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The map label for this gene is flhF

Identifier: 32266642

GI number: 32266642

Start: 1109219

End: 1110670

Strand: Direct

Name: flhF

Synonym: HH1143

Alternate gene names: 32266642

Gene position: 1109219-1110670 (Clockwise)

Preceding gene: 32266641

Following gene: 32266643

Centisome position: 61.65

GC content: 36.29

Gene sequence:

>1452_bases
ATGGCAAAAAAACTTCACACCTTTATGGGAGATACCCCAGCCCAAGCCCTTAAAAAGGCACAAGATACCTTTGGCAACGA
GATTTTACTCGTAGAAAATAAAGAGATTCGGAAAAAATCTCTTACACAATCGGGTTTATATGAGATAGTCATTGCAGTTG
ATGATGAAGGCACACCATCAACACCTACAGAATCTACAGATACAAAAGATACTCAAGAGAAATTAGCACCCAATAGTGTG
CAAAAAAGACTTGATGAGATTGCAGAGAAAAATATGGCAAAGAAGCGTCAGGAATCAAATAAGCCTAAAGTTTATGATGA
AGTAACGCTTCAACTTTCTGATGCGGTAAAACAAATATCACAAATTGCCAATGTGCCGAGCAATATGCCTGTAAATCCTA
AGATTCAACCCAAGCGTCCTTATCCTACGGCTAAAGCAACCTTTAACAAAGCAAACACAACGCATATTTCAAGTATAGAA
GATAAAATTGAGGATTTGCAACAAACTCGTTTAGAGCAAAGTATCAATCAAAAAATAGAACTTAAGCATATTAAAAATGA
GCTTGATGAGATTAACGATAAGATGAAAATTATCCAAAATATGCTGTGGGAGGAAAAAAGCCCCAAAAATGAAGGCATTA
ATATTCCTCAAGAGTTTGCAGAAATTTATAGAATCGCAAAAAGTAGCGGTATGAACAAGGAGCATTTGGATACAATTATG
CAATTATCATTAGAACTTATGCCGCTTAAGATGCGCTCAAATTCCACAACAATTAAACGTTATTTTCGCGAAGTATTGAG
AAAAATGATTTATTGTCGTCCTGAAAACCTCCAAAGCAATGCAAAAAAAATCATAATGCTTGTAGGACCTACAGGTGTGG
GAAAAACAACCACTCTAGCCAAACTTGCCGCGCGTTATTCTTTAATGCTTAATCAACGTTATCGTGTGGGTGTAGTTACG
CTTGATACTTATCGCTTAGCAGCGGTTGATCAGCTAATGGCTTATGCGCGTATGATGAAGCTAAGTATTGACACGGTGGT
AGAGCCAGAGGAATTTGGCAAGGCAATAGATTCCCTTAAGCATTGTGATTATATTCTCATTGATACAGCGGGGCATTCTC
AACACGACCGCAGTAAGCTTCAAGCGCTTAAATCTTACCTTAATAATGATTACAAAATTGATGTGAGTCTTGTGCTTGCG
ATTAATGCAAAATATGAAGATTTAAGAGATACTTATAATGCTTTTAGTGAAATGAATATTGATACATTAATTTTTAGCAA
GCTTGATGAAAGTCGCAACTTTGGTAATATGTTTTCACTTGTATATGAGACAAAAAAACCCATTAGTTATCTCTCAATCG
GGCAAGGTGTGCCAAATGATTTGATTTTAGCAGGCAATGATTATTTGGTAGATTGTTTGCTTGATGGATTTAAACGCCCA
GAATCTAAATAG

Upstream 100 bases:

>100_bases
TATATGTGAAAGTTTGTATCTTTGTGCAAGCAGTTAAACTTTACTAAAACATCAACAAGCTACAATAAGAATTTCTATTT
TGAGCTTATAGGCGGATACG

Downstream 100 bases:

>100_bases
CGGAAGTGCTAAATGATACAAAATCAAGCCCACGAGCTTCAAGCGCTCGTTCAAAGTCGGGATAAACGCAATTTTAGCAC
AACAAAATTTATTGCTATTA

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAKKLHTFMGDTPAQALKKAQDTFGNEILLVENKEIRKKSLTQSGLYEIVIAVDDEGTPSTPTESTDTKDTQEKLAPNSV
QKRLDEIAEKNMAKKRQESNKPKVYDEVTLQLSDAVKQISQIANVPSNMPVNPKIQPKRPYPTAKATFNKANTTHISSIE
DKIEDLQQTRLEQSINQKIELKHIKNELDEINDKMKIIQNMLWEEKSPKNEGINIPQEFAEIYRIAKSSGMNKEHLDTIM
QLSLELMPLKMRSNSTTIKRYFREVLRKMIYCRPENLQSNAKKIIMLVGPTGVGKTTTLAKLAARYSLMLNQRYRVGVVT
LDTYRLAAVDQLMAYARMMKLSIDTVVEPEEFGKAIDSLKHCDYILIDTAGHSQHDRSKLQALKSYLNNDYKIDVSLVLA
INAKYEDLRDTYNAFSEMNIDTLIFSKLDESRNFGNMFSLVYETKKPISYLSIGQGVPNDLILAGNDYLVDCLLDGFKRP
ESK

