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The map label for this gene is nadE

Identifier: 32266507

GI number: 32266507

Start: 984364

End: 985188

Strand: Direct

Name: nadE

Synonym: HH1008

Alternate gene names: 32266507

Gene position: 984364-985188 (Clockwise)

Preceding gene: 32266501

Following gene: 32266508

Centisome position: 54.71

GC content: 35.76

Gene sequence:

>825_bases
ATGAAACATTTTGTGAATCCTTGCATACATTTTATTCAAAAGCAACTTCAAGAACGAGGTTTTAAGAAAGTAGTATTAGG
CTTAAGTGGTGGTATAGATTCTGCAGTAGTGGCTACTTTGGCTACTTTAGCCTTAGGGAGCGAAAATGTGCGAGCTCTTC
TTATGCCCTCTCTTAGTTCTAATGAGGAACACTTTAATGATGCTTTCAATCTTGCTCATAATCTTGAACTTGAAAGCAAA
ATTATCCAGCTTGCCCCATTTCAAGAGAATTTTGCAAAGCAAGAAGGTATGGATTTGAGCGGAAAATATATGGAAAAGCT
TGATATGAATCAAAAAATGCGTATGGGCAATTTTTGCGCAAGAATCCGTATGACTATGCTTTATGATTGCGCAAGTGCAG
ACAATGCCCTTGTATTAGGAACAAGCAATAAAAGTGAGATTCTATTAGGTTATGGCACAATATTTGGAGATTTAGCATAC
GCTATAAATCCTATTGGTGGGCTATATAAAACACAAATTTTTGCTTTCGCGCGAGCACTTAATGTGCCACAAGAGATTAT
TGCCAAAAAACCAAGTGCAGATTTATTCGCTAATCAAAGTGATGAAACCGATTTGGGATATAATTATGCGGACATTGATA
CATTCTTAGAAGCATTTGAGAAACTAGGAGGTGTTGAAGCTACACAAAATAAAGAACGAGAGCATATCAAAGAAAAACTT
AAAAATGCAGGATTTGAATGCAATATGATAGAATCTCTAAGCACTCGTGTATGGAATAACACCTTTAAGCGCACTAAGCC
AACCATATTAGAATATAAAGTTTAA

Upstream 100 bases:

>100_bases
AAAAAGGCTTAAATGTTGTTTATTCTTGTTAAAATTTTTGTTTATTTTTAGAGTATATTATAATTACGATACGAGATGAT
AAAAATTTTAAGGTATAGAA

Downstream 100 bases:

>100_bases
GGAGAATGTATGCGATGGATTGATGCATATTTTTATCAGCCAAATTTTACCCAAAAGCTTGTGAGTTTTGCGCTTTTGCC
TTTATCATTGCTTTATGGAA

Product: NH(3)-dependent NAD(+) synthetase NadE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKHFVNPCIHFIQKQLQERGFKKVVLGLSGGIDSAVVATLATLALGSENVRALLMPSLSSNEEHFNDAFNLAHNLELESK
IIQLAPFQENFAKQEGMDLSGKYMEKLDMNQKMRMGNFCARIRMTMLYDCASADNALVLGTSNKSEILLGYGTIFGDLAY
AINPIGGLYKTQIFAFARALNVPQEIIAKKPSADLFANQSDETDLGYNYADIDTFLEAFEKLGGVEATQNKEREHIKEKL
KNAGFECNMIESLSTRVWNNTFKRTKPTILEYKV

Sequences:

>Translated_274_residues
MKHFVNPCIHFIQKQLQERGFKKVVLGLSGGIDSAVVATLATLALGSENVRALLMPSLSSNEEHFNDAFNLAHNLELESK
IIQLAPFQENFAKQEGMDLSGKYMEKLDMNQKMRMGNFCARIRMTMLYDCASADNALVLGTSNKSEILLGYGTIFGDLAY
AINPIGGLYKTQIFAFARALNVPQEIIAKKPSADLFANQSDETDLGYNYADIDTFLEAFEKLGGVEATQNKEREHIKEKL
KNAGFECNMIESLSTRVWNNTFKRTKPTILEYKV
>Mature_274_residues
MKHFVNPCIHFIQKQLQERGFKKVVLGLSGGIDSAVVATLATLALGSENVRALLMPSLSSNEEHFNDAFNLAHNLELESK
IIQLAPFQENFAKQEGMDLSGKYMEKLDMNQKMRMGNFCARIRMTMLYDCASADNALVLGTSNKSEILLGYGTIFGDLAY
AINPIGGLYKTQIFAFARALNVPQEIIAKKPSADLFANQSDETDLGYNYADIDTFLEAFEKLGGVEATQNKEREHIKEKL
KNAGFECNMIESLSTRVWNNTFKRTKPTILEYKV

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=229, Percent_Identity=29.2576419213974, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_HELHP (Q7VHF9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860539.1
- ProteinModelPortal:   Q7VHF9
- SMR:   Q7VHF9
- GeneID:   1492697
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1008
- NMPDR:   fig|235279.1.peg.1008
- HOGENOM:   HBG351567
- OMA:   RDFVLGN
- BioCyc:   HHEP235279:HH_1008-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30670; Mature: 30670

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 29-29

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFVNPCIHFIQKQLQERGFKKVVLGLSGGIDSAVVATLATLALGSENVRALLMPSLSS
CCCHHHHHHHHHHHHHHHCCHHEEEHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
NEEHFNDAFNLAHNLELESKIIQLAPFQENFAKQEGMDLSGKYMEKLDMNQKMRMGNFCA
CHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
RIRMTMLYDCASADNALVLGTSNKSEILLGYGTIFGDLAYAINPIGGLYKTQIFAFARAL
HHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NVPQEIIAKKPSADLFANQSDETDLGYNYADIDTFLEAFEKLGGVEATQNKEREHIKEKL
CCCHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNAGFECNMIESLSTRVWNNTFKRTKPTILEYKV
HHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKHFVNPCIHFIQKQLQERGFKKVVLGLSGGIDSAVVATLATLALGSENVRALLMPSLSS
CCCHHHHHHHHHHHHHHHCCHHEEEHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
NEEHFNDAFNLAHNLELESKIIQLAPFQENFAKQEGMDLSGKYMEKLDMNQKMRMGNFCA
CHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
RIRMTMLYDCASADNALVLGTSNKSEILLGYGTIFGDLAYAINPIGGLYKTQIFAFARAL
HHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NVPQEIIAKKPSADLFANQSDETDLGYNYADIDTFLEAFEKLGGVEATQNKEREHIKEKL
CCCHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNAGFECNMIESLSTRVWNNTFKRTKPTILEYKV
HHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954