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The map label for this gene is minD

Identifier: 32266722

GI number: 32266722

Start: 1191873

End: 1192688

Strand: Direct

Name: minD

Synonym: HH1223

Alternate gene names: 32266722

Gene position: 1191873-1192688 (Clockwise)

Preceding gene: 32266721

Following gene: 32266723

Centisome position: 66.25

GC content: 39.58

Gene sequence:

>816_bases
ATGGCACAAGTTATTACGATTACTTCAGGTAAAGGCGGTGTGGGCAAATCTACCGCAACAGCAAATCTTGCTGTGGGTTT
AGCTATGGAGCTAGAATCTAGCGGGAAGAAAGTTGTAGCGATAGATTTTGATATTGGTTTGCGTAACCTTGATATGCTCC
TTGGGCTAGAAAATCGCATTGTATATGATGTCATTGATGTAATGGAAGGTAAATGCAATCTCTCACAAGCCCTCATCAAT
CATAAAAAAACAAAAAATCTTTACTTTCTTCCTGCTTCACAGACAAAGGATAAAACAATCCTTGATAAGGATAAAGTGGG
TAAACTCATTGCATCTTTGCGAGAAAATTTTGACTATATTCTTATAGATTCTCCAGCGGGGATTGAGAGTGGGTTTGAAC
ACGCAATATTATGGGCAGATAGAGCACTTATTGTTGTTACGTCGGAAGTGAGTTCGGTGCGAGATAGCGATAGGGTGATT
GGTATTATTGATGCAAAGAGCCACAAAGCACAACAAAATGAAGAAGTCCAAAAACATATCATCATCAATCGTATTAAGCC
AGAACTTGTAAAAAAGGGCGAAATGCTTTCAACCGATGATGTGCTAGGCATACTTGCTTTGCCTCTTATTGGGCTTATCC
CAGAGGATAGTAAAATTGTGAGTGCAACAAATTCGGGCGAACCTGTGATTTACACTCAAGCACCAAGTGCAAAAGCTTAT
GTGCGGATTGCTAAGAGAATCTTAGGACAAGAAGTGCCTTTTGTTTCTTTGGAAAGTGAGGGTGTTATGGGCACGCTTAA
AAGGATTTTTAAATGA

Upstream 100 bases:

>100_bases
GCTTGTTCAATGTTTAATAGCTTGCAACAAAAGCTCAAATAAGCGCATACTAAACTTTAGCATTATATAATGATATTTTA
ATTGATAAATGGGAGCAGAT

Downstream 100 bases:

>100_bases
GTCTTCTTGGTATGTTTGGACATAGCTCACAAAAAAGTGCTACTTTAGCAAGAGAGCGTCTTAAGTTGGTTTTATCGCAC
GAGAGGACAGCGAATATACC

Product: cell division inhibitor MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAQVITITSGKGGVGKSTATANLAVGLAMELESSGKKVVAIDFDIGLRNLDMLLGLENRIVYDVIDVMEGKCNLSQALIN
HKKTKNLYFLPASQTKDKTILDKDKVGKLIASLRENFDYILIDSPAGIESGFEHAILWADRALIVVTSEVSSVRDSDRVI
GIIDAKSHKAQQNEEVQKHIIINRIKPELVKKGEMLSTDDVLGILALPLIGLIPEDSKIVSATNSGEPVIYTQAPSAKAY
VRIAKRILGQEVPFVSLESEGVMGTLKRIFK

Sequences:

>Translated_271_residues
MAQVITITSGKGGVGKSTATANLAVGLAMELESSGKKVVAIDFDIGLRNLDMLLGLENRIVYDVIDVMEGKCNLSQALIN
HKKTKNLYFLPASQTKDKTILDKDKVGKLIASLRENFDYILIDSPAGIESGFEHAILWADRALIVVTSEVSSVRDSDRVI
GIIDAKSHKAQQNEEVQKHIIINRIKPELVKKGEMLSTDDVLGILALPLIGLIPEDSKIVSATNSGEPVIYTQAPSAKAY
VRIAKRILGQEVPFVSLESEGVMGTLKRIFK
>Mature_270_residues
AQVITITSGKGGVGKSTATANLAVGLAMELESSGKKVVAIDFDIGLRNLDMLLGLENRIVYDVIDVMEGKCNLSQALINH
KKTKNLYFLPASQTKDKTILDKDKVGKLIASLRENFDYILIDSPAGIESGFEHAILWADRALIVVTSEVSSVRDSDRVIG
IIDAKSHKAQQNEEVQKHIIINRIKPELVKKGEMLSTDDVLGILALPLIGLIPEDSKIVSATNSGEPVIYTQAPSAKAYV
RIAKRILGQEVPFVSLESEGVMGTLKRIFK

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=273, Percent_Identity=50.5494505494505, Blast_Score=272, Evalue=1e-74,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29481; Mature: 29350

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVITITSGKGGVGKSTATANLAVGLAMELESSGKKVVAIDFDIGLRNLDMLLGLENRI
CCEEEEEECCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCHH
VYDVIDVMEGKCNLSQALINHKKTKNLYFLPASQTKDKTILDKDKVGKLIASLRENFDYI
HHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LIDSPAGIESGFEHAILWADRALIVVTSEVSSVRDSDRVIGIIDAKSHKAQQNEEVQKHI
EEECCCCCCCCCCEEEEEECCEEEEEECCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
IINRIKPELVKKGEMLSTDDVLGILALPLIGLIPEDSKIVSATNSGEPVIYTQAPSAKAY
HHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCHHHH
VRIAKRILGQEVPFVSLESEGVMGTLKRIFK
HHHHHHHHCCCCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
AQVITITSGKGGVGKSTATANLAVGLAMELESSGKKVVAIDFDIGLRNLDMLLGLENRI
CEEEEEECCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCHH
VYDVIDVMEGKCNLSQALINHKKTKNLYFLPASQTKDKTILDKDKVGKLIASLRENFDYI
HHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LIDSPAGIESGFEHAILWADRALIVVTSEVSSVRDSDRVIGIIDAKSHKAQQNEEVQKHI
EEECCCCCCCCCCEEEEEECCEEEEEECCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
IINRIKPELVKKGEMLSTDDVLGILALPLIGLIPEDSKIVSATNSGEPVIYTQAPSAKAY
HHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCHHHH
VRIAKRILGQEVPFVSLESEGVMGTLKRIFK
HHHHHHHHCCCCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]