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The map label for this gene is pycA [H]

Identifier: 32267348

GI number: 32267348

Start: 1770994

End: 1772418

Strand: Direct

Name: pycA [H]

Synonym: HH1849

Alternate gene names: 32267348

Gene position: 1770994-1772418 (Clockwise)

Preceding gene: 32267347

Following gene: 32267349

Centisome position: 98.44

GC content: 39.02

Gene sequence:

>1425_bases
ATGTTTCAGAAGATTCTCATTGCTAATCGTGGCGAAATTGCAGTGCGTATTATCCGCGCTTGTAGGGATTTACATATTAA
AAGTATAGCCATATATGCAAGAGCTGATAGAGATTGTTTGCACGTAAAAATGGCTGATGAATCTTATGAAATCAGTGATG
ACCCATTGCAAGGCTATCTTGATGCGGATTTGATTATTGAGGTAGCATTACGCGCTGAAGCTGATGCAATTCACCCCGGC
TATGGTTTTTTAAGTGAGAATGCCACATTTGCTAAAAAAGTGCAAGAAGCGGGAATTACTTGGATAGGACCTGATGCTCT
AACAATTGCAAAAATGGGCGATAAAAATGCAGCAAGAGCTATTATGGAAAAAAATGGAATCCCCATTGTGCCAGGCACTC
AACCACTTAATAAGCACTCTATAAGTGATATTGCTAAATATGCGCAAAAAATTGGCTATCCTGTGATACTTAAAGCAAGT
AGTGGTGGAGGTGGGCGTGGCATACGCGTAGTAGAAAAAGAAGAGGATTTAATAGAATCTTTTGAGGCGTGTAAACGCGA
AGCTATGGCATTTTTCAAAAATGATGATGTTTTTATGGAAAAATATATCGTTAATCCTAGACATATAGAGTTTCAAATCT
TAGCGGATAATTATGGTAATGTGATTCATCTGCTTGAGCGTGATTGTTCCATACAAAGACGACATCAAAAGCTTGTTGAA
ATCGCTCCTAGTCCCCTTATTAGCGATGATTTACGCCGAAGAATGGGAGCTGCTGCCGTGGCTGCCGCTAAAGCTGCACA
ATACACAAATGCAGGCACGGTTGAATTTTTGCTTGATGAGAACAATACTTTTTATTTTATGGAAATGAATACACGCATAC
AGGTTGAGCACGGCGTTACAGAGGAAATTACTGGTTATGACCTTATAGGGAGACAAATCCGCATTGCGCAAGGAGAAATT
TTAGACCTTACACAACACGATATACGCGCACAAGGCTTTGCTATTGAAGTGAGAATTAATGCTGAAGATGTGGCTAATGA
TTTTGTGCCAAATCCGGGCAAAATTACCACTTATTACCCTGCTTTAGGACCTTTTGTGCGAGTAGATAGTTGTATTTATA
AAGATTATGTGATTCCACCCTTTTATGATTCTATGGTAGCAAAGCTCATTGTGAAAGCTTCAAGCTATAATCTTGCGGTA
AATAAACTCTCGCGAGCTTTGAATGAATTTACCATTAAAGGCGTGAAAACAACAATTCCTTTTTTGATAAACATTTGCAA
TGATAAAGACTTTAGGCGAGGATATTTTGATACTTCTTATATTGAAAGTAAAATTAAAGAGCTTATGCCCCCCCCACAAT
CTAAGCCTGAAGATGATATGGTAAGCGTCATTGTCGCGGCCTTAAGCGCACGCTATGGAGCATAA

Upstream 100 bases:

>100_bases
AATACAAAGACAAAACTGCTGCACAAGAGATTTTAGATAAATCAATCAAAGCCTACAAAGGCTAAAAATACTCTTAAAAT
GATGTTTAAAGGAGGTTATG

Downstream 100 bases:

