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The map label for this gene is mrcA [H]

Identifier: 326423829

GI number: 326423829

Start: 1359786

End: 1362299

Strand: Reverse

Name: mrcA [H]

Synonym: NA

Alternate gene names: 326423829

Gene position: 1362299-1359786 (Counterclockwise)

Preceding gene: 27364785

Following gene: 326423828

Centisome position: 41.51

GC content: 48.29

Gene sequence:

>2514_bases
GTGAAGTTCATAAAGCGTTTACTTATATTTGCATTAATTTGCATAATTCTTGGAGTCACAACAATTTTTGGCTTTTATCA
GTACGTTAAGCCAGAACTGCCAGATGTGGCGACACTCAAAAATGTTGAGTTACAAACTCCGATGCAGGTGTTTAGCCAAG
ATGGGAAATTAATTGCGCAATTTGGTGAAAAGCGTCGCATTCCAGTGACCTACGACCAAATTCCTCAATCGCTGATCCAC
GCGCTGATCGCGACTGAAGATAGCCGTTTCTATGATCATTACGGTATCGACCCGATTGGTATTACCCGAGCGGCGTTAGT
TGTCGCGATGTCAGGTTCCGCTAAACAGGGCGCAAGTACCATTACTCAGCAATTGGCACGTAACTTCTTCCTCTCGAATG
AAAAGAAGTTAATGCGTAAAGTGAAAGAAATTTTTATCGCCATCCATATTGAGCAACTGCTGAGCAAGCAGGAGATCATG
GAGCTTTACGTTAATAAAATTTTCCTTGGTTATCGCTCATACGGTTTTGGGGCGGCAGCTCGAGTCTATTTTGGCAAAGA
TTTGCAAGATCTCTCCTTAAGCGAGATTGCGACTTTGGCGGGTATGCCAAAAGCACCATCGACACTGAACCCTATCTATT
CGCTAGAACGCGCAACCAATCGTCGTAACGTCGTCCTAATGCGCATGTTGGATGAAAAATACATCACCCAAGAAGAGTAT
GACGCAGCACGAGCGGAAACATTGATTTCTCAATTCCACGGCGCGGAGATCGAGGTCAGTGCTCCTTATGTGGCTGAGCT
CGCGCGTGCATGGATGGTACAGGAGTACGGCGAAGAGAAAGCGTATACGTCCGGTATGAATGTCTACATGACCATCGACT
CTAAGCTCCAAGCCGCAGCCAACAAAGCCGCGATTGACAACTTATTAGCCTACGATGAGCGTCACGGCTACCGTGGTGAA
GAAAGTGTGCTGTGGAAAGCAGGCGAAGCCGCTTGGGATGAAGCGAAGATGGACGATCATCTGAGCAACCAACCGACTTA
CGGCGAATTGATTCCAGCGATTGTGACCAAGGTTGAAAGCAAAAACGCAACCATTTGGGTTAAACGTGAACAAAGCCAAA
CCATCGCATGGTCAGGCATGAACTGGGCGCGCAAGTTCATCACTGATGAACGTCAAGGCAGTGCACCAAACTCAGCCAAA
GACATCTTAGCGCCGGGCGAGCAAATTTGGGTTCGCAAAGTAGTGACTACCGATGACAAAGGCGAAAGCCAGGAAAGCTG
GCACTTAAGTCAAGTGCCTAACGCCAATACCGCCTTTATCGCCATGAACCCTGATAATGGCGCGGTACTTTCCCTCGTGG
GTGGCTTTAACTTTGTTCACAACAAGTTTAACCGTGCCACCATGTCGGTTCGCCAAGTAGGTTCGAGTATCAAACCCTTT
ATTTACTCGGCGGCGATTGATAACGGCATGACTCTGGCCACGCTGATCAACGATGCACCAATCAACAAATGGGATGAGAG
CCAAGGTACAGCATGGCGTCCTAAGAACTCTCCTCCGACTTACATTGGCCCAACCCGTTTACGTATTGGCTTAGCAACGT
CGAAAAACGTCATGGCGGTTCGTACTCTGCGTGAAGTCGGGCTGGATGAAACGCGTGAATACCTCACTCGCTTTGGTTTT
GAGCTCGACCAACTGCCACGTTCAGAAACCATTGCCTTGGGTGCAGGTAGCTTAACCCCAATGAAGATGGCACAGGGATT
CTCGGTTTTTGCCAATGGGGGCTACTATGTAGAGCCTTATTACATCAGCCATATTGAAGATCCTTATGGCAATGTCGTGT
TTGAGTCACAGCCACAAACCATCTGTCAGCAAGATTGCCCAGTGGCCACGCAAACCATTGTAGAGAATGAGAATGGTGAA
AGCACCGAGAGCACTCAGCCATTTGCCAAGCAAGTGATCAGCGAACAGAATGCTTTTATCATGCGCGAAATGATGTACAG
CAACATCTGGGGGGGTGGTAACTGGCGTGAAGGTACAGGTTGGAATGGCACAGGCTGGCGTGCACAAACGTTGGGTCGAC
GCGATATCGGCGGTAAAACCGGTACAACCAACGATTCCAAAGACGCTTGGTATAACGGTTATGGCCCGGGTGTGGTCGCG
ATTTCTTGGGTAGGTTTTGATGACCATAGTCGCACGCTCGGAAAAACCACCACTAACGCCAACTTAGATGGTAAACAAAC
CTCTGGGGCCGAATCTGGAGCGAAAACTGCATTGCCAGCGTGGATTGATTTTATGAAACTGGCTTTAGAAGGCGTGCCTG
AACATGAAAAAGTGTTACCCGCGAATATAGTGCGTGTGCGCATCGATCGTGAAACCGGGCTATTAACCAACAAGACCGAT
GACAGCGCTATGTTTGAGTACTTCATTAAAGGGACAGAGCCGACTGAGTATGTTTCTAGCGTCGAGGAAGAAAGCATCTA
TTCCTCTTCCAGTGATGAAGAAGCGCTGTTCTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: Multimodular transpeptidase-transglycosylase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 837; Mature: 837