Sequences:

>Translated_483_residues
MAKKLHTFMGDTPAQALKKAQDTFGNEILLVENKEIRKKSLTQSGLYEIVIAVDDEGTPSTPTESTDTKDTQEKLAPNSV
QKRLDEIAEKNMAKKRQESNKPKVYDEVTLQLSDAVKQISQIANVPSNMPVNPKIQPKRPYPTAKATFNKANTTHISSIE
DKIEDLQQTRLEQSINQKIELKHIKNELDEINDKMKIIQNMLWEEKSPKNEGINIPQEFAEIYRIAKSSGMNKEHLDTIM
QLSLELMPLKMRSNSTTIKRYFREVLRKMIYCRPENLQSNAKKIIMLVGPTGVGKTTTLAKLAARYSLMLNQRYRVGVVT
LDTYRLAAVDQLMAYARMMKLSIDTVVEPEEFGKAIDSLKHCDYILIDTAGHSQHDRSKLQALKSYLNNDYKIDVSLVLA
INAKYEDLRDTYNAFSEMNIDTLIFSKLDESRNFGNMFSLVYETKKPISYLSIGQGVPNDLILAGNDYLVDCLLDGFKRP
ESK
>Mature_482_residues
AKKLHTFMGDTPAQALKKAQDTFGNEILLVENKEIRKKSLTQSGLYEIVIAVDDEGTPSTPTESTDTKDTQEKLAPNSVQ
KRLDEIAEKNMAKKRQESNKPKVYDEVTLQLSDAVKQISQIANVPSNMPVNPKIQPKRPYPTAKATFNKANTTHISSIED
KIEDLQQTRLEQSINQKIELKHIKNELDEINDKMKIIQNMLWEEKSPKNEGINIPQEFAEIYRIAKSSGMNKEHLDTIMQ
LSLELMPLKMRSNSTTIKRYFREVLRKMIYCRPENLQSNAKKIIMLVGPTGVGKTTTLAKLAARYSLMLNQRYRVGVVTL
DTYRLAAVDQLMAYARMMKLSIDTVVEPEEFGKAIDSLKHCDYILIDTAGHSQHDRSKLQALKSYLNNDYKIDVSLVLAI
NAKYEDLRDTYNAFSEMNIDTLIFSKLDESRNFGNMFSLVYETKKPISYLSIGQGVPNDLILAGNDYLVDCLLDGFKRPE
SK

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=228, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788963, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325345, Length=226, Percent_Identity=25.6637168141593, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 54974; Mature: 54843

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLHTFMGDTPAQALKKAQDTFGNEILLVENKEIRKKSLTQSGLYEIVIAVDDEGTPS
CCCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCC
TPTESTDTKDTQEKLAPNSVQKRLDEIAEKNMAKKRQESNKPKVYDEVTLQLSDAVKQIS
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QIANVPSNMPVNPKIQPKRPYPTAKATFNKANTTHISSIEDKIEDLQQTRLEQSINQKIE
HHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHIKNELDEINDKMKIIQNMLWEEKSPKNEGINIPQEFAEIYRIAKSSGMNKEHLDTIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QLSLELMPLKMRSNSTTIKRYFREVLRKMIYCRPENLQSNAKKIIMLVGPTGVGKTTTLA
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
KLAARYSLMLNQRYRVGVVTLDTYRLAAVDQLMAYARMMKLSIDTVVEPEEFGKAIDSLK
HHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HCDYILIDTAGHSQHDRSKLQALKSYLNNDYKIDVSLVLAINAKYEDLRDTYNAFSEMNI
HCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCH
DTLIFSKLDESRNFGNMFSLVYETKKPISYLSIGQGVPNDLILAGNDYLVDCLLDGFKRP
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCC
ESK
CCC
>Mature Secondary Structure 
AKKLHTFMGDTPAQALKKAQDTFGNEILLVENKEIRKKSLTQSGLYEIVIAVDDEGTPS
CCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCC
TPTESTDTKDTQEKLAPNSVQKRLDEIAEKNMAKKRQESNKPKVYDEVTLQLSDAVKQIS
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QIANVPSNMPVNPKIQPKRPYPTAKATFNKANTTHISSIEDKIEDLQQTRLEQSINQKIE
HHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHIKNELDEINDKMKIIQNMLWEEKSPKNEGINIPQEFAEIYRIAKSSGMNKEHLDTIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QLSLELMPLKMRSNSTTIKRYFREVLRKMIYCRPENLQSNAKKIIMLVGPTGVGKTTTLA
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
KLAARYSLMLNQRYRVGVVTLDTYRLAAVDQLMAYARMMKLSIDTVVEPEEFGKAIDSLK
HHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HCDYILIDTAGHSQHDRSKLQALKSYLNNDYKIDVSLVLAINAKYEDLRDTYNAFSEMNI
HCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCH
DTLIFSKLDESRNFGNMFSLVYETKKPISYLSIGQGVPNDLILAGNDYLVDCLLDGFKRP
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCC
ESK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]