Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 33862499

GI number: 33862499

Start: 261320

End: 262489

Strand: Reverse

Name: metB [H]

Synonym: PMT0226

Alternate gene names: 33862499

Gene position: 262489-261320 (Counterclockwise)

Preceding gene: 33862500

Following gene: 33862498

Centisome position: 10.89

GC content: 55.56

Gene sequence:

>1170_bases
GTGGCCCCAGCTCAAAACGATCAACAGCAGGAGGTTGCAGGGATCAGTACCCGCGCCATCCACCACGGCGAAAGCTTTGC
AGCAGAGACCGGTGCCGTGATGCCACCGATCTTTACCAGTTCAACCTTTGCCCACGGCAATCCTCAGGGCTTCGACTACA
CCCGTTCAGGCAATCCCAATTTCCGCATCCTCGAATCAGTGTTGGCAGCCGTAGAAAGCTGTCAATTCGCCACTGTCTTC
GCTTCAGGGGTGAGCGCCATCACAGCCATCGCCTCAACACTGCAAGCCGGAGATCTGGTGCTCTGTGAGGAAAATCTTTA
CGGCTGCACCGTGCGCTTATTCGAGCAGGTTTTTAGTCGTTTTGGTGTACAAACAGCCTGGTTGGATTTCACCAATCCAT
CAGCGCTTGATGCCATTGCAGTTCAAAAACCCGCAATGATCTGGCTGGAGAGTCCAACCAATCCCCTGCTCAAAGTGATC
GATCTAGCAGCCGTCTGTGATGCCGCTGCAGCAGAAAAAATCCCAGTTGTAGTCGACAACACGTTTGCCACAGCCCTACT
GCAACAACCACTGCAGCTGGGCGCCACCCTCTCACTCACCAGCACCACCAAATACATCAACGGCCATTCCGATGCCCTTG
GCGGTGTGGTCTGCAGCGACGACCCGCAATGGCAGCAAAAGATGGTGTTCGCACAAAAGGCCTTGGGATTACAACCCTCC
CCATTTGACGCCTGGTTGATTACGCGCGGCATCAAAACCCTGCCACTCAGGCTGGAACGCCACATCGCTAATGCTGCCGC
CATCGCTGATCAACTGGCCAGCCATCCACGCATGCACTGGGTGCGCTATCCATTGCGTTCAGACCATCCCCAGCAGGCTG
TAGCCCGCAAGCAGATGAGTGGTGGTGGTGCCATTGTCACGGTCAGTCTCGACGCTGACCAAAGCCAGACTTACGCCTTC
TGCAAGAGCCTGCGCTATTTCACCATGGCCGAAAGCCTGGGCGGTGTTGAAAGCCTCGTTTGTCATCCCGCCACCATGAC
CCATGCCTCAGTCGATCCAGATGTCAAGCGAAAACTTGGCATCGATGATGGTCTGGTGCGCTTCTCCGTGGGCTGCGAAG
ACCTCGCTGATCTCAGCAGAGATTTAGATCAAGCCCTCAAACAGCTGTAG

Upstream 100 bases:

>100_bases
AGGTTTGAATCAAAATCAGCCAAGCGTTCTTCAACAGGCCCCTGCTTTGCAGGGGTTTTTTGTTGCTGTTGCCTACCCTG
CTCAAAGCCCTCTCTAGATC

Downstream 100 bases:

>100_bases
TGAGCCCACGCGACCTACTGCAGGATCCCTGCTGGCACGCTGATGAGCTCGGCAAAGCTCTACCAGATAGTCCCCATGCC
GTTTCGGTAGCCCTACCCCG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAPAQNDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATVF
ASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMIWLESPTNPLLKVI
DLAAVCDAAAAEKIPVVVDNTFATALLQQPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
PFDAWLITRGIKTLPLRLERHIANAAAIADQLASHPRMHWVRYPLRSDHPQQAVARKQMSGGGAIVTVSLDADQSQTYAF
CKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSRDLDQALKQL

Sequences:

>Translated_389_residues
MAPAQNDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATVF
ASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMIWLESPTNPLLKVI
DLAAVCDAAAAEKIPVVVDNTFATALLQQPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
PFDAWLITRGIKTLPLRLERHIANAAAIADQLASHPRMHWVRYPLRSDHPQQAVARKQMSGGGAIVTVSLDADQSQTYAF
CKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSRDLDQALKQL
>Mature_388_residues
APAQNDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPNFRILESVLAAVESCQFATVFA
SGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSRFGVQTAWLDFTNPSALDAIAVQKPAMIWLESPTNPLLKVID
LAAVCDAAAAEKIPVVVDNTFATALLQQPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPSP
FDAWLITRGIKTLPLRLERHIANAAAIADQLASHPRMHWVRYPLRSDHPQQAVARKQMSGGGAIVTVSLDADQSQTYAFC
KSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLGIDDGLVRFSVGCEDLADLSRDLDQALKQL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=42.8571428571429, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=39.6825396825397, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=38.7267904509284, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=37.7659574468085, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789383, Length=343, Percent_Identity=25.9475218658892, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=44.4444444444444, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=43.6507936507937, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=32.6086956521739, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=39.7959183673469, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6321254, Length=393, Percent_Identity=30.7888040712468, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=28.3980582524272, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.2051282051282, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=304, Percent_Identity=26.6447368421053, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=41.9270833333333, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41886; Mature: 41755

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAQNDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPN
CCCCCCCCHHHHHCCHHHHEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FRILESVLAAVESCQFATVFASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
FGVQTAWLDFTNPSALDAIAVQKPAMIWLESPTNPLLKVIDLAAVCDAAAAEKIPVVVDN
HCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECC
TFATALLQQPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
HHHHHHHHHHHHHCCEEEECCCHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCC
PFDAWLITRGIKTLPLRLERHIANAAAIADQLASHPRMHWVRYPLRSDHPQQAVARKQMS
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCC
GGGAIVTVSLDADQSQTYAFCKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLG
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCC
IDDGLVRFSVGCEDLADLSRDLDQALKQL
CCCCEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APAQNDQQQEVAGISTRAIHHGESFAAETGAVMPPIFTSSTFAHGNPQGFDYTRSGNPN
CCCCCCCHHHHHCCHHHHEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FRILESVLAAVESCQFATVFASGVSAITAIASTLQAGDLVLCEENLYGCTVRLFEQVFSR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
FGVQTAWLDFTNPSALDAIAVQKPAMIWLESPTNPLLKVIDLAAVCDAAAAEKIPVVVDN
HCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECC
TFATALLQQPLQLGATLSLTSTTKYINGHSDALGGVVCSDDPQWQQKMVFAQKALGLQPS
HHHHHHHHHHHHHCCEEEECCCHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCC
PFDAWLITRGIKTLPLRLERHIANAAAIADQLASHPRMHWVRYPLRSDHPQQAVARKQMS
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCC
GGGAIVTVSLDADQSQTYAFCKSLRYFTMAESLGGVESLVCHPATMTHASVDPDVKRKLG
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCC
IDDGLVRFSVGCEDLADLSRDLDQALKQL
CCCCEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]