Protein sequence:
NA

Sequences:

>Translated_837_residues
MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQFGEKRRIPVTYDQIPQSLIH
ALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGASTITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIM
ELYVNKIFLGYRSYGFGAAARVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY
DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAANKAAIDNLLAYDERHGYRGE
ESVLWKAGEAAWDEAKMDDHLSNQPTYGELIPAIVTKVESKNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAK
DILAPGEQIWVRKVVTTDDKGESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF
IYSAAIDNGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAVRTLREVGLDETREYLTRFGF
ELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPYYISHIEDPYGNVVFESQPQTICQQDCPVATQTIVENENGE
STESTQPFAKQVISEQNAFIMREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA
ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLPANIVRVRIDRETGLLTNKTD
DSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF
>Mature_837_residues
MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQFGEKRRIPVTYDQIPQSLIH
ALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGASTITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIM
ELYVNKIFLGYRSYGFGAAARVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY
DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAANKAAIDNLLAYDERHGYRGE
ESVLWKAGEAAWDEAKMDDHLSNQPTYGELIPAIVTKVESKNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAK
DILAPGEQIWVRKVVTTDDKGESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF
IYSAAIDNGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAVRTLREVGLDETREYLTRFGF
ELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPYYISHIEDPYGNVVFESQPQTICQQDCPVATQTIVENENGE
STESTQPFAKQVISEQNAFIMREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA
ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLPANIVRVRIDRETGLLTNKTD
DSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=849, Percent_Identity=52.2968197879859, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI1786343, Length=296, Percent_Identity=35.1351351351351, Blast_Score=152, Evalue=1e-37,
Organism=Escherichia coli, GI1788867, Length=202, Percent_Identity=37.1287128712871, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=32.3863636363636, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 93282; Mature: 93282

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCCCHHHHH
FGEKRRIPVTYDQIPQSLIHALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGAST
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHEEECCCCCCHHHH
ITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIMELYVNKIFLGYRSYGFGAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
RVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHCCHHHH
DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAA
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECHHHHHHH
NKAAIDNLLAYDERHGYRGEESVLWKAGEAAWDEAKMDDHLSNQPTYGELIPAIVTKVES
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
KNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAKDILAPGEQIWVRKVVTTDDK
CCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHEEEEEEECCCC
GESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF
CCCCCCEEHHCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
IYSAAIDNGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAV
EEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHH
RTLREVGLDETREYLTRFGFELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPY
HHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE
YISHIEDPYGNVVFESQPQTICQQDCPVATQTIVENENGESTESTQPFAKQVISEQNAFI
EEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHHCCCHHHH
MREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLP
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
ANIVRVRIDRETGLLTNKTDDSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF
CEEEEEEEECCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCCCHHHHH
FGEKRRIPVTYDQIPQSLIHALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGAST
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHEEECCCCCCHHHH
ITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIMELYVNKIFLGYRSYGFGAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
RVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHCCHHHH
DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAA
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECHHHHHHH
NKAAIDNLLAYDERHGYRGEESVLWKAGEAAWDEAKMDDHLSNQPTYGELIPAIVTKVES
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
KNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAKDILAPGEQIWVRKVVTTDDK
CCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHEEEEEEECCCC
GESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF
CCCCCCEEHHCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
IYSAAIDNGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAV
EEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHH
RTLREVGLDETREYLTRFGFELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPY
HHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE
YISHIEDPYGNVVFESQPQTICQQDCPVATQTIVENENGESTESTQPFAKQVISEQNAFI
EEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHHCCCHHHH
MREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLP
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
ANIVRVRIDRETGLLTNKTDDSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF
CEEEEEEEECCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]