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The map label for this gene is lexA

Identifier: 32266427

GI number: 32266427

Start: 899641

End: 900399

Strand: Direct

Name: lexA

Synonym: HH0928

Alternate gene names: 32266427

Gene position: 899641-900399 (Clockwise)

Preceding gene: 32266426

Following gene: 32266428

Centisome position: 50.0

GC content: 36.36

Gene sequence:

>759_bases
TTGAAACTTATCTCGTGGAATGTTAATGGGTTGCGCGCCTGTATGAATAAAGGCTTTATGGATTTTTTTAATGAAGTCAA
TGCTGATATTTTTTGTATTCAAGAATCTAAAATGTGCAAAGAACAAGCTCAATTTCACTTTGAAAATTATAAAGAATATT
GGAATAGTGCAGAAAAAAAGGGGTATTCTGGCGTGGTAATCTTAAGTAAAACTCAACCTATCAATGTCGCCTACGATATG
GGAATAACATATCACGATAAAGAAGGACGCATTATTACAGCAGAATATCCACATTTTTATCTTGTCAATGTCTATACACC
CAATTCTAAACGTGAGCTAGAACGGCTAGATTATCGTATGAAATGGGAAGATGACTTCAGGGCATTTGTTAAAAACCTTG
AAAATCATAAATCCGTTATTATTTGTGGTGACCTCAATGTAGCTCACCAAGAAATTGACTTAAAAAACCCAAAAACAAAC
CGCCGCAACGCAGGATTCACAGATGAAGAGCGAAATAAAATGAGTGCGCTTTTAGACGCTGGATTTATTGATACTTTCCG
TTACTTTTATCCCACACTCACAGGGGCTTATAGTTGGTGGAGTTATATGGGCAAAGCACGCGAAAACAATACAGGGTGGC
GAATTGATTATTTTCTTTGCTCACAATCTCTCACTCCTTCTCTTAAAGACGCAAATATTTATCCGCATATTTTTGGTAGC
GACCATTGCCCTGTAGGCTTAGAACTTAAACAATTATAA

Upstream 100 bases:

>100_bases
GACCCTTTCATCTAGCTGGCCAAGGATATCACCCTTTCTCGGTGAAAACGGAAGTTCAAATCTTTCAAGGGTCGCCATTA
GTTTTGTTTAAGGATAGATT

Downstream 100 bases:

>100_bases
AAAGATGAGGGGAGGGATACATAATGAAAACGCACATTCTCAAGCTTCTTCAGCTTAAGCAGCTCTACAAACGCGTATCT
TGTGGTGAGCAATACACAAA

Product: exodeoxyribonuclease LexA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKLISWNVNGLRACMNKGFMDFFNEVNADIFCIQESKMCKEQAQFHFENYKEYWNSAEKKGYSGVVILSKTQPINVAYDM
GITYHDKEGRIITAEYPHFYLVNVYTPNSKRELERLDYRMKWEDDFRAFVKNLENHKSVIICGDLNVAHQEIDLKNPKTN
RRNAGFTDEERNKMSALLDAGFIDTFRYFYPTLTGAYSWWSYMGKARENNTGWRIDYFLCSQSLTPSLKDANIYPHIFGS
DHCPVGLELKQL

Sequences:

>Translated_252_residues
MKLISWNVNGLRACMNKGFMDFFNEVNADIFCIQESKMCKEQAQFHFENYKEYWNSAEKKGYSGVVILSKTQPINVAYDM
GITYHDKEGRIITAEYPHFYLVNVYTPNSKRELERLDYRMKWEDDFRAFVKNLENHKSVIICGDLNVAHQEIDLKNPKTN
RRNAGFTDEERNKMSALLDAGFIDTFRYFYPTLTGAYSWWSYMGKARENNTGWRIDYFLCSQSLTPSLKDANIYPHIFGS
DHCPVGLELKQL
>Mature_252_residues
MKLISWNVNGLRACMNKGFMDFFNEVNADIFCIQESKMCKEQAQFHFENYKEYWNSAEKKGYSGVVILSKTQPINVAYDM
GITYHDKEGRIITAEYPHFYLVNVYTPNSKRELERLDYRMKWEDDFRAFVKNLENHKSVIICGDLNVAHQEIDLKNPKTN
RRNAGFTDEERNKMSALLDAGFIDTFRYFYPTLTGAYSWWSYMGKARENNTGWRIDYFLCSQSLTPSLKDANIYPHIFGS
DHCPVGLELKQL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=47.0588235294118, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI18375507, Length=315, Percent_Identity=28.2539682539683, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=29.0441176470588, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=41.9607843137255, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=37.9310344827586, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=48.8, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=48.8, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29556; Mature: 29556

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRACMNKGFMDFFNEVNADIFCIQESKMCKEQAQFHFENYKEYWNSAEKK
CEEEEECCHHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
GYSGVVILSKTQPINVAYDMGITYHDKEGRIITAEYPHFYLVNVYTPNSKRELERLDYRM
CCCEEEEEECCCCCEEEEECCEEEECCCCCEEEEECCCEEEEEEECCCCHHHHHHCCCCC
KWEDDFRAFVKNLENHKSVIICGDLNVAHQEIDLKNPKTNRRNAGFTDEERNKMSALLDA
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTFRYFYPTLTGAYSWWSYMGKARENNTGWRIDYFLCSQSLTPSLKDANIYPHIFGS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEECCC
DHCPVGLELKQL
CCCCCCCEECCC
>Mature Secondary Structure
MKLISWNVNGLRACMNKGFMDFFNEVNADIFCIQESKMCKEQAQFHFENYKEYWNSAEKK
CEEEEECCHHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
GYSGVVILSKTQPINVAYDMGITYHDKEGRIITAEYPHFYLVNVYTPNSKRELERLDYRM
CCCEEEEEECCCCCEEEEECCEEEECCCCCEEEEECCCEEEEEEECCCCHHHHHHCCCCC
KWEDDFRAFVKNLENHKSVIICGDLNVAHQEIDLKNPKTNRRNAGFTDEERNKMSALLDA
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTFRYFYPTLTGAYSWWSYMGKARENNTGWRIDYFLCSQSLTPSLKDANIYPHIFGS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEECCC
DHCPVGLELKQL
CCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]