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The map label for this gene is ddl [H]

Identifier: 33862595

GI number: 33862595

Start: 367955

End: 369016

Strand: Reverse

Name: ddl [H]

Synonym: PMT0322

Alternate gene names: 33862595

Gene position: 369016-367955 (Counterclockwise)

Preceding gene: 33862596

Following gene: 33862594

Centisome position: 15.31

GC content: 55.74

Gene sequence:

>1062_bases
ATGCCTTCCTCCCGCACCTGTGTAGGCGTGGTGTTCGGCGGCGCGTCCGAGGAACATGCTGTTTCAATCCGATCAGCCAT
CACGGTGGTCGGAGCCCTACGCAGCGAAGTGAACAACAATCGCTTCGAGGTCATTGCGATTTACATCGACCAACGAGGCC
GCTGGTGGCCAGCTGGCGTTGCCGAAGCAGTGCTGAAGCAAGGTCATCCCGCCAAACCGGAACAACTGTCGACACCGCTT
GCCCCGCAGGGCTTTACCAAACTGCCTGAGGGGAGCGAAAGGGTACAGGTCTGGTATCCAGTTCTGCATGGTCCCAATGG
CGAAGACGGCACCGTGCAGGGCCTGTTCACCCTGATGGGACAACCTTTTGTGGGTTCAGGCGTACTCGGCTCTGCCCTCA
GCATGGACAAGTTGGCCATGAAAGCCGCTTTCGCTGCAGCAGGGTTACCACAGGTGCCTTACGCCGCCGTTGATGCCGCA
GATCTCCTTGAAACCGAATCGCGTCAAGGCGTCGCCAAACACTTGGAGGCCAAACTCAAATACCCATGTTTTGTCAAACC
CGCCAACCTGGGTTCTTCAGTCGGTATCAGCAAAGCTCAAAACCGCAACGAGCTGCTTATCGGTCTCGATAAGGCTGCCA
GCCTTGATCGGCGCATTGTGGTGGAGCAGGGAGTGAGCGCAAGAGAGCTTGAATGCGCTGTCCTAGGCAAGCGTGAACTG
CAAACCTCTGTCGTAGGGGAGATTTGCTTTGATGCGGACTGGTACGACTACGACACCAAATACAGCGAAAATTGCAGCCA
TACGCTCATTCCAGCGCCTCTACCAGAGGGAGTTGAAGCACAAATCCGCACACTGGCGTTACAAGCCTGTCGCTGCGTAG
CCGCCCATGGCATGGCCAGGGTCGACTTTTTTTACAACGCTGCACGCAATGAAATTTGGCTCAATGAGATCAACACCCTG
CCAGGCTTCACATCTCAGAGCATGTATCCAATGCTCTGGGAGGCTTCTGGTGTAACGCTCGAAGAGCTCGTGAGCCAACT
TGTGATCACAGCGAGAGAATAA

Upstream 100 bases:

>100_bases
AGTGTGCGTGCTTTCTCCCTGAGCGGAACCCCTTGTGAGCAAACCAGAAGTCGGCACTGATAACTTTGGTCGCTAACACC
CAAGGCCTTGCAGTATCTCC

Downstream 100 bases:

>100_bases
ACAAGTTCCAACTTGATGGCATTCCGGACTTCCATCTTTGACCTGCCAATGATTCACGGTCTGCTCTGGTTCCCCCTGCT
CCTGGTTTTTGTGCTTCTCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPSSRTCVGVVFGGASEEHAVSIRSAITVVGALRSEVNNNRFEVIAIYIDQRGRWWPAGVAEAVLKQGHPAKPEQLSTPL
APQGFTKLPEGSERVQVWYPVLHGPNGEDGTVQGLFTLMGQPFVGSGVLGSALSMDKLAMKAAFAAAGLPQVPYAAVDAA
DLLETESRQGVAKHLEAKLKYPCFVKPANLGSSVGISKAQNRNELLIGLDKAASLDRRIVVEQGVSARELECAVLGKREL
QTSVVGEICFDADWYDYDTKYSENCSHTLIPAPLPEGVEAQIRTLALQACRCVAAHGMARVDFFYNAARNEIWLNEINTL
PGFTSQSMYPMLWEASGVTLEELVSQLVITARE

