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The map label for this gene is nifS_2

Identifier: 32267238

GI number: 32267238

Start: 1663628

End: 1665055

Strand: Direct

Name: nifS_2

Synonym: HH1739

Alternate gene names: 32267238

Gene position: 1663628-1665055 (Clockwise)

Preceding gene: 32267237

Following gene: 32267240

Centisome position: 92.47

GC content: 35.5

Gene sequence:

>1428_bases
ATGGCTCTTTTGCATACGCATTCAAAGGTTGTTAAACTCATTCGTCATTTTTTTACACCTTTACTTGATAGTCGCTATGA
TAAGCTTTCGCAACTTCGTTCAAATACTATTTTGGATTCTAAACATTATTATTTTGACTTCACAGCTTCCGGTCTTGCTT
ATAAGATAATCAACAAACGTATAGAATCTATTTTACCCTATTATGCTAATACACATTCTTATATAGCCTCTAATGCTGCT
CTAATGAGCGGACTTTATGCAGAGGCAAAGGAGAGTATTTCCTATTCAATCGGGCTTAATAAAGATTTTATACTCATCGC
TGGGGGTAGTGGAGCGAGTTTTAGTATTAAAAAATTTCAAGAGATTATGGGGATTTATGCGCCTCCACGCAGTTTATCAC
ATTTACAACATTTAAATAAAAATCTTTCTTCCACGCCAAAACTTACTTTGCTTCAACAACTTAAAGATTATGATGCACAC
ACGCATACATTAAATTTCCCTCAAATTATTATAAGTGGGTTTGAACATCATTCTAATGAAATAAGTTACCGACAAGGGCT
TTGTCATATTCATAAAATTGCTTTTGATATAGAAGGATTACCTGATATAAAGCATTTAGAAGAGATTTTGCAAAAGATAA
AAAAAGAAAATAAGCACAGCACGATTATTGCTTCTTTAAGCGCAGCGTCTAATGTTACAGGGATTCTCACACCATTAGAA
TCTTGTATAGCATTACTTAGACGCTATGGAGCTATTATTGCGCTTGATATGGCAAGCTCATCGGCTTATATGCAAGTAGA
TTGTGCTGATTTTGATGTGGCATTTCTTGCACCGCATAAGCTTTTGGGTGGTGTAGGCGCGTGTGGAATCTTGGCAATAA
GAAAATCTTTACTTGATGCACAGATTCCTCCAAGTTTTGCTGGTGGAGGTGTGATTAAATATGCCAATGATACGACACAT
TGCTTCATTGATGATATAGCTTTGCGTGAAGAAGCGGGTACTCCACCTATTTTAGGCTTACTCTATGCAGCATTAGCCTA
TCAATTACGCAATGAAATAGGGTTAGAATGGATTAAACGAAGAGAGAATATTCTCACTCAAGCCTTTTTATATGAATTAC
AAAAAATCCCAGCTGTAAGTATTTATGGAGATTTATCAAAAGAAAGATTAGGTATAGTGAGCTTTAATATTGGTTCAATT
TCACCACTTGATTTATGTGCGCTTTTGAGTAAAAAATTTGGCATACAAACTCGTGCAGGGTGTAGTTGTGCGGGTCCTTA
TGGACATTATCTTATACCTGATGGTAGCTATGAGAAAAAGCCAATGTGGCTACGAGTAAGTATCCATTATACCCACACGA
TAAAGGATATAGAATATCTCATTGATGCAATTAAGACTTGTGTGAAAATATTAAGAGGCTCATTTTAG

Upstream 100 bases:

>100_bases
ATACACATATGTGCAAAAAGATACATAAAGTAACAGCTTGTAGAAGATTGCATAAAAAGGCAAAAATAAAATTTCATACA
ATTTCTTTAGAAGGAAAGTA

Downstream 100 bases:

>100_bases
ATTCTATAAGTTAAGCCTTTTTTTGGCTTTCCCTACTTCTCAAGTAGTGCATATAAAAGAATGGCTATAAACCCAAAGCC
TACAGGCTTTGGAGTAAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MALLHTHSKVVKLIRHFFTPLLDSRYDKLSQLRSNTILDSKHYYFDFTASGLAYKIINKRIESILPYYANTHSYIASNAA
LMSGLYAEAKESISYSIGLNKDFILIAGGSGASFSIKKFQEIMGIYAPPRSLSHLQHLNKNLSSTPKLTLLQQLKDYDAH
THTLNFPQIIISGFEHHSNEISYRQGLCHIHKIAFDIEGLPDIKHLEEILQKIKKENKHSTIIASLSAASNVTGILTPLE
SCIALLRRYGAIIALDMASSSAYMQVDCADFDVAFLAPHKLLGGVGACGILAIRKSLLDAQIPPSFAGGGVIKYANDTTH
CFIDDIALREEAGTPPILGLLYAALAYQLRNEIGLEWIKRRENILTQAFLYELQKIPAVSIYGDLSKERLGIVSFNIGSI
SPLDLCALLSKKFGIQTRAGCSCAGPYGHYLIPDGSYEKKPMWLRVSIHYTHTIKDIEYLIDAIKTCVKILRGSF

Sequences:

>Translated_475_residues
MALLHTHSKVVKLIRHFFTPLLDSRYDKLSQLRSNTILDSKHYYFDFTASGLAYKIINKRIESILPYYANTHSYIASNAA
LMSGLYAEAKESISYSIGLNKDFILIAGGSGASFSIKKFQEIMGIYAPPRSLSHLQHLNKNLSSTPKLTLLQQLKDYDAH
THTLNFPQIIISGFEHHSNEISYRQGLCHIHKIAFDIEGLPDIKHLEEILQKIKKENKHSTIIASLSAASNVTGILTPLE
SCIALLRRYGAIIALDMASSSAYMQVDCADFDVAFLAPHKLLGGVGACGILAIRKSLLDAQIPPSFAGGGVIKYANDTTH
CFIDDIALREEAGTPPILGLLYAALAYQLRNEIGLEWIKRRENILTQAFLYELQKIPAVSIYGDLSKERLGIVSFNIGSI
SPLDLCALLSKKFGIQTRAGCSCAGPYGHYLIPDGSYEKKPMWLRVSIHYTHTIKDIEYLIDAIKTCVKILRGSF
>Mature_474_residues
ALLHTHSKVVKLIRHFFTPLLDSRYDKLSQLRSNTILDSKHYYFDFTASGLAYKIINKRIESILPYYANTHSYIASNAAL
MSGLYAEAKESISYSIGLNKDFILIAGGSGASFSIKKFQEIMGIYAPPRSLSHLQHLNKNLSSTPKLTLLQQLKDYDAHT
HTLNFPQIIISGFEHHSNEISYRQGLCHIHKIAFDIEGLPDIKHLEEILQKIKKENKHSTIIASLSAASNVTGILTPLES
CIALLRRYGAIIALDMASSSAYMQVDCADFDVAFLAPHKLLGGVGACGILAIRKSLLDAQIPPSFAGGGVIKYANDTTHC
FIDDIALREEAGTPPILGLLYAALAYQLRNEIGLEWIKRRENILTQAFLYELQKIPAVSIYGDLSKERLGIVSFNIGSIS
PLDLCALLSKKFGIQTRAGCSCAGPYGHYLIPDGSYEKKPMWLRVSIHYTHTIKDIEYLIDAIKTCVKILRGSF

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=306, Percent_Identity=27.7777777777778, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789175, Length=314, Percent_Identity=26.7515923566879, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193211090, Length=469, Percent_Identity=26.865671641791, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 52753; Mature: 52622

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLHTHSKVVKLIRHFFTPLLDSRYDKLSQLRSNTILDSKHYYFDFTASGLAYKIINKR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEECCCHHHHHHHHH
IESILPYYANTHSYIASNAALMSGLYAEAKESISYSIGLNKDFILIAGGSGASFSIKKFQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCHHHHHH
EIMGIYAPPRSLSHLQHLNKNLSSTPKLTLLQQLKDYDAHTHTLNFPQIIISGFEHHSNE
HHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCC
ISYRQGLCHIHKIAFDIEGLPDIKHLEEILQKIKKENKHSTIIASLSAASNVTGILTPLE
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHH
SCIALLRRYGAIIALDMASSSAYMQVDCADFDVAFLAPHKLLGGVGACGILAIRKSLLDA
HHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCHHHCHHHHHCCCCHHHHHHHHHHHHHC
QIPPSFAGGGVIKYANDTTHCFIDDIALREEAGTPPILGLLYAALAYQLRNEIGLEWIKR
CCCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RENILTQAFLYELQKIPAVSIYGDLSKERLGIVSFNIGSISPLDLCALLSKKFGIQTRAG
HHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
CSCAGPYGHYLIPDGSYEKKPMWLRVSIHYTHTIKDIEYLIDAIKTCVKILRGSF
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALLHTHSKVVKLIRHFFTPLLDSRYDKLSQLRSNTILDSKHYYFDFTASGLAYKIINKR
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEECCCHHHHHHHHH
IESILPYYANTHSYIASNAALMSGLYAEAKESISYSIGLNKDFILIAGGSGASFSIKKFQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCHHHHHH
EIMGIYAPPRSLSHLQHLNKNLSSTPKLTLLQQLKDYDAHTHTLNFPQIIISGFEHHSNE
HHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCC
ISYRQGLCHIHKIAFDIEGLPDIKHLEEILQKIKKENKHSTIIASLSAASNVTGILTPLE
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHH
SCIALLRRYGAIIALDMASSSAYMQVDCADFDVAFLAPHKLLGGVGACGILAIRKSLLDA
HHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCHHHCHHHHHCCCCHHHHHHHHHHHHHC
QIPPSFAGGGVIKYANDTTHCFIDDIALREEAGTPPILGLLYAALAYQLRNEIGLEWIKR
CCCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RENILTQAFLYELQKIPAVSIYGDLSKERLGIVSFNIGSISPLDLCALLSKKFGIQTRAG
HHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
CSCAGPYGHYLIPDGSYEKKPMWLRVSIHYTHTIKDIEYLIDAIKTCVKILRGSF
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]