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The map label for this gene is dusB [H]

Identifier: 326423811

GI number: 326423811

Start: 1210886

End: 1211854

Strand: Reverse

Name: dusB [H]

Synonym: NA

Alternate gene names: 326423811

Gene position: 1211854-1210886 (Counterclockwise)

Preceding gene: 27364635

Following gene: 27364633

Centisome position: 36.93

GC content: 48.09

Gene sequence:

>969_bases
TTGAAGATCGGAAATTATCAACTTAAGAACAAACTAATCGTAGCGCCGATGGCCGGAGTAACGGATAGACCGTTTCGAGA
GTTGTGCCTTCGTTATGGTGCGGGAATGGCCGTCAGTGAAATGATGTCCTCTAATCCGCAGCTATGGAAAACGTCGAAGT
CGAAGCAACGCATGGTGCATGAAGGCGAATCGGGCATTCGCTCTGTACAAATCGCGGGCAGCGATCCACAGCTGATGGCA
GATGCTGCGCAATTCAGTGTTGAAAATGGTGCGCAAATCATCGATATCAACATGGGTTGCCCAGCAAAAAAAGTGAATAA
AAAGCTGGCGGGTTCCGCGCTGCTCAAATACCCGGACATTATCGAACAGATTTTGAAAGCGGTGGTTGACGCAGTGGACG
TTCCTGTAACGTTGAAAACCCGCACTGGCTGGGATACAGAAAACAAAAACTGTATTCACATCGCTAAATTAGCCGAAGAC
TGCGGCATACAAGCTCTTGCTCTGCATGGCAGAACCAAAGCCTGTATGTACAAAGGTGAGGCCGAATACGACAGCATTAA
AGCCGTTAAACAGGCAGTTTCCATTCCGGTTATCGCAAACGGTGATATCGATAGCCCGGAAAAAGCCAAGTATGTACTGG
AGTACACCGGCGCAGACGCTTTAATGATTGGACGCCCTGCCCAGGGTCGTCCTTGGATTTTCCAGGAAATCCATCACTAT
TTGGAAAACGGCACCACAATGCCTGAACTTCCGATTGAGGAAGTAAAAAGCATTATGCTCGGTCATGTTCAGGCTCTCCA
TGAGTTTTATGGTGAGTATTTAGGCCCTCGCATCGCGCGTAAGCACGTGGGTTGGTATCTAAAAGAACATGAGCAAGCGA
GTGAGTTTCGCCGTACCTTCAACGCTATCGACGCAGCTATGCTGCAACTTGAAGCGCTCGAAGGTTATTTTGATAACGTT
GCATCATAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: tRNA dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:
NA

Sequences:

>Translated_322_residues
MKIGNYQLKNKLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPQLWKTSKSKQRMVHEGESGIRSVQIAGSDPQLMA
DAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLKYPDIIEQILKAVVDAVDVPVTLKTRTGWDTENKNCIHIAKLAED
CGIQALALHGRTKACMYKGEAEYDSIKAVKQAVSIPVIANGDIDSPEKAKYVLEYTGADALMIGRPAQGRPWIFQEIHHY
LENGTTMPELPIEEVKSIMLGHVQALHEFYGEYLGPRIARKHVGWYLKEHEQASEFRRTFNAIDAAMLQLEALEGYFDNV
AS
>Mature_322_residues
MKIGNYQLKNKLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPQLWKTSKSKQRMVHEGESGIRSVQIAGSDPQLMA
DAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLKYPDIIEQILKAVVDAVDVPVTLKTRTGWDTENKNCIHIAKLAED
CGIQALALHGRTKACMYKGEAEYDSIKAVKQAVSIPVIANGDIDSPEKAKYVLEYTGADALMIGRPAQGRPWIFQEIHHY
LENGTTMPELPIEEVKSIMLGHVQALHEFYGEYLGPRIARKHVGWYLKEHEQASEFRRTFNAIDAAMLQLEALEGYFDNV
AS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=34.6153846153846, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=34.7457627118644, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=32.15859030837, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=32.5396825396825, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=29.0748898678414, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=70.0934579439252, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1788462, Length=298, Percent_Identity=32.2147651006711, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI145693211, Length=296, Percent_Identity=25.3378378378378, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=33.0612244897959, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=301, Percent_Identity=28.2392026578073, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=267, Percent_Identity=32.2097378277154, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=247, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=318, Percent_Identity=25.7861635220126, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=26.7543859649123, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=32.1138211382114, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921524, Length=241, Percent_Identity=28.6307053941909, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35680; Mature: 35680

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGNYQLKNKLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPQLWKTSKSKQRMVH
CCCCCEECCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHH
EGESGIRSVQIAGSDPQLMADAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLKYPDI
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
IEQILKAVVDAVDVPVTLKTRTGWDTENKNCIHIAKLAEDCGIQALALHGRTKACMYKGE
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEHHHHHHHHCCCEEEEECCCCHHEEECCC
AEYDSIKAVKQAVSIPVIANGDIDSPEKAKYVLEYTGADALMIGRPAQGRPWIFQEIHHY
CCHHHHHHHHHHHCCCEEECCCCCCHHHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LENGTTMPELPIEEVKSIMLGHVQALHEFYGEYLGPRIARKHVGWYLKEHEQASEFRRTF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NAIDAAMLQLEALEGYFDNVAS
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGNYQLKNKLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSSNPQLWKTSKSKQRMVH
CCCCCEECCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHH
EGESGIRSVQIAGSDPQLMADAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLKYPDI
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
IEQILKAVVDAVDVPVTLKTRTGWDTENKNCIHIAKLAEDCGIQALALHGRTKACMYKGE
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEHHHHHHHHCCCEEEEECCCCHHEEECCC
AEYDSIKAVKQAVSIPVIANGDIDSPEKAKYVLEYTGADALMIGRPAQGRPWIFQEIHHY
CCHHHHHHHHHHHCCCEEECCCCCCHHHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LENGTTMPELPIEEVKSIMLGHVQALHEFYGEYLGPRIARKHVGWYLKEHEQASEFRRTF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NAIDAAMLQLEALEGYFDNVAS
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]