>100_bases
AACAATGGAGTAGCCTATGAAAAATATCTATCAAAGCACAATATTTTTGGATAAAAGAGCGTGTGAAAAATATCATCTTA
CACCTGAAATTCTTATGGAA

Product: acetyl-CoA carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MFQKILIANRGEIAVRIIRACRDLHIKSIAIYARADRDCLHVKMADESYEISDDPLQGYLDADLIIEVALRAEADAIHPG
YGFLSENATFAKKVQEAGITWIGPDALTIAKMGDKNAARAIMEKNGIPIVPGTQPLNKHSISDIAKYAQKIGYPVILKAS
SGGGGRGIRVVEKEEDLIESFEACKREAMAFFKNDDVFMEKYIVNPRHIEFQILADNYGNVIHLLERDCSIQRRHQKLVE
IAPSPLISDDLRRRMGAAAVAAAKAAQYTNAGTVEFLLDENNTFYFMEMNTRIQVEHGVTEEITGYDLIGRQIRIAQGEI
LDLTQHDIRAQGFAIEVRINAEDVANDFVPNPGKITTYYPALGPFVRVDSCIYKDYVIPPFYDSMVAKLIVKASSYNLAV
NKLSRALNEFTIKGVKTTIPFLINICNDKDFRRGYFDTSYIESKIKELMPPPQSKPEDDMVSVIVAALSARYGA

Sequences:

>Translated_474_residues
MFQKILIANRGEIAVRIIRACRDLHIKSIAIYARADRDCLHVKMADESYEISDDPLQGYLDADLIIEVALRAEADAIHPG
YGFLSENATFAKKVQEAGITWIGPDALTIAKMGDKNAARAIMEKNGIPIVPGTQPLNKHSISDIAKYAQKIGYPVILKAS
SGGGGRGIRVVEKEEDLIESFEACKREAMAFFKNDDVFMEKYIVNPRHIEFQILADNYGNVIHLLERDCSIQRRHQKLVE
IAPSPLISDDLRRRMGAAAVAAAKAAQYTNAGTVEFLLDENNTFYFMEMNTRIQVEHGVTEEITGYDLIGRQIRIAQGEI
LDLTQHDIRAQGFAIEVRINAEDVANDFVPNPGKITTYYPALGPFVRVDSCIYKDYVIPPFYDSMVAKLIVKASSYNLAV
NKLSRALNEFTIKGVKTTIPFLINICNDKDFRRGYFDTSYIESKIKELMPPPQSKPEDDMVSVIVAALSARYGA
>Mature_474_residues
MFQKILIANRGEIAVRIIRACRDLHIKSIAIYARADRDCLHVKMADESYEISDDPLQGYLDADLIIEVALRAEADAIHPG
YGFLSENATFAKKVQEAGITWIGPDALTIAKMGDKNAARAIMEKNGIPIVPGTQPLNKHSISDIAKYAQKIGYPVILKAS
SGGGGRGIRVVEKEEDLIESFEACKREAMAFFKNDDVFMEKYIVNPRHIEFQILADNYGNVIHLLERDCSIQRRHQKLVE
IAPSPLISDDLRRRMGAAAVAAAKAAQYTNAGTVEFLLDENNTFYFMEMNTRIQVEHGVTEEITGYDLIGRQIRIAQGEI
LDLTQHDIRAQGFAIEVRINAEDVANDFVPNPGKITTYYPALGPFVRVDSCIYKDYVIPPFYDSMVAKLIVKASSYNLAV
NKLSRALNEFTIKGVKTTIPFLINICNDKDFRRGYFDTSYIESKIKELMPPPQSKPEDDMVSVIVAALSARYGA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=448, Percent_Identity=44.8660714285714, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI106049528, Length=447, Percent_Identity=47.427293064877, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI106049295, Length=447, Percent_Identity=47.427293064877, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI106049292, Length=447, Percent_Identity=47.427293064877, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI189095269, Length=443, Percent_Identity=44.2437923250564, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI65506442, Length=443, Percent_Identity=44.2437923250564, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI295821183, Length=443, Percent_Identity=44.2437923250564, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI38679971, Length=534, Percent_Identity=29.0262172284644, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI38679974, Length=534, Percent_Identity=29.0262172284644, Blast_Score=222, Evalue=8e-58,
Organism=Homo sapiens, GI38679960, Length=534, Percent_Identity=29.0262172284644, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI38679977, Length=534, Percent_Identity=29.0262172284644, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI38679967, Length=534, Percent_Identity=29.0262172284644, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI134142062, Length=531, Percent_Identity=29.0018832391714, Blast_Score=218, Evalue=7e-57,
Organism=Escherichia coli, GI1789654, Length=446, Percent_Identity=51.1210762331839, Blast_Score=418, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17562816, Length=447, Percent_Identity=47.2035794183445, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71987519, Length=471, Percent_Identity=43.312101910828, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17567343, Length=477, Percent_Identity=42.3480083857442, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71997168, Length=443, Percent_Identity=30.6997742663657, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI71997163, Length=443, Percent_Identity=30.4740406320542, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI133931226, Length=522, Percent_Identity=28.9272030651341, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6319695, Length=447, Percent_Identity=46.7561521252796, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321376, Length=447, Percent_Identity=47.2035794183445, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319685, Length=446, Percent_Identity=40.8071748878924, Blast_Score=334, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324343, Length=538, Percent_Identity=30.1115241635688, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6323863, Length=474, Percent_Identity=31.4345991561181, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24652212, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652210, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652214, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19921944, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652216, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281363050, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652224, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652222, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652220, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652218, Length=447, Percent_Identity=48.993288590604, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651757, Length=451, Percent_Identity=43.6807095343681, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651759, Length=415, Percent_Identity=43.3734939759036, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI161076409, Length=523, Percent_Identity=28.6806883365201, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24586458, Length=523, Percent_Identity=28.6806883365201, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI161076407, Length=523, Percent_Identity=28.6806883365201, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24586460, Length=523, Percent_Identity=28.6806883365201, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52951; Mature: 52951

