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The map label for this gene is fliM

Identifier: 32266646

GI number: 32266646

Start: 1112648

End: 1113709

Strand: Direct

Name: fliM

Synonym: HH1147

Alternate gene names: 32266646

Gene position: 1112648-1113709 (Clockwise)

Preceding gene: 161546627

Following gene: 32266647

Centisome position: 61.84

GC content: 37.38

Gene sequence:

>1062_bases
ATGGCTGATATATTAAGTCAAGAAGAAATTGACGCCCTTTTAGAAGTTGTTGATGATGATGGCGATACTGCTGATATAGA
GAAACAGGATTTAACACATCAAAAACAAGTTACGCTTTATGATTTTAAACGTCCTAATCGTGTGAGTAAAGAGCAGTTAC
GTGCTTTTAAATCAATTCACGATAAAATGGCGCGTAGTCTTTCATCTCAAGTTTCTGCGGTTATGCGAAGTATTGTGGAG
ATTCAACTACATAGTGTGGATCAAATGACTTATGGTGAGTTTTTGATGAGTTTGCCCTCTCCTACGAGCTTTAATATCTT
TTCAATGAAGCCACTAGAGGGCGCTGCAATTTTAGAGATTAATCCAAGTATTGCCTTTCCAATGATTGATAGACTTTTGG
GAGGGAAAGGCGACCCTTATGAAAGCTCACGTGAGTTTAGTGATATTGAACTCAATCTCCTTGATACACTTTTGCGTCAA
GTAATGCAAAATCTCAAAGAAGCGTGGAGCTCTATCACAGAGATTTTTCCAAGTGTTGATGCTAAGGAATCTTCTCCTAA
TGTTGTGCAAATCGTCGCACAAAATGAAATTGTTATTATGGTAGTTATGGAGATTATTATTGGGCATAGTAGCGGTATGA
TGAGTATTTGTTATCCTGTTATTTCGCTTGAAACAGTGCTTTCACGTTTGGCAAGTCGGGATTTAATGCTAAGTGATACA
AGTTCCAAGAAAAGTCGTAATAAGGAACTCCAAGCCCTTATCGCAGGGGCAAATGTAATAGTATCGGGCTATCTTGGTGA
TGCTACACTCACGCTTAAAGAAATTTTGGATTTGCAAGTAGGTGATATTGTGCGACTTGATACTATTGCTGATGATACTA
TAATCGTTTCTGTTGATGGGCGTGAAAAATATATTGCAAGTATAGGATTGCAACGTTATAGAAAGACATTGCAGATTAAA
GAAGTCATTAAAACAGAAAAAGACCAAGTAAAAGAAGTGCTTGAAATGCTTGAAGGGCAACGTAAAAATAGAATTGCAAA
TATTGCAGAGGAGGGAGAATGA

Upstream 100 bases:

>100_bases
CTTAAGGAAATCAGCGAGATTTTAGATATTACAGAATCTCGTATTTCGCAGATTACTAAGGAAATTGTCAAAAAAATTAG
ACAAGCATTAGGAGATATTA

Downstream 100 bases:

>100_bases
AAGCATTTGTTGATTTAGTTGTTACAGAGACAACAACGACCATTGAAGGCTTAATGGGGAAAACTCCAAGTGTAAGCCAT
ACGCAAGATGATAGTGTTAG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MADILSQEEIDALLEVVDDDGDTADIEKQDLTHQKQVTLYDFKRPNRVSKEQLRAFKSIHDKMARSLSSQVSAVMRSIVE
IQLHSVDQMTYGEFLMSLPSPTSFNIFSMKPLEGAAILEINPSIAFPMIDRLLGGKGDPYESSREFSDIELNLLDTLLRQ
VMQNLKEAWSSITEIFPSVDAKESSPNVVQIVAQNEIVIMVVMEIIIGHSSGMMSICYPVISLETVLSRLASRDLMLSDT
SSKKSRNKELQALIAGANVIVSGYLGDATLTLKEILDLQVGDIVRLDTIADDTIIVSVDGREKYIASIGLQRYRKTLQIK
EVIKTEKDQVKEVLEMLEGQRKNRIANIAEEGE

Sequences:

>Translated_353_residues
MADILSQEEIDALLEVVDDDGDTADIEKQDLTHQKQVTLYDFKRPNRVSKEQLRAFKSIHDKMARSLSSQVSAVMRSIVE
IQLHSVDQMTYGEFLMSLPSPTSFNIFSMKPLEGAAILEINPSIAFPMIDRLLGGKGDPYESSREFSDIELNLLDTLLRQ
VMQNLKEAWSSITEIFPSVDAKESSPNVVQIVAQNEIVIMVVMEIIIGHSSGMMSICYPVISLETVLSRLASRDLMLSDT
SSKKSRNKELQALIAGANVIVSGYLGDATLTLKEILDLQVGDIVRLDTIADDTIIVSVDGREKYIASIGLQRYRKTLQIK
EVIKTEKDQVKEVLEMLEGQRKNRIANIAEEGE
>Mature_352_residues
ADILSQEEIDALLEVVDDDGDTADIEKQDLTHQKQVTLYDFKRPNRVSKEQLRAFKSIHDKMARSLSSQVSAVMRSIVEI
QLHSVDQMTYGEFLMSLPSPTSFNIFSMKPLEGAAILEINPSIAFPMIDRLLGGKGDPYESSREFSDIELNLLDTLLRQV
MQNLKEAWSSITEIFPSVDAKESSPNVVQIVAQNEIVIMVVMEIIIGHSSGMMSICYPVISLETVLSRLASRDLMLSDTS
SKKSRNKELQALIAGANVIVSGYLGDATLTLKEILDLQVGDIVRLDTIADDTIIVSVDGREKYIASIGLQRYRKTLQIKE
VIKTEKDQVKEVLEMLEGQRKNRIANIAEEGE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=328, Percent_Identity=27.4390243902439, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39423; Mature: 39292

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADILSQEEIDALLEVVDDDGDTADIEKQDLTHQKQVTLYDFKRPNRVSKEQLRAFKSIH
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHCEEEEECCCCCCCCHHHHHHHHHHH
DKMARSLSSQVSAVMRSIVEIQLHSVDQMTYGEFLMSLPSPTSFNIFSMKPLEGAAILEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEE
NPSIAFPMIDRLLGGKGDPYESSREFSDIELNLLDTLLRQVMQNLKEAWSSITEIFPSVD
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AKESSPNVVQIVAQNEIVIMVVMEIIIGHSSGMMSICYPVISLETVLSRLASRDLMLSDT
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECC
SSKKSRNKELQALIAGANVIVSGYLGDATLTLKEILDLQVGDIVRLDTIADDTIIVSVDG
CCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECC
REKYIASIGLQRYRKTLQIKEVIKTEKDQVKEVLEMLEGQRKNRIANIAEEGE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADILSQEEIDALLEVVDDDGDTADIEKQDLTHQKQVTLYDFKRPNRVSKEQLRAFKSIH
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHCEEEEECCCCCCCCHHHHHHHHHHH
DKMARSLSSQVSAVMRSIVEIQLHSVDQMTYGEFLMSLPSPTSFNIFSMKPLEGAAILEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEE
NPSIAFPMIDRLLGGKGDPYESSREFSDIELNLLDTLLRQVMQNLKEAWSSITEIFPSVD
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AKESSPNVVQIVAQNEIVIMVVMEIIIGHSSGMMSICYPVISLETVLSRLASRDLMLSDT
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECC
SSKKSRNKELQALIAGANVIVSGYLGDATLTLKEILDLQVGDIVRLDTIADDTIIVSVDG
CCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECC
REKYIASIGLQRYRKTLQIKEVIKTEKDQVKEVLEMLEGQRKNRIANIAEEGE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]