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The map label for this gene is pbpF [H]

Identifier: 33862544

GI number: 33862544

Start: 310204

End: 312180

Strand: Reverse

Name: pbpF [H]

Synonym: PMT0271

Alternate gene names: 33862544

Gene position: 312180-310204 (Counterclockwise)

Preceding gene: 33862545

Following gene: 33862541

Centisome position: 12.95

GC content: 54.98

Gene sequence:

>1977_bases
GTGACTCGCAAGCGCCGTCACTGGATTGTTATTTCAGTTAGTGCTGTCGCCATCGGGTGTGTCGCAGCCTTGGCTGAAAG
GACTCTCACTCATGGCTTGGATTCGGTTCTGCCCGATGCCCGGCGGATAGCCAACTTCAACCGGCCAGGCACCATCACGC
TGCTCTCCACTGATGGGCAGGTGATCCAAAAGCTTGGTCCCGCGACCCGCGAGAAGGTTGCGCAAGACAAGATGCCTCTG
CTTGTGGAGCAAGCCTTCGTCGCGGCAGAAGACCGACGCTTTTACTACCACAATGGCTTGGATATCTGGGGTATCAGCCG
AGCCCTGGTGACAAACCTCCGCCAGGGTTCCGTGAGGGAAGGTGCCAGCACCATCACTCAGCAACTTGCTCGAACCGTAT
TCCTGAGTCAGGAACGAACCCTGCCGCGCAAACTCAAAGAAGCTGCTCTGGCCTACAAGCTGGAGCGACAGCTCAGCAAA
CAGCAAATCCTTGAGCAGTATCTGAATTATGTCTACTTGGGCTCAGGTGCTTACGGCGTAGCCGATGCAGCCTGGGTGTA
CTTCTCTAAATCCCCCAATCAACTCACACTGCCGGAAGCCGCTCTAATCGCTGGCATGCCCCCTGCTCCCTCAGTTTATT
CACCACTGGTCAACCAGAAAATTGCCCTAGAACGCCGCGCAATTGTCCTTAAACGAATGCAGCAGGCCGGATTTATCTCT
GCCAGAGAAGCCGATGCAGCTCGCGACAGCCCCCTCACTCTCAAGCCAGCGATACCGAAGTACTTCAACAGCAGCGCACC
ATTTTTCACCAGCTGGGTAGCCCAGCAACTCCCCAATCTGCTCACCCCAGAGCAACTAGAAGTGGGTGGACTCAAGATTC
ACACCAGCCTCAATCTGGCTTGGCAAAAGCAAGCCCAAGATGTGATCAGCAAATACGCCCCCGGGAACACAGAGGGTTCC
ATGGTGTCTATCGAACCAAGCACCGGTGAAGTGCGGGTGATGGTAGGAGGCAAAAACTTTAATACCAGCCAATTCAATAG
AGCAACACAGGCTCTGCGTTCACCTGGCTCCACCTTCAAGCTTTTTCCCTATGCAGCAGCCATCAACGCCGGCATCAAGC
CAGAGGACAAGGTTGTGGACGCGAAACGCTGCTGGAGAGGATATTGCCCAAAGAACTTCGGCAACAAATACCTGGGATCA
GTGGCACTCGCTGATGCCCTGAGATACTCCCTAAACACTGTTGCAGTCCAACTACTAGACAAAGTGGGCTTTGATGCCGT
GATCAGCATGGCCAATAATCTTGGCATCGGCACCACTCGCCCATTAGGCAAGTACTATCCGCTCGCTATTGGGGCCTACG
AGCAAACAGTACTCGACATGACGGCAGCCTATGCCGCAGTAGCCAACCGGGGCATTTACATGAAGCCCACGCCATTTGAA
GAGATCCGAGGCCCAGACGACGAGGTGATCTGGAACCACCAGTTAGAAGGCTACAAAGGTCGTCGAGCCCTCGATAGCGA
CGTAGCAGACACCATGAACTGGATGCTCCAGCGCGTTGTCAGCGGTGGCACTGGAGCTGCGGCATCCCTCGGTGACCGGC
CAGTAGCGGGCAAAACCGGCACATCGGAAGGTGCCCGTGACCTCTGGTTCATTGGCTCAATACCACAACTAACAACCGCC
GTATGGTTTGGCTACGACAACAACCGAAAGACATCTGGCGGCAGTGGTGATGCTGCATGGGCCTGGAAGCAGTTCATGAC
CAAGATCCAAGACAATTTTGAGGTACAACCCTTCCCACCAAAACCAGTTATGAACCGCACCTTTAGGCCACCAGGCAAAA
CGAGGAGCAAACCAAAGAACGGCAGCGAGGATGCTGCCAAAGACCAAGATCAAACCCAGGGCCCTCCACCCAGATACATC
GCACCTCCAGGGGGACCGCCGCTCAATGAAAACTTCGAGCCAATGCCTATTCCCTGA

Upstream 100 bases:

