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The map label for this gene is dnaN

Identifier: 32266625

GI number: 32266625

Start: 1094815

End: 1095876

Strand: Direct

Name: dnaN

Synonym: HH1126

Alternate gene names: 32266625

Gene position: 1094815-1095876 (Clockwise)

Preceding gene: 32266624

Following gene: 32266626

Centisome position: 60.85

GC content: 31.45

Gene sequence:

>1062_bases
ATGAAAATCTCACTTCCAAAAAGCTCATTAGAAACAATCCTCACAAACTGCCAAAATTTCCTTGACAAAAGAGACAGATC
TCAAATTACCTCTCATATCTACTTTGAAGCACAAGATGATAAACTTCTCCTTAAAGCCACAGATTATGAAATTTGGCTTG
AATCCTACATCAACATTCCTTGTCAAGCACAGGGAAATGCCACAGTCAATGGAAAACAGATTCTTGATATTATTAAACGC
CTCAAAGACGACAATGTTACTATCGAAACAAAACAAGATTCTCTTCACATATCTCAAGGGAAAGCACGCACAAAACTCCC
TATGTTTAATGCTGACGAATTCCCAAAATTCCCAGAGTATGATGCAAATGCACAAATTAATATAGAATCTGATAAATTTC
TCAATTCCATTAAAAAAATCAATCCTGCTGTAGATACAAATAATCCAAAATACGAGCTTAATGGTTCTCTACTTGACATT
AAAGATTATAGTTTTAATTTTGTAGCTACTGATACAAGACGACTTGCGCTCATTGAATATAAAAACCCATCTATTAATGC
TCTCTCACTTGTGATACCAAAAAAAGCAATTAATGAAATACAAAAACTCTTTGTAGATACACTTGAAATTTATCACAATC
CTACACATTTAATTCTTAAAAACAATGATTATACCTTTTATACAAAACTTATCAGTGGTAAATATCCTGATTATGAGAAA
ATCATTCCCAAAGAATTCAAACATAAAATCGTGCTTCCCAAAGACAAATTTGTTGATGCTTTACAATTCGTCAAATCACT
TGCAAATAACATCAAAATTACTCTTTCTCCAAATGAAGTGCTTTTTGAATCTCTTAATGAAGAAAGTGGTGAAGCTTCTA
CACAAATTGAAGTGCAAACAAACATTGAAAATCTTACGCTTGGCATTAACTCTAAATATATCTTAGATTTTCTTTCACAA
ATTGATGCAAATGAATTTACGCTTTGTATTAATGAACCAAATACACCATTTGTAGTAATGAGTGAGAATTTTTCAACTGT
TATTATGCCTGTGATTCTCTAA

Upstream 100 bases:

>100_bases
TTAAATATAAAAGTATAGGCGGATTAGTAGCACTTTTTCACTTTTTCACTTTCCCTACTACCCCTACTTTAAAATAATTC
TATTACAAAGGACATTGCAG

Downstream 100 bases:

>100_bases
AATTAAAAAACGGAGCTCATATCAATGGAAAAAAAAGAATATCAAGCAAGTAATATTAAAGTTTTAAAAGGCTTAGAAGC
TGTCCGAAAACGCCCTGGTA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKISLPKSSLETILTNCQNFLDKRDRSQITSHIYFEAQDDKLLLKATDYEIWLESYINIPCQAQGNATVNGKQILDIIKR
LKDDNVTIETKQDSLHISQGKARTKLPMFNADEFPKFPEYDANAQINIESDKFLNSIKKINPAVDTNNPKYELNGSLLDI
KDYSFNFVATDTRRLALIEYKNPSINALSLVIPKKAINEIQKLFVDTLEIYHNPTHLILKNNDYTFYTKLISGKYPDYEK
IIPKEFKHKIVLPKDKFVDALQFVKSLANNIKITLSPNEVLFESLNEESGEASTQIEVQTNIENLTLGINSKYILDFLSQ
IDANEFTLCINEPNTPFVVMSENFSTVIMPVIL

Sequences:

>Translated_353_residues
MKISLPKSSLETILTNCQNFLDKRDRSQITSHIYFEAQDDKLLLKATDYEIWLESYINIPCQAQGNATVNGKQILDIIKR
LKDDNVTIETKQDSLHISQGKARTKLPMFNADEFPKFPEYDANAQINIESDKFLNSIKKINPAVDTNNPKYELNGSLLDI
KDYSFNFVATDTRRLALIEYKNPSINALSLVIPKKAINEIQKLFVDTLEIYHNPTHLILKNNDYTFYTKLISGKYPDYEK
IIPKEFKHKIVLPKDKFVDALQFVKSLANNIKITLSPNEVLFESLNEESGEASTQIEVQTNIENLTLGINSKYILDFLSQ
IDANEFTLCINEPNTPFVVMSENFSTVIMPVIL
>Mature_353_residues
MKISLPKSSLETILTNCQNFLDKRDRSQITSHIYFEAQDDKLLLKATDYEIWLESYINIPCQAQGNATVNGKQILDIIKR
LKDDNVTIETKQDSLHISQGKARTKLPMFNADEFPKFPEYDANAQINIESDKFLNSIKKINPAVDTNNPKYELNGSLLDI
KDYSFNFVATDTRRLALIEYKNPSINALSLVIPKKAINEIQKLFVDTLEIYHNPTHLILKNNDYTFYTKLISGKYPDYEK
IIPKEFKHKIVLPKDKFVDALQFVKSLANNIKITLSPNEVLFESLNEESGEASTQIEVQTNIENLTLGINSKYILDFLSQ
IDANEFTLCINEPNTPFVVMSENFSTVIMPVIL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=21.3513513513514, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40400; Mature: 40400

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLPKSSLETILTNCQNFLDKRDRSQITSHIYFEAQDDKLLLKATDYEIWLESYINIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEECCHHHEEHHHCCCC
CQAQGNATVNGKQILDIIKRLKDDNVTIETKQDSLHISQGKARTKLPMFNADEFPKFPEY
CCCCCCCEECHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DANAQINIESDKFLNSIKKINPAVDTNNPKYELNGSLLDIKDYSFNFVATDTRRLALIEY
CCCEEEEECHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCEEEEEEEE
KNPSINALSLVIPKKAINEIQKLFVDTLEIYHNPTHLILKNNDYTFYTKLISGKYPDYEK
CCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCHHH
IIPKEFKHKIVLPKDKFVDALQFVKSLANNIKITLSPNEVLFESLNEESGEASTQIEVQT
CCCHHHCCCEECCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCCCCCEEEEEEE
NIENLTLGINSKYILDFLSQIDANEFTLCINEPNTPFVVMSENFSTVIMPVIL
CCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEC
>Mature Secondary Structure
MKISLPKSSLETILTNCQNFLDKRDRSQITSHIYFEAQDDKLLLKATDYEIWLESYINIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEECCHHHEEHHHCCCC
CQAQGNATVNGKQILDIIKRLKDDNVTIETKQDSLHISQGKARTKLPMFNADEFPKFPEY
CCCCCCCEECHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DANAQINIESDKFLNSIKKINPAVDTNNPKYELNGSLLDIKDYSFNFVATDTRRLALIEY
CCCEEEEECHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCEEEEEEEE
KNPSINALSLVIPKKAINEIQKLFVDTLEIYHNPTHLILKNNDYTFYTKLISGKYPDYEK
CCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCHHH
IIPKEFKHKIVLPKDKFVDALQFVKSLANNIKITLSPNEVLFESLNEESGEASTQIEVQT
CCCHHHCCCEECCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCCCCCEEEEEEE
NIENLTLGINSKYILDFLSQIDANEFTLCINEPNTPFVVMSENFSTVIMPVIL
CCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]