Click here to switch to the map view.

The map label for this gene is lepB

Identifier: 32266866

GI number: 32266866

Start: 1321918

End: 1322931

Strand: Direct

Name: lepB

Synonym: HH1367

Alternate gene names: 32266866

Gene position: 1321918-1322931 (Clockwise)

Preceding gene: 32266865

Following gene: 32266867

Centisome position: 73.47

GC content: 36.19

Gene sequence:

>1014_bases
ATGAAAAAGTTTTTAAGTGCATTATGGGCATTTGCTTCAAGCTGGACAGGGACGATTGTTATCGTGCTTATTTTAATATT
TTTTGTTATGCAAGCGTTTGTTATTCCTACACGTTCAATGGTTGGCACACTTTTTGAGGGGGATTTTTTATTTGTTAAAA
AATTTAGTTATGGGATTCCCACACCACGGATTCCGTGGATTGAAGTGAATGTTTTACCTGATTTTTCTGGAAATGGACAC
TTGATTGCAGGTGAGCGTCCCAAGAGAGGTGATGTAGTAGTGTTTCTTCCTCCACATATAGAAAAAACTTATTTTGTTAA
ACGCACTTTTGCAGTAGGCGGTGATGAAGTGATTTTTGATAAAGATGGCTTATATCTCCACCCATTTGAGGGAGATGAAT
ATGTAAGAGAACATTATTCAGGGCAAATTATCGTAGAACGATTAGGCAAACTTTTTGTAAAAGACCCTTATCGTGGGCAG
CACCCCGGTATCACCTACCAAAGTGTATTTTATAAGAATCCAATGAGTATGGAGCGCTATTTTGCAGATGAAAATGGCAA
ACTTTATCAAAGAGTATGTGTTGAGGGCAATCAAGGGATAAATATAGGCGATGGTGAGGAAAGTTGCGAACTATGGCATA
AAGAATACACTTCAGAACCATTAGAATCTTTTATAAAAGATTCTAATATTGCTTATACAGATATGAGCAATGGACGAATG
ATAAGTTCTATGGTCAAGCTTGATGGTATGTATTATAAAAAAATTGATGATGATGAGTTTTTTATGGTAGGAGATAATCG
CAACAACAGCTTTGATTCTAGATTTTGGGGAAGTGTGCCTTATAAAAATATTATTGGTAAGCCTTGGTTTATATATTTTA
GTATTAATAAGGCAAATTCTCAAGAAGCAGGAGCCGATGAAGATAAAAGCAAGCGTTATACAATTCGTTGGGAGCGAATG
TTTAAAACAACAGAGGGACTTGAAAAAGCTGCTCGAGAGGCAATGCCAAAATGA

Upstream 100 bases:

>100_bases
TTACACCTGTGCCCGGTGGTGTAGGTCCTATGACTATTGTTAGCCTACTTCAAAATACTTATAAGGCTGCAATGTATCGT
TTAGAGCAAAGGATGCGAGT

Downstream 100 bases:

>100_bases
AAAGAGATACAATGACTTATGTTATAGGGACAGACCACGCAGGATTTAAGCTCAAGGAATTTACTATTTCATTTTTGCAG
AAAAAAGGTTTTGAAATCAT

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKKFLSALWAFASSWTGTIVIVLILIFFVMQAFVIPTRSMVGTLFEGDFLFVKKFSYGIPTPRIPWIEVNVLPDFSGNGH
LIAGERPKRGDVVVFLPPHIEKTYFVKRTFAVGGDEVIFDKDGLYLHPFEGDEYVREHYSGQIIVERLGKLFVKDPYRGQ
HPGITYQSVFYKNPMSMERYFADENGKLYQRVCVEGNQGINIGDGEESCELWHKEYTSEPLESFIKDSNIAYTDMSNGRM
ISSMVKLDGMYYKKIDDDEFFMVGDNRNNSFDSRFWGSVPYKNIIGKPWFIYFSINKANSQEAGADEDKSKRYTIRWERM
FKTTEGLEKAAREAMPK

