Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 33862473

GI number: 33862473

Start: 231248

End: 232258

Strand: Reverse

Name: dus1 [H]

Synonym: PMT0200

Alternate gene names: 33862473

Gene position: 232258-231248 (Counterclockwise)

Preceding gene: 33862482

Following gene: 33862470

Centisome position: 9.63

GC content: 58.26

Gene sequence:

>1011_bases
GTGCCTGAACTCAGCCTGCCCGGCAGGGGAACAACAAGGGCACTGCGCTGCAGAGTGCTGCAATCGCCCCTTGCAGGCGT
GAGTGATCAGATCTTCCGAAGCCTGGTTCGGCGCTGGGCGCCAGATGCATTGTTATTCACGGAAATGGTCAATGCCACCA
GCCTGGAGCTGGGCCACGGCCTACAGAAAATCAACGAACTCGCCAATGAAGCCGGGCCCATTGGCGTGCAATTGTTCGAT
CACCGCCCAGAAGCGATGGCCGATGCTGCACAACGAGCAGAGGCTGCTGGTGCCTTCCTGATCGACATCAACATGGGCTG
TCCTGTGCGCAAGATTGCACGCAAGGGCGGCGGCTCTGGTCTGATCCGTGACCCACAACTGGCGGCAAAAATCGTGAGCA
CTGTTGCTGCAGCGGTCAAAATCCCAGTCACCGTGAAGACAAGGCTGGGCTGGTGTGGCAGCGATGCAAAACCAATTGAA
TGGTGCCAATCACTCGAGCAGGCCGGCGCCCAGATGTTGACATTGCATGCTCGAACTCGAGAGCAAGGCTTCAAAGGCTC
AGCTGATTGGCACGCCATCGCTGCCGTCAAAAGCGCACTGCAAATCCCCGTGATTGCCAATGGCGATGTCAAGAGCGACA
TAGATGCCAAGCGCTGCCTAGCGATCACTGGAGCCGATGGCGTGATGGTGGGCAGAGGCTCGCTGGGAGCGCCATGGCTT
GTGGGCCAAATCGATGCAGCACTATCCGGCCGCCCAGTGCCTGCAACGCCTGGAGCAGCAGAGCGACTCACCATTGCTCG
TGAACAACTTGAAGCGCTGGTTCAAGCAAAGGGGGAACATGGCCTCTTGATTGCCCGTAAACACATGGGATGGACTTGTA
GCGGCTTCCCCGGCGCATCAAAACTGCGCCATGCCCTGATGCGTGCACCAACGCCAATGGATGCCATATCACTGTTAGAG
CAAGCCAGCGCAGAACTACTAACGGCATGGCCAGAAGCGACCAACGCCTAA

Upstream 100 bases:

>100_bases
TACGAAAACCAAAAATTCTTATTTCTAGAGAACGCTCCCTTGAATGAAGAGAGGCCATTCTCTCTAGGATATTGATCCGA
TCACTCTGATCATCAACATT

Downstream 100 bases:

>100_bases
TCCAGCCTCAAGTTGCGATCAAGCCGCTACCAAAGCACATCCAATCACCCAGCCAATATCACGAATCAATCGCCATTATC
AACTCAACGGCTCATCATCC

Product: nitrogen regulation protein NifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKINELANEAGPIGVQLFD
HRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDAKPIE
WCQSLEQAGAQMLTLHARTREQGFKGSADWHAIAAVKSALQIPVIANGDVKSDIDAKRCLAITGADGVMVGRGSLGAPWL
VGQIDAALSGRPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFPGASKLRHALMRAPTPMDAISLLE
QASAELLTAWPEATNA

Sequences:

>Translated_336_residues
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKINELANEAGPIGVQLFD
HRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDAKPIE
WCQSLEQAGAQMLTLHARTREQGFKGSADWHAIAAVKSALQIPVIANGDVKSDIDAKRCLAITGADGVMVGRGSLGAPWL
VGQIDAALSGRPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFPGASKLRHALMRAPTPMDAISLLE
QASAELLTAWPEATNA
>Mature_335_residues
PELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHGLQKINELANEAGPIGVQLFDH
RPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSGLIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDAKPIEW
CQSLEQAGAQMLTLHARTREQGFKGSADWHAIAAVKSALQIPVIANGDVKSDIDAKRCLAITGADGVMVGRGSLGAPWLV
GQIDAALSGRPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFPGASKLRHALMRAPTPMDAISLLEQ
ASAELLTAWPEATNA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=265, Percent_Identity=33.9622641509434, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI239788483, Length=242, Percent_Identity=31.404958677686, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=34.2342342342342, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=31.3559322033898, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI8923374, Length=144, Percent_Identity=34.0277777777778, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789660, Length=297, Percent_Identity=37.037037037037, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788462, Length=264, Percent_Identity=35.6060606060606, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=29.4670846394984, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=254, Percent_Identity=30.3149606299213, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=30.7377049180328, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=31.4814814814815, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=32.1888412017167, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.646017699115, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=27.5080906148867, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=34.2222222222222, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=34.2222222222222, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=33.4745762711864, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=35.0649350649351, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=155, Percent_Identity=36.7741935483871, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35494; Mature: 35363

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHG
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQKINELANEAGPIGVQLFDHRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSG
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCC
LIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDAKPIEWCQSLEQAGAQMLTLHARTR
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHEEEEHHHHH
EQGFKGSADWHAIAAVKSALQIPVIANGDVKSDIDAKRCLAITGADGVMVGRGSLGAPWL
HCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCH
VGQIDAALSGRPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFPGAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHH
KLRHALMRAPTPMDAISLLEQASAELLTAWPEATNA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PELSLPGRGTTRALRCRVLQSPLAGVSDQIFRSLVRRWAPDALLFTEMVNATSLELGHG
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQKINELANEAGPIGVQLFDHRPEAMADAAQRAEAAGAFLIDINMGCPVRKIARKGGGSG
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCC
LIRDPQLAAKIVSTVAAAVKIPVTVKTRLGWCGSDAKPIEWCQSLEQAGAQMLTLHARTR
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHEEEEHHHHH
EQGFKGSADWHAIAAVKSALQIPVIANGDVKSDIDAKRCLAITGADGVMVGRGSLGAPWL
HCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCH
VGQIDAALSGRPVPATPGAAERLTIAREQLEALVQAKGEHGLLIARKHMGWTCSGFPGAS
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHH
KLRHALMRAPTPMDAISLLEQASAELLTAWPEATNA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]