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The map label for this gene is fliA

Identifier: 161546627

GI number: 161546627

Start: 1111948

End: 1112655

Strand: Direct

Name: fliA

Synonym: HH1146

Alternate gene names: 161546627

Gene position: 1111948-1112655 (Clockwise)

Preceding gene: 32266644

Following gene: 32266646

Centisome position: 61.8

GC content: 36.86

Gene sequence:

>708_bases
ATGAAAAGCATAAAAAATAACAACGGATATAATCAGACGCTCAAATATGCACAAGATGAGCTTGCTTTGCAATACCTTCC
GGCAGTTAAGGCGATGGCATTTCGCCTTAAGGAGCGTTTGCCTAGCTCTATTGAAATGGCAGATCTTGTATCTATTGGCA
CAGAAGAGCTTATTAAACTTGCTCGCCGTTATGATAGTAGCCTTAATGATTCTTTTTGGGGCTTTGCTAAAACGCGAGTG
AATGGAGCAATGCTTGATTATTTGCGAAGCCTTGATGTCCTTTCTCGTGCTAATCGTAAGCTTGTAAAGAGTATTGATTA
TGAAGTATCAAAATATTTTAATGAACACCAAGAAGAGCCAAGCGATGAATATCTTGCGAAAGTCCTTAAAGAAGATATAG
CAAAGATAAAAGAGGCGCGTATGGCTTCGGATATATACGCACTTGTGCCTATTGATGAACAATATAACGCTATTTGTGCG
GAAAATATACTTGAAGATGTGCAAAAAGATGAGCTTATGGAGATTATCTTTAATGTCCTTAAAAAGCTTGGTAAGCGTGA
GCAGATGATTATTCAGCTGTATTATTTTGAGGAGCTTAGTCTTAAGGAAATCAGCGAGATTTTAGATATTACAGAATCTC
GTATTTCGCAGATTACTAAGGAAATTGTCAAAAAAATTAGACAAGCATTAGGAGATATTAATGGCTGA

Upstream 100 bases:

>100_bases
AGATTCGGAATTTTATTAGAAGTATAGAATCTGATGATGAAGAAATGGCAGCACAGGCAGCACAACAAAATACGCAGAAA
GAGGGTTAAGAGGTATTTTA

Downstream 100 bases:

>100_bases
TATATTAAGTCAAGAAGAAATTGACGCCCTTTTAGAAGTTGTTGATGATGATGGCGATACTGCTGATATAGAGAAACAGG
ATTTAACACATCAAAAACAA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKSIKNNNGYNQTLKYAQDELALQYLPAVKAMAFRLKERLPSSIEMADLVSIGTEELIKLARRYDSSLNDSFWGFAKTRV
NGAMLDYLRSLDVLSRANRKLVKSIDYEVSKYFNEHQEEPSDEYLAKVLKEDIAKIKEARMASDIYALVPIDEQYNAICA
ENILEDVQKDELMEIIFNVLKKLGKREQMIIQLYYFEELSLKEISEILDITESRISQITKEIVKKIRQALGDING

Sequences:

>Translated_235_residues
MKSIKNNNGYNQTLKYAQDELALQYLPAVKAMAFRLKERLPSSIEMADLVSIGTEELIKLARRYDSSLNDSFWGFAKTRV
NGAMLDYLRSLDVLSRANRKLVKSIDYEVSKYFNEHQEEPSDEYLAKVLKEDIAKIKEARMASDIYALVPIDEQYNAICA
ENILEDVQKDELMEIIFNVLKKLGKREQMIIQLYYFEELSLKEISEILDITESRISQITKEIVKKIRQALGDING
>Mature_235_residues
MKSIKNNNGYNQTLKYAQDELALQYLPAVKAMAFRLKERLPSSIEMADLVSIGTEELIKLARRYDSSLNDSFWGFAKTRV
NGAMLDYLRSLDVLSRANRKLVKSIDYEVSKYFNEHQEEPSDEYLAKVLKEDIAKIKEARMASDIYALVPIDEQYNAICA
ENILEDVQKDELMEIIFNVLKKLGKREQMIIQLYYFEELSLKEISEILDITESRISQITKEIVKKIRQALGDING

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=221, Percent_Identity=30.7692307692308, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27176; Mature: 27176

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIKNNNGYNQTLKYAQDELALQYLPAVKAMAFRLKERLPSSIEMADLVSIGTEELIKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHH
ARRYDSSLNDSFWGFAKTRVNGAMLDYLRSLDVLSRANRKLVKSIDYEVSKYFNEHQEEP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDEYLAKVLKEDIAKIKEARMASDIYALVPIDEQYNAICAENILEDVQKDELMEIIFNVL
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLGKREQMIIQLYYFEELSLKEISEILDITESRISQITKEIVKKIRQALGDING
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSIKNNNGYNQTLKYAQDELALQYLPAVKAMAFRLKERLPSSIEMADLVSIGTEELIKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHH
ARRYDSSLNDSFWGFAKTRVNGAMLDYLRSLDVLSRANRKLVKSIDYEVSKYFNEHQEEP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDEYLAKVLKEDIAKIKEARMASDIYALVPIDEQYNAICAENILEDVQKDELMEIIFNVL
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLGKREQMIIQLYYFEELSLKEISEILDITESRISQITKEIVKKIRQALGDING
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]