Sequences:

>Translated_337_residues
MKKFLSALWAFASSWTGTIVIVLILIFFVMQAFVIPTRSMVGTLFEGDFLFVKKFSYGIPTPRIPWIEVNVLPDFSGNGH
LIAGERPKRGDVVVFLPPHIEKTYFVKRTFAVGGDEVIFDKDGLYLHPFEGDEYVREHYSGQIIVERLGKLFVKDPYRGQ
HPGITYQSVFYKNPMSMERYFADENGKLYQRVCVEGNQGINIGDGEESCELWHKEYTSEPLESFIKDSNIAYTDMSNGRM
ISSMVKLDGMYYKKIDDDEFFMVGDNRNNSFDSRFWGSVPYKNIIGKPWFIYFSINKANSQEAGADEDKSKRYTIRWERM
FKTTEGLEKAAREAMPK
>Mature_337_residues
MKKFLSALWAFASSWTGTIVIVLILIFFVMQAFVIPTRSMVGTLFEGDFLFVKKFSYGIPTPRIPWIEVNVLPDFSGNGH
LIAGERPKRGDVVVFLPPHIEKTYFVKRTFAVGGDEVIFDKDGLYLHPFEGDEYVREHYSGQIIVERLGKLFVKDPYRGQ
HPGITYQSVFYKNPMSMERYFADENGKLYQRVCVEGNQGINIGDGEESCELWHKEYTSEPLESFIKDSNIAYTDMSNGRM
ISSMVKLDGMYYKKIDDDEFFMVGDNRNNSFDSRFWGSVPYKNIIGKPWFIYFSINKANSQEAGADEDKSKRYTIRWERM
FKTTEGLEKAAREAMPK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=296, Percent_Identity=29.0540540540541, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 38932; Mature: 38932

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLSALWAFASSWTGTIVIVLILIFFVMQAFVIPTRSMVGTLFEGDFLFVKKFSYGIP
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCC
TPRIPWIEVNVLPDFSGNGHLIAGERPKRGDVVVFLPPHIEKTYFVKRTFAVGGDEVIFD
CCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEE
KDGLYLHPFEGDEYVREHYSGQIIVERLGKLFVKDPYRGQHPGITYQSVFYKNPMSMERY
CCCCEEECCCCHHHHHHHCCCEEHHHHHHHHEECCCCCCCCCCCEEHHEEECCCCCHHHH
FADENGKLYQRVCVEGNQGINIGDGEESCELWHKEYTSEPLESFIKDSNIAYTDMSNGRM
HCCCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCCCHH
ISSMVKLDGMYYKKIDDDEFFMVGDNRNNSFDSRFWGSVPYKNIIGKPWFIYFSINKANS
HHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCC
QEAGADEDKSKRYTIRWERMFKTTEGLEKAAREAMPK
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKFLSALWAFASSWTGTIVIVLILIFFVMQAFVIPTRSMVGTLFEGDFLFVKKFSYGIP
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCC
TPRIPWIEVNVLPDFSGNGHLIAGERPKRGDVVVFLPPHIEKTYFVKRTFAVGGDEVIFD
CCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEE
KDGLYLHPFEGDEYVREHYSGQIIVERLGKLFVKDPYRGQHPGITYQSVFYKNPMSMERY
CCCCEEECCCCHHHHHHHCCCEEHHHHHHHHEECCCCCCCCCCCEEHHEEECCCCCHHHH
FADENGKLYQRVCVEGNQGINIGDGEESCELWHKEYTSEPLESFIKDSNIAYTDMSNGRM
HCCCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCCCHH
ISSMVKLDGMYYKKIDDDEFFMVGDNRNNSFDSRFWGSVPYKNIIGKPWFIYFSINKANS
HHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCC
QEAGADEDKSKRYTIRWERMFKTTEGLEKAAREAMPK
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]