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The map label for this gene is pycB

Identifier: 32267185

GI number: 32267185

Start: 1612194

End: 1613993

Strand: Direct

Name: pycB

Synonym: HH1686

Alternate gene names: 32267185

Gene position: 1612194-1613993 (Clockwise)

Preceding gene: 32267184

Following gene: 32267186

Centisome position: 89.61

GC content: 40.06

Gene sequence:

>1800_bases
ATGATAAAAATCACAGAAAATAGTTTGCGAGATGGACACCAGTCGCTTTTGGCTACTCGTATGCGCACGCAAGATTTGCT
TGAGGCAGCAAAGATTTTTGATTCTATTGGCTTTCATAGCGTAGAAGTATGGGGTGGGGCAACTTATGATACTTGCTTAC
GCTATCTTAAAGAAGACCCTTTTGAACGTTTGAATGAGTTTAAAAAAGTTTTTGTAAAAACGCCATTGCAAATGCTTCTA
AGAGGACAGAATCTTATAGGCTATCGGCATTATGCTGATGATGTGGTTGAGGAATTTGTAAGGCTTTCAAGTGAAGCAGG
TATGGATATTTTTAGAATCTTTGATGCCTTTAATGATGCTCGTAATCTTAGAGTTTCTATTGAGAGCGTGAAAAAATATG
GCAAACACGCACAAGGTGCAATTTGTTATACAACTTCTCCTGTGCATACAATTTCAAGCTTTGTAGATTATGCAAAAGAG
CTTGTAGAAATGGGTTGTGATTCTATTGCAATTAAGGATATGGCGGGTTTGCTTACGCCATTTACAGCGTATGATTTGGT
AAAAGCACTCAAAGAAGAGCTAGGTGTATATCTATGTTTACATACGCACTCAACCGCAGGGTTTGCATTTGGAGCACATT
TGAAAGCAGTAGAGGCTGGAGTTGATGTGTTAGATTTAGCAAATTCAGCTTTAAGTGAAGGCACAAGCCACCCTTGCACA
CAATCTATGGTCGCTACACTTAGAGGGACAAAATGGGATACTAAGCTTGATATAGCCTCTATGGAGATAGCAGCAGAGAT
TTTGCGCAAAAACCGCCGTAAATATAAAAAATTTGAATCTCAATATAATCAAATTGATACGCGTGTGCTTGTCAATCAGA
TTCCAGGTGGTATGATTTCAAATATGGCAAATCAACTCAAAGAACAAAATGCTCTTGATAGAATGGAAGAAGTAATGCAG
GAGATTCCAAATGTGCGGGCGGATTTTGGTTATGTGCCTTTGGTAACACCTTCAAGTCAGATTGTTGGCACTCAAGCAGT
GCTTAATGTGCTTATGGGAGAGCGATATAAGACTATTACAACGGAAACGCGCAACGTAATTAAGGGGCTATATGGTCGCA
CACCAGCACCTTTAAATGAAATGTTAGTAAAAAAAGTTTTAAGCGAGGGTGAAGATATTGTTCGTGTGCGCCCTGCGGAT
TTACTCTCTCCTGAACTTAAAAAAGCAAAAGAAGAAAGTGCAGGATTTGCTAAGAGTGCGCAAGATGTTATATCTTATGC
AATGTTTGGGACTATTGCGCAAACGTTTTTGCAAGAGCGCAATGCTAATGCGCTTAACCCCGAGCCTTTGGAATCATATG
AAGAAAAATGTGATGATAAATTACCTAAAGAGTTTGAGATTGTTGTCAATGGAGAGCACTATGCGATTAAAATTGAGGGG
AGTGGCGAAAAAACAGATGAGGTGCGTCCATTTTTTATACGTGTTGATGGGGAGCTTAAGGAGGTATTTGTTGAGAGTAT
TTCCACTCCTTTGGGGGGAAAGGTTATCAAGGAGCAAAAAGCAGGTGCTTTGCCTCAAGCAACTGCACCCGGCCACGCTA
AATCTCCGATGCCCGGCACACTTACAAAACTTAAAGTGAAAGTAGGCGATAAAGTTAAACAAGGCGATACTCTAGCCATT
GTAGAAGCTATGAAAATGGAGAATGAGGTGCTTTCTCCTATTGATGGTGTAGTTAAAGAAATTTATGCAACTCAAGGAGC
GCAAATAGGGAGCGATGTGGCAATTATGCTTCTTGGATAA

