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The map label for this gene is trmH [H]

Identifier: 33862541

GI number: 33862541

Start: 307753

End: 308490

Strand: Reverse

Name: trmH [H]

Synonym: PMT0268

Alternate gene names: 33862541

Gene position: 308490-307753 (Counterclockwise)

Preceding gene: 33862544

Following gene: 33862536

Centisome position: 12.8

GC content: 55.15

Gene sequence:

>738_bases
ATGAGGGACACGGCATCCCATACCCTCAAGTCAAAAGACGCTTGGCCCATGCCTCTGCTGCCTCGCCGATTTGATCGTCT
CAAGGCTGTTCTGGATTGCCGAATGGCAGATCTGACGGTTCTGCTTGAGCACGTAGAAAAACCCCACAACCTCTCAGCCA
TTCTGCGCAGCTGCGATGCGGTGGGTGTCCTGGAAGCCCATGCAGTGAACTATTCAGGACGCCTGCCCACCTTCAACAGT
ACCGCTCAGGGAAGCCAAAAGTGGATACCGCTCAAAGAGCACAGCAGCATCGAAGTTGCCGTCAAGGCCATCAAGGACAG
TGGCTTTCGCCTTTATGGAACCCATCTAGGTGGAAACGCACGCGACTACCGAGACTGCGACTTCTGCGAACCTACAGCCT
TCGTCCTGGGAGCTGAGAAATGGGGCCTTAGTAAGCTGGCGACGCCGTTGATGGATGAAGGGATCTTCATCCCTATGAGC
GGCATGGTGCAGTCGTTGAATGTTTCGGTAGCAGCAGCAACCTTGCTCTTTGAGGCCCTACGTCAACGACGGCTGGCTGG
TATCGCACCTCAAGCAGGCGAAGGCCTAGAACCAGCGCTTTATCAACAGCGCCTATTTGAGTGGGCCTATCCAGAAGTTG
CAGCATGGTGCCAGCAGGAAGGCAAGTCATATCCTGCCCTTAACGATCAAGGCGAAATCCAAGGAAGCCTCCCCCGCAAC
GTGAGATTGCGCTGTTAA

Upstream 100 bases:

>100_bases
CAAAGGTAATCGACGCATAGCCCAACCCACCTGACGGGCACATCCATAGGCACCGATCATCTCGGCAATCTGACCATAGG
TCGCTAACTGGCCGAAAGGG

Downstream 100 bases:

>100_bases
GCAACCAGCCAACACTCAAAATGGCATCTGCACATCTTTGCTTGCATGCGATCAATTGATCCCAAGTTGTTTTTGACGTG
CCGCGGGAAGACGCAAGCCA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRDTASHTLKSKDAWPMPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNS
TAQGSQKWIPLKEHSSIEVAVKAIKDSGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSKLATPLMDEGIFIPMS
GMVQSLNVSVAAATLLFEALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGKSYPALNDQGEIQGSLPRN
VRLRC

Sequences:

>Translated_245_residues
MRDTASHTLKSKDAWPMPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNS
TAQGSQKWIPLKEHSSIEVAVKAIKDSGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSKLATPLMDEGIFIPMS
GMVQSLNVSVAAATLLFEALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGKSYPALNDQGEIQGSLPRN
VRLRC
>Mature_245_residues
MRDTASHTLKSKDAWPMPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDAVGVLEAHAVNYSGRLPTFNS
TAQGSQKWIPLKEHSSIEVAVKAIKDSGFRLYGTHLGGNARDYRDCDFCEPTAFVLGAEKWGLSKLATPLMDEGIFIPMS
GMVQSLNVSVAAATLLFEALRQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGKSYPALNDQGEIQGSLPRN
VRLRC

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=215, Percent_Identity=46.5116279069767, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI1790623, Length=149, Percent_Identity=37.5838926174497, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 27165; Mature: 27165

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDTASHTLKSKDAWPMPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDA
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
VGVLEAHAVNYSGRLPTFNSTAQGSQKWIPLKEHSSIEVAVKAIKDSGFRLYGTHLGGNA
CCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCC
RDYRDCDFCEPTAFVLGAEKWGLSKLATPLMDEGIFIPMSGMVQSLNVSVAAATLLFEAL
CCCCCCCCCCCCCEEEECCHHCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
RQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGKSYPALNDQGEIQGSLPRN
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VRLRC
CEECC
>Mature Secondary Structure
MRDTASHTLKSKDAWPMPLLPRRFDRLKAVLDCRMADLTVLLEHVEKPHNLSAILRSCDA
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
VGVLEAHAVNYSGRLPTFNSTAQGSQKWIPLKEHSSIEVAVKAIKDSGFRLYGTHLGGNA
CCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCC
RDYRDCDFCEPTAFVLGAEKWGLSKLATPLMDEGIFIPMSGMVQSLNVSVAAATLLFEAL
CCCCCCCCCCCCCEEEECCHHCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
RQRRLAGIAPQAGEGLEPALYQQRLFEWAYPEVAAWCQQEGKSYPALNDQGEIQGSLPRN
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VRLRC
CEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]