>100_bases
CCTTTGATGTTAGATATCAGACCAGTGCTCAACCGTTTCTAATCCTTGGCATGCTGGTTACTGCTCTAGCCATAGGCCAT
AGCCCATTAACACAGGGGAT

Downstream 100 bases:

>100_bases
TCAGGCTCAGCTGAGATCCAACACGGCTGAATAAAAACCATCACCACCATGCTCAGGATCAGGCCATAGTTGCTGTTCCT
GAGACAAGCTCAATTCAGGA

Product: penicillin binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MTRKRRHWIVISVSAVAIGCVAALAERTLTHGLDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVAQDKMPL
LVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSK
QQILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
AREADAARDSPLTLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQDVISKYAPGNTEGS
MVSIEPSTGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGS
VALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
EIRGPDDEVIWNHQLEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTA
VWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYI
APPGGPPLNENFEPMPIP

Sequences:

>Translated_658_residues
MTRKRRHWIVISVSAVAIGCVAALAERTLTHGLDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVAQDKMPL
LVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSK
QQILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
AREADAARDSPLTLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQDVISKYAPGNTEGS
MVSIEPSTGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGS
VALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
EIRGPDDEVIWNHQLEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTA
VWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYI
APPGGPPLNENFEPMPIP
>Mature_657_residues
TRKRRHWIVISVSAVAIGCVAALAERTLTHGLDSVLPDARRIANFNRPGTITLLSTDGQVIQKLGPATREKVAQDKMPLL
VEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVREGASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQ
QILEQYLNYVYLGSGAYGVADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFISA
READAARDSPLTLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLAWQKQAQDVISKYAPGNTEGSM
VSIEPSTGEVRVMVGGKNFNTSQFNRATQALRSPGSTFKLFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSV
ALADALRYSLNTVAVQLLDKVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFEE
IRGPDDEVIWNHQLEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTGTSEGARDLWFIGSIPQLTTAV
WFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPPKPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIA
PPGGPPLNENFEPMPIP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=535, Percent_Identity=32.5233644859813, Blast_Score=211, Evalue=2e-55,
Organism=Escherichia coli, GI87082258, Length=304, Percent_Identity=38.8157894736842, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788867, Length=595, Percent_Identity=29.0756302521008, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=38.2352941176471, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 72169; Mature: 72038

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKRRHWIVISVSAVAIGCVAALAERTLTHGLDSVLPDARRIANFNRPGTITLLSTDGQ
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCHH
VIQKLGPATREKVAQDKMPLLVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVRE
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHCCCHHH
GASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQQILEQYLNYVYLGSGAYGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCC
ADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
CCEEEEEEECCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AREADAARDSPLTLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLA
CHHCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHH
WQKQAQDVISKYAPGNTEGSMVSIEPSTGEVRVMVGGKNFNTSQFNRATQALRSPGSTFK
HHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
LFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSVALADALRYSLNTVAVQLLD
EEEHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
HHCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEECCCCHH
EIRGPDDEVIWNHQLEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTG
HHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
TSEGARDLWFIGSIPQLTTAVWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPP
CCCCCCCEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
KPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIAPPGGPPLNENFEPMPIP
CHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRKRRHWIVISVSAVAIGCVAALAERTLTHGLDSVLPDARRIANFNRPGTITLLSTDGQ
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCHH
VIQKLGPATREKVAQDKMPLLVEQAFVAAEDRRFYYHNGLDIWGISRALVTNLRQGSVRE
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHCCCHHH
GASTITQQLARTVFLSQERTLPRKLKEAALAYKLERQLSKQQILEQYLNYVYLGSGAYGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCC
ADAAWVYFSKSPNQLTLPEAALIAGMPPAPSVYSPLVNQKIALERRAIVLKRMQQAGFIS
CCEEEEEEECCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AREADAARDSPLTLKPAIPKYFNSSAPFFTSWVAQQLPNLLTPEQLEVGGLKIHTSLNLA
CHHCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHH
WQKQAQDVISKYAPGNTEGSMVSIEPSTGEVRVMVGGKNFNTSQFNRATQALRSPGSTFK
HHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
LFPYAAAINAGIKPEDKVVDAKRCWRGYCPKNFGNKYLGSVALADALRYSLNTVAVQLLD
EEEHHHHHCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGFDAVISMANNLGIGTTRPLGKYYPLAIGAYEQTVLDMTAAYAAVANRGIYMKPTPFE
HHCHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCEEECCCCHH
EIRGPDDEVIWNHQLEGYKGRRALDSDVADTMNWMLQRVVSGGTGAAASLGDRPVAGKTG
HHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
TSEGARDLWFIGSIPQLTTAVWFGYDNNRKTSGGSGDAAWAWKQFMTKIQDNFEVQPFPP
CCCCCCCEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
KPVMNRTFRPPGKTRSKPKNGSEDAAKDQDQTQGPPPRYIAPPGGPPLNENFEPMPIP
CHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]