGCACCTGTGTAGGCGTGGTGTTCGGCG
GCGCGTCCGAGGAACATGCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFRSFASPLALVATSAIASNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLLEEVGFDQVNTAAYSPRPNTPAATWSNQLPEAVKVERLKQLNALVERI
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFATEGPQGCRYSPGDLVDIQINSVRAFSLSGTPCEQTRSRH

Sequences:

>Translated_480_residues
MFRSFASPLALVATSAIASNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLLEEVGFDQVNTAAYSPRPNTPAATWSNQLPEAVKVERLKQLNALVERI
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFATEGPQGCRYSPGDLVDIQINSVRAFSLSGTPCEQTRSRH
>Mature_480_residues
MFRSFASPLALVATSAIASNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLLEEVGFDQVNTAAYSPRPNTPAATWSNQLPEAVKVERLKQLNALVERI
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFATEGPQGCRYSPGDLVDIQINSVRAFSLSGTPCEQTRSRH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=32.4210526315789, Blast_Score=245, Evalue=8e-65,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=32.4210526315789, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI93277076, Length=377, Percent_Identity=32.8912466843501, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=39.3665158371041, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=26.1261261261261, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=422, Percent_Identity=34.3601895734597, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=380, Percent_Identity=26.0526315789474, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=31.9327731092437, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=28.0172413793103, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53841; Mature: 53841

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRSFASPLALVATSAIASNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEA
CCCCHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
PAELEADLVLYNTCTIRDNAEQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRR
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
IPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILEDLTTARRDSTICAWVNVIYGC
CCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
NERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
CCCCEEEEECCCCCCCCCCCCCEEEEEEECHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDND
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHH
VLKAMARGYTVERYRRIVNRIRELMPDAAISTDVIVAFPGETDAQFQNTLNLLEEVGFDQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCH
VNTAAYSPRPNTPAATWSNQLPEAVKVERLKQLNALVERIALQRNSRYSGKVEQVLAEGI
HCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
NPKKPQQLMGRTRTNRLTFFATEGPQGCRYSPGDLVDIQINSVRAFSLSGTPCEQTRSRH
CCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHCCCC
>Mature Secondary Structure
MFRSFASPLALVATSAIASNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEA
CCCCHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
PAELEADLVLYNTCTIRDNAEQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRR
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
IPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILEDLTTARRDSTICAWVNVIYGC
CCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
NERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
CCCCEEEEECCCCCCCCCCCCCEEEEEEECHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDND
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHH
VLKAMARGYTVERYRRIVNRIRELMPDAAISTDVIVAFPGETDAQFQNTLNLLEEVGFDQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCH
VNTAAYSPRPNTPAATWSNQLPEAVKVERLKQLNALVERIALQRNSRYSGKVEQVLAEGI
HCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
NPKKPQQLMGRTRTNRLTFFATEGPQGCRYSPGDLVDIQINSVRAFSLSGTPCEQTRSRH
CCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA