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The map label for this gene is nifS_1

Identifier: 32267186

GI number: 32267186

Start: 1613968

End: 1615164

Strand: Direct

Name: nifS_1

Synonym: HH1687

Alternate gene names: 32267186

Gene position: 1613968-1615164 (Clockwise)

Preceding gene: 32267185

Following gene: 32267188

Centisome position: 89.71

GC content: 39.18

Gene sequence:

>1197_bases
ATGTGGCAATTATGCTTCTTGGATAACAATAAGCTTATGAGAGATATGAATACATTGATTTATCTTGACAATGCCGCTAC
TTCCTTTCCTAAGCCATTGTGTGTAATTGAGGCAGTGAAAGATTATCTTTGTAATGTGGGGGCTTCTCCTGCTAGGAGTG
CGCATTCTCTTTCTATAGAATCTGGGCGGATTCTCTATGAGGCGCGTGTAGGCTTGGCAGAGCTACTTCATCAGCCAAAT
GAGGAGCGTATCATATTTGGTGCAAATGCTACAACTATGCTTAATAGTGCACTTTATGGTTTGCTTAAAAAAGGCGATAA
GGTGCTTACTACGACATTAGAACATAATAGTGTTTTGCGTCCGCTTGAAAACTTAAGGCTAAAATATGGTATTACGCTTG
AGTTTATTCAATCTTCACAAACTTGTAAGCTTGATTTATGCGATATTGAATCTAAGCTCAAAGGAGCAAAAGCTTTTGTA
TGTGTATATGCGAATAATATAACAGGCGCAGTATTACCTATTGAAGAAATTTATAAGCTTTGCATAAAGCATAAAGTGAT
ATTTATCCTTGATGCTTCCCAAGCTGTGGGTTATTTACCCTTAAAGGCTACGAGTGCAGATATTATTTGTGCCTCTTGTC
ATAAGGGCTTATATGCACCAAGTGCGCTTGGGTTTATGAGTTTAAATCCTCATTTTAATGAGGAACTTTTAGAGAGTTTT
ATGCAAGGGGGCAGTGGCTCATTGAGTGAGGAAATCACACAACCTTGCATATTACCTGATAAATATGAGAGCGGCACACC
TAATATGTGTGCGATTGCTGGGCTTAGAGCTGGGCTTGAATGGATAAAGGAGCAGGATATAGAGCAAATCCATACCCACA
AGATGAAATTGCGCCAATATCTCTATGATGGGCTTAAATCAATCCAGCGTGCAAAGATTTATGAGATAGAAAGTAAGTGG
TGTGTTGGGAATCTTGCCTTTAATATTGAGGGTATGTATCCCTCAAAAGTAGGATTGAGACTTGATAGGGAATTTGGGAT
TCTCACACGCGTGGGATTACATTGCTCACCACTTACTCATAAAAGCATAGGAAGCTTTTCTTGTGGAGGAAGCATTCGTT
TAAGTGCTGGGGTATTTAATACAACTGAAGAGATCGAGCACACTTTAGAAGCGATTAAAATCATTGCTAGGACTTAG

Upstream 100 bases:

>100_bases
GCCATTGTAGAAGCTATGAAAATGGAGAATGAGGTGCTTTCTCCTATTGATGGTGTAGTTAAAGAAATTTATGCAACTCA
AGGAGCGCAAATAGGGAGCG

Downstream 100 bases:

>100_bases
TTTTGTAGATAATCTTGCAAGGCATAAGCTTCATTCATATTGCTATTTTGCATTTCTTTAATGGCTTCAATGGCTACACG
CGCTGCTGATAGCGTAGTAA

Product: NifS family aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MWQLCFLDNNKLMRDMNTLIYLDNAATSFPKPLCVIEAVKDYLCNVGASPARSAHSLSIESGRILYEARVGLAELLHQPN
EERIIFGANATTMLNSALYGLLKKGDKVLTTTLEHNSVLRPLENLRLKYGITLEFIQSSQTCKLDLCDIESKLKGAKAFV
CVYANNITGAVLPIEEIYKLCIKHKVIFILDASQAVGYLPLKATSADIICASCHKGLYAPSALGFMSLNPHFNEELLESF
MQGGSGSLSEEITQPCILPDKYESGTPNMCAIAGLRAGLEWIKEQDIEQIHTHKMKLRQYLYDGLKSIQRAKIYEIESKW
CVGNLAFNIEGMYPSKVGLRLDREFGILTRVGLHCSPLTHKSIGSFSCGGSIRLSAGVFNTTEEIEHTLEAIKIIART

Sequences:

>Translated_398_residues
MWQLCFLDNNKLMRDMNTLIYLDNAATSFPKPLCVIEAVKDYLCNVGASPARSAHSLSIESGRILYEARVGLAELLHQPN
EERIIFGANATTMLNSALYGLLKKGDKVLTTTLEHNSVLRPLENLRLKYGITLEFIQSSQTCKLDLCDIESKLKGAKAFV
CVYANNITGAVLPIEEIYKLCIKHKVIFILDASQAVGYLPLKATSADIICASCHKGLYAPSALGFMSLNPHFNEELLESF
MQGGSGSLSEEITQPCILPDKYESGTPNMCAIAGLRAGLEWIKEQDIEQIHTHKMKLRQYLYDGLKSIQRAKIYEIESKW
CVGNLAFNIEGMYPSKVGLRLDREFGILTRVGLHCSPLTHKSIGSFSCGGSIRLSAGVFNTTEEIEHTLEAIKIIART
>Mature_398_residues
MWQLCFLDNNKLMRDMNTLIYLDNAATSFPKPLCVIEAVKDYLCNVGASPARSAHSLSIESGRILYEARVGLAELLHQPN
EERIIFGANATTMLNSALYGLLKKGDKVLTTTLEHNSVLRPLENLRLKYGITLEFIQSSQTCKLDLCDIESKLKGAKAFV
CVYANNITGAVLPIEEIYKLCIKHKVIFILDASQAVGYLPLKATSADIICASCHKGLYAPSALGFMSLNPHFNEELLESF
MQGGSGSLSEEITQPCILPDKYESGTPNMCAIAGLRAGLEWIKEQDIEQIHTHKMKLRQYLYDGLKSIQRAKIYEIESKW
CVGNLAFNIEGMYPSKVGLRLDREFGILTRVGLHCSPLTHKSIGSFSCGGSIRLSAGVFNTTEEIEHTLEAIKIIART

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=391, Percent_Identity=28.1329923273657, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=26.75, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=27.0886075949367, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI48994898, Length=400, Percent_Identity=26.75, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=397, Percent_Identity=27.2040302267003, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=392, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129463, Length=237, Percent_Identity=32.0675105485232, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44117; Mature: 44117

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQLCFLDNNKLMRDMNTLIYLDNAATSFPKPLCVIEAVKDYLCNVGASPARSAHSLSIE
CEEEEEECCCHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEC
SGRILYEARVGLAELLHQPNEERIIFGANATTMLNSALYGLLKKGDKVLTTTLEHNSVLR
CCCEEEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
PLENLRLKYGITLEFIQSSQTCKLDLCDIESKLKGAKAFVCVYANNITGAVLPIEEIYKL
HHHHCEEEECEEEEEECCCCCEEEEEECCHHHCCCCEEEEEEEECCCCCEEECHHHHHHH
CIKHKVIFILDASQAVGYLPLKATSADIICASCHKGLYAPSALGFMSLNPHFNEELLESF
HHHCCEEEEEECCCCCEEEEEECCCCCEEEEHHCCCCCCCCCCCEEECCCCCCHHHHHHH
MQGGSGSLSEEITQPCILPDKYESGTPNMCAIAGLRAGLEWIKEQDIEQIHTHKMKLRQY
HCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYDGLKSIQRAKIYEIESKWCVGNLAFNIEGMYPSKVGLRLDREFGILTRVGLHCSPLTH
HHHHHHHHHHHHEEEECCCEEEEEEEEEEECCCCCCCCEEECCCCCHHHHCCCCCCCCCC
KSIGSFSCGGSIRLSAGVFNTTEEIEHTLEAIKIIART
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWQLCFLDNNKLMRDMNTLIYLDNAATSFPKPLCVIEAVKDYLCNVGASPARSAHSLSIE
CEEEEEECCCHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEC
SGRILYEARVGLAELLHQPNEERIIFGANATTMLNSALYGLLKKGDKVLTTTLEHNSVLR
CCCEEEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
PLENLRLKYGITLEFIQSSQTCKLDLCDIESKLKGAKAFVCVYANNITGAVLPIEEIYKL
HHHHCEEEECEEEEEECCCCCEEEEEECCHHHCCCCEEEEEEEECCCCCEEECHHHHHHH
CIKHKVIFILDASQAVGYLPLKATSADIICASCHKGLYAPSALGFMSLNPHFNEELLESF
HHHCCEEEEEECCCCCEEEEEECCCCCEEEEHHCCCCCCCCCCCEEECCCCCCHHHHHHH
MQGGSGSLSEEITQPCILPDKYESGTPNMCAIAGLRAGLEWIKEQDIEQIHTHKMKLRQY
HCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYDGLKSIQRAKIYEIESKWCVGNLAFNIEGMYPSKVGLRLDREFGILTRVGLHCSPLTH
HHHHHHHHHHHHEEEECCCEEEEEEEEEEECCCCCCCCEEECCCCCHHHHCCCCCCCCCC
KSIGSFSCGGSIRLSAGVFNTTEEIEHTLEAIKIIART
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]