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The map label for this gene is sqdB [C]

Identifier: 33862323

GI number: 33862323

Start: 50930

End: 52126

Strand: Reverse

Name: sqdB [C]

Synonym: PMT0050

Alternate gene names: 33862323

Gene position: 52126-50930 (Counterclockwise)

Preceding gene: 33862324

Following gene: 33862322

Centisome position: 2.16

GC content: 54.05

Gene sequence:

>1197_bases
TTGAAAGTTTTCGTTCTCGGCGGCGACGGCTTCTGCGGATGGCCTTGTTCAGTGAACCTTGCTGATAGGGGTCACGACGT
ATTGATCGTCGACAATCTCAGCCGCCGCAAGATCGATATCGATCTTGAAGTCGAGTCACTGACACCAATCACCAGCATTG
GCGACCGCCTTAAAGCCTGGCGAGAAATCGGAGGCAAGCCAATTCGTTTTGAGCACCTAGACATCGCCCATCAGTACGAC
CGATTGGTGACAATGCTCAAAACTGAACGACCCGATGCGGTGGTTCACTTTGCCGAGCAGCGTGCAGCGCCCTACTCGAT
GAAGAGCAGCTCCACCAAGCGCTATACCGTCGATAACAACGTCAACGGCACTCACAATCTGCTCGCCGCCATCGTTGAAA
GCGGCCTAGACATCCACATCGTGCACCTGGGAACGATGGGGGTGTACGGCTACGGCTCGCACCGCGGAGCCACCATTCCT
GAGGGATACCTCAAAGTGGAAGTGCCCCAGCCTGATGGCAGCCGTTTCGAGGAAGAAATTCTTCACCCAGCCAGCCCTGG
AAGCGTCTATCACATGACCAAGACGCTCGATCAACTGCTTTTCCTCTACTACAACAAGAATGACCAAATCAGGATCACCG
ACCTTCACCAAGGCATCGTCTGGGGTACCAACACTGAAGCAACCGCTAGGGATCCACGACTAACCAACCGCTTTGATTAC
GACGGTGACTACGGCACAGTTCTCAACCGCTTCCTGATGCAGGCAGCAATCGGTTATCCGCTCACTGTCCATGGAACAGG
AGGTCAAACCCGTGCCTTCATCCACATCTGCGATTCTGTGAAGTGCGTACAACTGGCTCTTGAAAACCCACCGGCTAAAG
GCGAACGAGTCAAGATTTTCAACCAAATGACCGAGAGCCACCAAGTAGGTGAACTTGCCAAGAAGGTAGCTGCCCTAACC
GGCGCCGAACTAAACCATCTTCCAAACCCACGCAACGAAGCAGTGGAGAATGATCTGATTGTGGATAACCGCTGCTTCAT
CGAACTTGGACTCAAACCAACCACCCTCGACGATGGCCTACTTGCTGAAGTAGTCGATGTGGCAAAACGCTGGGCAGACC
GCTGCGACCGCAGTCGTATCCCATGTGTATCGGCCTGGACTTCGACCCAAGCGGAGGCCATCAAGAACCCCTCCTAA

Upstream 100 bases:

>100_bases
TTGTGACATCCCTAATCATCGTCAGGATCGCCATCAGCTGAAAAACCTTGTAGTAGCTTGCCCTGACTGAGCGAATTGCT
CGTTCCTGCAGGAGTTTGAG

Downstream 100 bases:

>100_bases
ACAGCCTGCCCACACCACCCGCTGAACCAGTGAAGATCGCCTTTTTCACAGAGACCTTCCTCCCAAAAGTGGATGGAATC
GTGACAAGACTCACCAAAAC

Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; UDP Sulfoquinovose Synthase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVFVLGGDGFCGWPCSVNLADRGHDVLIVDNLSRRKIDIDLEVESLTPITSIGDRLKAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKNPS

Sequences:

>Translated_398_residues
MKVFVLGGDGFCGWPCSVNLADRGHDVLIVDNLSRRKIDIDLEVESLTPITSIGDRLKAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKNPS
>Mature_398_residues
MKVFVLGGDGFCGWPCSVNLADRGHDVLIVDNLSRRKIDIDLEVESLTPITSIGDRLKAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKNPS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44268; Mature: 44268

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFVLGGDGFCGWPCSVNLADRGHDVLIVDNLSRRKIDIDLEVESLTPITSIGDRLKAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
REIGGKPIRFEHLDIAHQYDRLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNN
HHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEHHH
NQMTESHQVGELAKKVAALTGAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCCHH
LAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKNPS
HHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MKVFVLGGDGFCGWPCSVNLADRGHDVLIVDNLSRRKIDIDLEVESLTPITSIGDRLKAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
REIGGKPIRFEHLDIAHQYDRLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNN
HHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEHHH
NQMTESHQVGELAKKVAALTGAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCCHH
LAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKNPS
HHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA