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The map label for this gene is pcnB [H]

Identifier: 326423862

GI number: 326423862

Start: 1612406

End: 1613767

Strand: Reverse

Name: pcnB [H]

Synonym: NA

Alternate gene names: 326423862

Gene position: 1613767-1612406 (Counterclockwise)

Preceding gene: 326423863

Following gene: 27365006

Centisome position: 49.17

GC content: 48.75

Gene sequence:

>1362_bases
ATGAATAAAAACGAATATACAACATGCGATTCCACCACGTGTCCGGAACTCGCTTTAAATGTCATTACTCGTCAAGAGCA
CAATATTTCGCGCAAGCAGATTAGCGACCATGCGCTAAAAGTACTGTACCGACTTCATGGTGCCGGCTTCGACGCTTATC
TTGTTGGCGGTGGCGTACGTGATTTACTGCTGGGACAAAGCCCAAAAGATTTTGATATTGCAACCAACGCGACACCTGAG
CAGCTAAAGCAGTTATTCCGTAACTGCCGCCTGATTGGTCGCCGCTTTCGACTAGCGCATATCATGTTTGGTCGCGAAAT
CATTGAAGTGGCCACGTTCCGTGGACACCACCAAGATGCCAATTCGCAGATCTCCCAGCAGTCAAAAGAAGGCATGCTGT
TGCGTGATAACGTGTATGGCACCATTGATGAAGATGCTGAACGACGTGACTTCACCATCAATGCGATGTACTACAACATT
GCAGATTACTCGATCCATGATTATGCAGGTGGTGTTGAAGACCTTGAAGATCGCTTAGTACGCTTAATTGGCGATCCTGA
GACTCGCTATCGTGAAGATCCTGTACGTATGCTTCGCGCGATTCGATTTGCCGCCAAGCTCGACTTCGATATCGAAGAAG
ACACGGCCGCACCGATTGAAGAGATGGCGTACTTGTTAAGAGACATCCCTGCCGCGCGTTTATACGAAGAATCGCTTAAG
CTACTGCAATCGGGCCATGGCTTGGAAACGTATCACCTCATGCGTGAATACAATCTATTCCAACAAATGTTCCCAGCGAT
CTCAGCTTATTTCACCGAGGATTACTCTTCCAAAGCTGAGCAGATGTTGGACTTAGTCTTAGATTCCACTGACATTCGTG
TCGAAGAAGGCAAGCGCATTAATCCCGCTTACATGTTTGCGGCTATGCTGTGGTATCCGCTGCTTGAGCTGGCAGACAAA
TTGATGGATAGCCACAATTTGTGTCACTACGACGCCGTCATGGAAGCGAGCAACCTGATCCTTGATGAACAGGTGCGCTC
GATAGCTATTCCGCGCCGCCACACCGCGACCATCCGTGAAATTTGGCAACTGCAACTGCGTCTACCTCGTCGCAACGGTA
AACGTGCTTTCCGCCTAATGGAACTCAACAAATTCCGTGCTGGTTTTGACTTCCTAGAAATGCGAGGAGAGATCGAAGGC
GGTGAAACGGCAACATTGGCCAAGTGGTGGGGCACGTTCCAAAATGCAGGTCGTGAAATGCGCCTAGCGATGGTAAACGA
TCTCGAAGGCCCTGCTGGCAGTTCAAAATCCGGCCGTAAGCGTCGTTCTTTTAATAAGAAAAAGAGCAAAGCCAAATCAT
GA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: Poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:
NA

Sequences:

>Translated_453_residues
MNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAYLVGGGVRDLLLGQSPKDFDIATNATPE
QLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNI
ADYSIHDYAGGVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAYLLRDIPAARLYEESLK
LLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRINPAYMFAAMLWYPLLELADK
LMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEG
GETATLAKWWGTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
>Mature_453_residues
MNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAYLVGGGVRDLLLGQSPKDFDIATNATPE
QLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNI
ADYSIHDYAGGVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAYLLRDIPAARLYEESLK
LLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRINPAYMFAAMLWYPLLELADK
LMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEG
GETATLAKWWGTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=220, Percent_Identity=32.7272727272727, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI87081691, Length=414, Percent_Identity=59.1787439613527, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1789436, Length=238, Percent_Identity=28.9915966386555, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=188, Percent_Identity=29.2553191489362, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=292, Percent_Identity=28.7671232876712, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357337, Length=292, Percent_Identity=28.7671232876712, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52258; Mature: 52258

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAYLVGGGVR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
DLLLGQSPKDFDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAGGVEDLEDRLV
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCCCHHHHHHHHH
RLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAYLLRDIPAARLYEESLK
HHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
NPAYMFAAMLWYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIRE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEGGETATLAKWWGTFQNAGREM
HHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCE
RLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
EHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAYLVGGGVR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
DLLLGQSPKDFDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAGGVEDLEDRLV
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCCCHHHHHHHHH
RLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAYLLRDIPAARLYEESLK
HHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
NPAYMFAAMLWYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIRE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEGGETATLAKWWGTFQNAGREM
HHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCE
RLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
EHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]