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The map label for this gene is folD

Identifier: 32266865

GI number: 32266865

Start: 1321061

End: 1321921

Strand: Direct

Name: folD

Synonym: HH1366

Alternate gene names: 32266865

Gene position: 1321061-1321921 (Clockwise)

Preceding gene: 32266863

Following gene: 32266866

Centisome position: 73.43

GC content: 39.61

Gene sequence:

>861_bases
ATGACATTATTAGATGGCAAATCCTTGAGTGTAACGATTGAGCGTGATTTAACAGAGCAAATTGCTGCACTTGTTTCTCA
AGGTGTAACGCCGGGTTTAGCAGTAATTTTGGTTGGCAATAATCCTGCAAGTTGCGCGTATGTGCAGATGAAAGCAAAGG
CGTGTAAGCGCGTAGGAATATACTCTGTTACCCACGAAATGCCTTCAAAAATTACACAAGATGAGCTTTTATCTGTTATT
GAGATTTTAAATAATGACCCAAATATTGATGGTATACTCGTTCAGCTGCCTTTGCCTGCACACATTCAGACAAGTAAAGT
GCTTGAAGCAATCAACCCTAAAAAAGATGTTGATGGTTTTCATCCATTTAATATAGGGCGATTGAGTGTGAATGTTGATA
CTTTTGTCCCTGCAACGCCACTTGGTGTAATGAAACTCCTTGAATCTTATCATATTGATGTCCGCGGTAAAGATGTTGTG
ATTATAGGTATGAGTAATATTGTGGGTAAGCCGCTTATGAGCCTTATGCTTAATGCAGGAGCAAGTGTAAGTTGTTGTCA
TATTCTCACGCGCGATGTGAAACAATATACTAAGAATGCGGATATTGTATGTGTGGCTGTGGGAAAAGTCGGATTACTTA
GTGCAGATATGATTAAAGAGGGTGCAGTTGTAGTAGATGTTGGTATTAATCGTTTAGAATCTGGCACACTTGTAGGAGAT
GTGGATTTTGAGAATGTCGCACCTAAGAGTAGTTTTATTACACCTGTGCCCGGTGGTGTAGGTCCTATGACTATTGTTAG
CCTACTTCAAAATACTTATAAGGCTGCAATGTATCGTTTAGAGCAAAGGATGCGAGTATGA

Upstream 100 bases:

>100_bases
AGAAACAATATCAGCTTTTTATTCATAAATCCTCACTTAAACGATATTTCTTTAATATCTTATATTATAATAACACAATG
ATAATCACAAAGGCTTTAAA

Downstream 100 bases:

>100_bases
AAAAGTTTTTAAGTGCATTATGGGCATTTGCTTCAAGCTGGACAGGGACGATTGTTATCGTGCTTATTTTAATATTTTTT
GTTATGCAAGCGTTTGTTAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTLLDGKSLSVTIERDLTEQIAALVSQGVTPGLAVILVGNNPASCAYVQMKAKACKRVGIYSVTHEMPSKITQDELLSVI
EILNNDPNIDGILVQLPLPAHIQTSKVLEAINPKKDVDGFHPFNIGRLSVNVDTFVPATPLGVMKLLESYHIDVRGKDVV
IIGMSNIVGKPLMSLMLNAGASVSCCHILTRDVKQYTKNADIVCVAVGKVGLLSADMIKEGAVVVDVGINRLESGTLVGD
VDFENVAPKSSFITPVPGGVGPMTIVSLLQNTYKAAMYRLEQRMRV

Sequences:

>Translated_286_residues
MTLLDGKSLSVTIERDLTEQIAALVSQGVTPGLAVILVGNNPASCAYVQMKAKACKRVGIYSVTHEMPSKITQDELLSVI
EILNNDPNIDGILVQLPLPAHIQTSKVLEAINPKKDVDGFHPFNIGRLSVNVDTFVPATPLGVMKLLESYHIDVRGKDVV
IIGMSNIVGKPLMSLMLNAGASVSCCHILTRDVKQYTKNADIVCVAVGKVGLLSADMIKEGAVVVDVGINRLESGTLVGD
VDFENVAPKSSFITPVPGGVGPMTIVSLLQNTYKAAMYRLEQRMRV
>Mature_285_residues
TLLDGKSLSVTIERDLTEQIAALVSQGVTPGLAVILVGNNPASCAYVQMKAKACKRVGIYSVTHEMPSKITQDELLSVIE
ILNNDPNIDGILVQLPLPAHIQTSKVLEAINPKKDVDGFHPFNIGRLSVNVDTFVPATPLGVMKLLESYHIDVRGKDVVI
IGMSNIVGKPLMSLMLNAGASVSCCHILTRDVKQYTKNADIVCVAVGKVGLLSADMIKEGAVVVDVGINRLESGTLVGDV
DFENVAPKSSFITPVPGGVGPMTIVSLLQNTYKAAMYRLEQRMRV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=299, Percent_Identity=45.4849498327759, Blast_Score=227, Evalue=7e-60,
Organism=Homo sapiens, GI222418558, Length=290, Percent_Identity=43.7931034482759, Blast_Score=220, Evalue=9e-58,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=38.3561643835616, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=50.3623188405797, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=41.2451361867704, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.4444444444444, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=37.8737541528239, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=25.6944444444444, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=43.2885906040268, Blast_Score=243, Evalue=8e-65,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=43.2885906040268, Blast_Score=243, Evalue=9e-65,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=37.4570446735395, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=37.4570446735395, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=37.4570446735395, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=37.4570446735395, Blast_Score=207, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_HELHP (Q7VGF6)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860897.1
- HSSP:   P11586
- ProteinModelPortal:   Q7VGF6
- SMR:   Q7VGF6
- GeneID:   1493054
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1366
- NMPDR:   fig|235279.1.peg.1366
- HOGENOM:   HBG328751
- OMA:   ELNADER
- ProtClustDB:   PRK14191
- BioCyc:   HHEP235279:HH_1366-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30647; Mature: 30516

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLDGKSLSVTIERDLTEQIAALVSQGVTPGLAVILVGNNPASCAYVQMKAKACKRVGI
CCEECCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCCH
YSVTHEMPSKITQDELLSVIEILNNDPNIDGILVQLPLPAHIQTSKVLEAINPKKDVDGF
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCC
HPFNIGRLSVNVDTFVPATPLGVMKLLESYHIDVRGKDVVIIGMSNIVGKPLMSLMLNAG
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCC
ASVSCCHILTRDVKQYTKNADIVCVAVGKVGLLSADMIKEGAVVVDVGINRLESGTLVGD
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEC
VDFENVAPKSSFITPVPGGVGPMTIVSLLQNTYKAAMYRLEQRMRV
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLLDGKSLSVTIERDLTEQIAALVSQGVTPGLAVILVGNNPASCAYVQMKAKACKRVGI
CEECCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCCH
YSVTHEMPSKITQDELLSVIEILNNDPNIDGILVQLPLPAHIQTSKVLEAINPKKDVDGF
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCC
HPFNIGRLSVNVDTFVPATPLGVMKLLESYHIDVRGKDVVIIGMSNIVGKPLMSLMLNAG
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCC
ASVSCCHILTRDVKQYTKNADIVCVAVGKVGLLSADMIKEGAVVVDVGINRLESGTLVGD
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEC
VDFENVAPKSSFITPVPGGVGPMTIVSLLQNTYKAAMYRLEQRMRV
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954