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKILIANRGEIAVRIIRACRDLHIKSIAIYARADRDCLHVKMADESYEISDDPLQGYL
CCCEEEEECCCHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
DADLIIEVALRAEADAIHPGYGFLSENATFAKKVQEAGITWIGPDALTIAKMGDKNAARA
CHHHHEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEECCCCHHHHH
IMEKNGIPIVPGTQPLNKHSISDIAKYAQKIGYPVILKASSGGGGRGIRVVEKEEDLIES
HHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHH
FEACKREAMAFFKNDDVFMEKYIVNPRHIEFQILADNYGNVIHLLERDCSIQRRHQKLVE
HHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEEEEECCCCCEEEHHHHCCHHHHHHHHHHH
IAPSPLISDDLRRRMGAAAVAAAKAAQYTNAGTVEFLLDENNTFYFMEMNTRIQVEHGVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCEEEEECCCC
EEITGYDLIGRQIRIAQGEILDLTQHDIRAQGFAIEVRINAEDVANDFVPNPGKITTYYP
CCCCCHHHCCCEEEECCCCEEECHHHHHCCCCEEEEEEECHHHHHHCCCCCCCCEEEECC
ALGPFVRVDSCIYKDYVIPPFYDSMVAKLIVKASSYNLAVNKLSRALNEFTIKGVKTTIP
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FLINICNDKDFRRGYFDTSYIESKIKELMPPPQSKPEDDMVSVIVAALSARYGA
HHHHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFQKILIANRGEIAVRIIRACRDLHIKSIAIYARADRDCLHVKMADESYEISDDPLQGYL
CCCEEEEECCCHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
DADLIIEVALRAEADAIHPGYGFLSENATFAKKVQEAGITWIGPDALTIAKMGDKNAARA
CHHHHEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEECCCCHHHHH
IMEKNGIPIVPGTQPLNKHSISDIAKYAQKIGYPVILKASSGGGGRGIRVVEKEEDLIES
HHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHH
FEACKREAMAFFKNDDVFMEKYIVNPRHIEFQILADNYGNVIHLLERDCSIQRRHQKLVE
HHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEEEEECCCCCEEEHHHHCCHHHHHHHHHHH
IAPSPLISDDLRRRMGAAAVAAAKAAQYTNAGTVEFLLDENNTFYFMEMNTRIQVEHGVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCEEEEECCCC
EEITGYDLIGRQIRIAQGEILDLTQHDIRAQGFAIEVRINAEDVANDFVPNPGKITTYYP
CCCCCHHHCCCEEEECCCCEEECHHHHHCCCCEEEEEEECHHHHHHCCCCCCCCEEEECC
ALGPFVRVDSCIYKDYVIPPFYDSMVAKLIVKASSYNLAVNKLSRALNEFTIKGVKTTIP
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FLINICNDKDFRRGYFDTSYIESKIKELMPPPQSKPEDDMVSVIVAALSARYGA
HHHHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]