Upstream 100 bases:

>100_bases
ATTAACCTATGAGGTGCTCTCTTAGTCTGTATTTTGTCATTTATAATGCCAAATGCGATAGAATTTATAATAATAAACAA
TCCAAATAGAAGGTGAGGTT

Downstream 100 bases:

>100_bases
CAATAAGCTTATGAGAGATATGAATACATTGATTTATCTTGACAATGCCGCTACTTCCTTTCCTAAGCCATTGTGTGTAA
TTGAGGCAGTGAAAGATTAT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MIKITENSLRDGHQSLLATRMRTQDLLEAAKIFDSIGFHSVEVWGGATYDTCLRYLKEDPFERLNEFKKVFVKTPLQMLL
RGQNLIGYRHYADDVVEEFVRLSSEAGMDIFRIFDAFNDARNLRVSIESVKKYGKHAQGAICYTTSPVHTISSFVDYAKE
LVEMGCDSIAIKDMAGLLTPFTAYDLVKALKEELGVYLCLHTHSTAGFAFGAHLKAVEAGVDVLDLANSALSEGTSHPCT
QSMVATLRGTKWDTKLDIASMEIAAEILRKNRRKYKKFESQYNQIDTRVLVNQIPGGMISNMANQLKEQNALDRMEEVMQ
EIPNVRADFGYVPLVTPSSQIVGTQAVLNVLMGERYKTITTETRNVIKGLYGRTPAPLNEMLVKKVLSEGEDIVRVRPAD
LLSPELKKAKEESAGFAKSAQDVISYAMFGTIAQTFLQERNANALNPEPLESYEEKCDDKLPKEFEIVVNGEHYAIKIEG
SGEKTDEVRPFFIRVDGELKEVFVESISTPLGGKVIKEQKAGALPQATAPGHAKSPMPGTLTKLKVKVGDKVKQGDTLAI
VEAMKMENEVLSPIDGVVKEIYATQGAQIGSDVAIMLLG

Sequences:

>Translated_599_residues
MIKITENSLRDGHQSLLATRMRTQDLLEAAKIFDSIGFHSVEVWGGATYDTCLRYLKEDPFERLNEFKKVFVKTPLQMLL
RGQNLIGYRHYADDVVEEFVRLSSEAGMDIFRIFDAFNDARNLRVSIESVKKYGKHAQGAICYTTSPVHTISSFVDYAKE
LVEMGCDSIAIKDMAGLLTPFTAYDLVKALKEELGVYLCLHTHSTAGFAFGAHLKAVEAGVDVLDLANSALSEGTSHPCT
QSMVATLRGTKWDTKLDIASMEIAAEILRKNRRKYKKFESQYNQIDTRVLVNQIPGGMISNMANQLKEQNALDRMEEVMQ
EIPNVRADFGYVPLVTPSSQIVGTQAVLNVLMGERYKTITTETRNVIKGLYGRTPAPLNEMLVKKVLSEGEDIVRVRPAD
LLSPELKKAKEESAGFAKSAQDVISYAMFGTIAQTFLQERNANALNPEPLESYEEKCDDKLPKEFEIVVNGEHYAIKIEG
SGEKTDEVRPFFIRVDGELKEVFVESISTPLGGKVIKEQKAGALPQATAPGHAKSPMPGTLTKLKVKVGDKVKQGDTLAI
VEAMKMENEVLSPIDGVVKEIYATQGAQIGSDVAIMLLG
>Mature_599_residues
MIKITENSLRDGHQSLLATRMRTQDLLEAAKIFDSIGFHSVEVWGGATYDTCLRYLKEDPFERLNEFKKVFVKTPLQMLL
RGQNLIGYRHYADDVVEEFVRLSSEAGMDIFRIFDAFNDARNLRVSIESVKKYGKHAQGAICYTTSPVHTISSFVDYAKE
LVEMGCDSIAIKDMAGLLTPFTAYDLVKALKEELGVYLCLHTHSTAGFAFGAHLKAVEAGVDVLDLANSALSEGTSHPCT
QSMVATLRGTKWDTKLDIASMEIAAEILRKNRRKYKKFESQYNQIDTRVLVNQIPGGMISNMANQLKEQNALDRMEEVMQ
EIPNVRADFGYVPLVTPSSQIVGTQAVLNVLMGERYKTITTETRNVIKGLYGRTPAPLNEMLVKKVLSEGEDIVRVRPAD
LLSPELKKAKEESAGFAKSAQDVISYAMFGTIAQTFLQERNANALNPEPLESYEEKCDDKLPKEFEIVVNGEHYAIKIEG
SGEKTDEVRPFFIRVDGELKEVFVESISTPLGGKVIKEQKAGALPQATAPGHAKSPMPGTLTKLKVKVGDKVKQGDTLAI
VEAMKMENEVLSPIDGVVKEIYATQGAQIGSDVAIMLLG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=624, Percent_Identity=31.8910256410256, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI106049295, Length=624, Percent_Identity=31.8910256410256, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI106049292, Length=624, Percent_Identity=31.8910256410256, Blast_Score=268, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17562816, Length=619, Percent_Identity=33.7641357027464, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6321376, Length=620, Percent_Identity=30.1612903225806, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319695, Length=623, Percent_Identity=29.5345104333868, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24652212, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24652210, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24652214, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI19921944, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24652216, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI281363050, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652224, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652222, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652220, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652218, Length=633, Percent_Identity=32.7014218009479, Blast_Score=294, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 66307; Mature: 66307

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITENSLRDGHQSLLATRMRTQDLLEAAKIFDSIGFHSVEVWGGATYDTCLRYLKEDP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCH
FERLNEFKKVFVKTPLQMLLRGQNLIGYRHYADDVVEEFVRLSSEAGMDIFRIFDAFNDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCH
RNLRVSIESVKKYGKHAQGAICYTTSPVHTISSFVDYAKELVEMGCDSIAIKDMAGLLTP
HHHEEEHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCH
FTAYDLVKALKEELGVYLCLHTHSTAGFAFGAHLKAVEAGVDVLDLANSALSEGTSHPCT
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
QSMVATLRGTKWDTKLDIASMEIAAEILRKNRRKYKKFESQYNQIDTRVLVNQIPGGMIS
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
NMANQLKEQNALDRMEEVMQEIPNVRADFGYVPLVTPSSQIVGTQAVLNVLMGERYKTIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHH
TETRNVIKGLYGRTPAPLNEMLVKKVLSEGEDIVRVRPADLLSPELKKAKEESAGFAKSA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHCCHHHHH
QDVISYAMFGTIAQTFLQERNANALNPEPLESYEEKCDDKLPKEFEIVVNGEHYAIKIEG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
SGEKTDEVRPFFIRVDGELKEVFVESISTPLGGKVIKEQKAGALPQATAPGHAKSPMPGT
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
LTKLKVKVGDKVKQGDTLAIVEAMKMENEVLSPIDGVVKEIYATQGAQIGSDVAIMLLG
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure
MIKITENSLRDGHQSLLATRMRTQDLLEAAKIFDSIGFHSVEVWGGATYDTCLRYLKEDP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCH
FERLNEFKKVFVKTPLQMLLRGQNLIGYRHYADDVVEEFVRLSSEAGMDIFRIFDAFNDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCH
RNLRVSIESVKKYGKHAQGAICYTTSPVHTISSFVDYAKELVEMGCDSIAIKDMAGLLTP
HHHEEEHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCH
FTAYDLVKALKEELGVYLCLHTHSTAGFAFGAHLKAVEAGVDVLDLANSALSEGTSHPCT
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
QSMVATLRGTKWDTKLDIASMEIAAEILRKNRRKYKKFESQYNQIDTRVLVNQIPGGMIS
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
NMANQLKEQNALDRMEEVMQEIPNVRADFGYVPLVTPSSQIVGTQAVLNVLMGERYKTIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHH
TETRNVIKGLYGRTPAPLNEMLVKKVLSEGEDIVRVRPADLLSPELKKAKEESAGFAKSA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHCCHHHHH
QDVISYAMFGTIAQTFLQERNANALNPEPLESYEEKCDDKLPKEFEIVVNGEHYAIKIEG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
SGEKTDEVRPFFIRVDGELKEVFVESISTPLGGKVIKEQKAGALPQATAPGHAKSPMPGT
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCC
LTKLKVKVGDKVKQGDTLAIVEAMKMENEVLSPIDGVVKEIYATQGAQIGSDVAIMLLG
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]