Sequences:

>Translated_353_residues
MPSSRTCVGVVFGGASEEHAVSIRSAITVVGALRSEVNNNRFEVIAIYIDQRGRWWPAGVAEAVLKQGHPAKPEQLSTPL
APQGFTKLPEGSERVQVWYPVLHGPNGEDGTVQGLFTLMGQPFVGSGVLGSALSMDKLAMKAAFAAAGLPQVPYAAVDAA
DLLETESRQGVAKHLEAKLKYPCFVKPANLGSSVGISKAQNRNELLIGLDKAASLDRRIVVEQGVSARELECAVLGKREL
QTSVVGEICFDADWYDYDTKYSENCSHTLIPAPLPEGVEAQIRTLALQACRCVAAHGMARVDFFYNAARNEIWLNEINTL
PGFTSQSMYPMLWEASGVTLEELVSQLVITARE
>Mature_352_residues
PSSRTCVGVVFGGASEEHAVSIRSAITVVGALRSEVNNNRFEVIAIYIDQRGRWWPAGVAEAVLKQGHPAKPEQLSTPLA
PQGFTKLPEGSERVQVWYPVLHGPNGEDGTVQGLFTLMGQPFVGSGVLGSALSMDKLAMKAAFAAAGLPQVPYAAVDAAD
LLETESRQGVAKHLEAKLKYPCFVKPANLGSSVGISKAQNRNELLIGLDKAASLDRRIVVEQGVSARELECAVLGKRELQ
TSVVGEICFDADWYDYDTKYSENCSHTLIPAPLPEGVEAQIRTLALQACRCVAAHGMARVDFFYNAARNEIWLNEINTLP
GFTSQSMYPMLWEASGVTLEELVSQLVITARE

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=40.3314917127072, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1786280, Length=342, Percent_Identity=32.4561403508772, Blast_Score=157, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38174; Mature: 38043

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSRTCVGVVFGGASEEHAVSIRSAITVVGALRSEVNNNRFEVIAIYIDQRGRWWPAGV
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHH
AEAVLKQGHPAKPEQLSTPLAPQGFTKLPEGSERVQVWYPVLHGPNGEDGTVQGLFTLMG
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHC
QPFVGSGVLGSALSMDKLAMKAAFAAAGLPQVPYAAVDAADLLETESRQGVAKHLEAKLK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCC
YPCFVKPANLGSSVGISKAQNRNELLIGLDKAASLDRRIVVEQGVSARELECAVLGKREL
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCHHCCCCEEHHHCCCCHHHHHEEECCHHHH
QTSVVGEICFDADWYDYDTKYSENCSHTLIPAPLPEGVEAQIRTLALQACRCVAAHGMAR
HHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
VDFFYNAARNEIWLNEINTLPGFTSQSMYPMLWEASGVTLEELVSQLVITARE
HHHHHHHHCCEEEHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSSRTCVGVVFGGASEEHAVSIRSAITVVGALRSEVNNNRFEVIAIYIDQRGRWWPAGV
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHH
AEAVLKQGHPAKPEQLSTPLAPQGFTKLPEGSERVQVWYPVLHGPNGEDGTVQGLFTLMG
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHC
QPFVGSGVLGSALSMDKLAMKAAFAAAGLPQVPYAAVDAADLLETESRQGVAKHLEAKLK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCC
YPCFVKPANLGSSVGISKAQNRNELLIGLDKAASLDRRIVVEQGVSARELECAVLGKREL
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCHHCCCCEEHHHCCCCHHHHHEEECCHHHH
QTSVVGEICFDADWYDYDTKYSENCSHTLIPAPLPEGVEAQIRTLALQACRCVAAHGMAR
HHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
VDFFYNAARNEIWLNEINTLPGFTSQSMYPMLWEASGVTLEELVSQLVITARE
HHHHHHHHCCEEEHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA