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The map label for this gene is cysK_2

Identifier: 32266348

GI number: 32266348

Start: 825490

End: 826392

Strand: Direct

Name: cysK_2

Synonym: HH0849

Alternate gene names: 32266348

Gene position: 825490-826392 (Clockwise)

Preceding gene: 32266344

Following gene: 32266353

Centisome position: 45.88

GC content: 39.42

Gene sequence:

>903_bases
ATGATAGCTCAAAATATTACCGAACTCATTGGCAACACTCCGATTTTACAACTTCAGCAATTTGCTCCAAATCTTTATGG
CAAATGCGAGTTTTTAAACCCAAGCCATTCTGTTAAAGACCGCCCCGCATATGCAATGATAGATGAGGCTCTAAAGACAG
GCAAAATCACAAAAGATACAACTATTGTAGAATGCACAAGCGGCAATATGGGCATATCTCTCGCTATGATTTGTGCTTCT
CTTGGATTAAAAATCATCATCACTATGCCAGAATCTATGAGCATAGAAAGACGCAAGATGATAGCTTTGTTTGGTGCGAA
TCTTGTGCTTACTCCTGCAAGTGAGGGTATGCAAGGTGCGCTCAATAAGGCTCAAGAAATTTTAGATTCCACACCAAACT
CATTTATGCCAAGTCAATTTGATAATCTCGCTAACAAAGAAATGCACAAACGCACTACTGCACTAGAGATTTATAAAAGC
TTTGAGGGAAGTTTGGATTATTTTGTGGCAGGATTTGGCACAGGTGGGACAATTAGCGGTGTAGGCGAAGTGCTTAAAGA
GAAGATTCCATCTATTAAAATTATTGGTGTAGAGCCTGCTGCTTCACCACTGCTTAGCAAAGGACAAGCGGGACCACATA
AGATTCAAGGCATTGGCGCGAACTTTATCCCTAAAATCCTTAATCGCGATGTGATAGATTCTATTGAAGTTGTAGAAAAT
GAAGTCGCTATTAAAATGGCTCAAAATTTAGGCAAAATCGGCGTAATGGTTGGAATTTCTAGTGGCGCAAATGTCGCAGC
TGCACTTGAAATTGCCAAAAACAATCCAAATAAAAAAGTGCTTACTATGCTTAATGACACAGCAGAGCGATACCTTTCAA
CGGATTTATTTAATACGCTTTAA

Upstream 100 bases:

>100_bases
AGAAAATTTGCATTATTTTCCTTGATTTTTTTATAAAAAATTGCTAGATTCTAAGCTTTAAATACCAAATTCACGCTTAA
ATGCGCACAAAGGATTTTCA

Downstream 100 bases:

>100_bases
ACCTTGAATGCTACTAAAAGCGGTGGATAATGAGACTTTGGCAATGCTTCCTTGATACAAGCAAAGTAACAAGAGTTTGA
AAACCGCATTCTCACTCTCA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIAQNITELIGNTPILQLQQFAPNLYGKCEFLNPSHSVKDRPAYAMIDEALKTGKITKDTTIVECTSGNMGISLAMICAS
LGLKIIITMPESMSIERRKMIALFGANLVLTPASEGMQGALNKAQEILDSTPNSFMPSQFDNLANKEMHKRTTALEIYKS
FEGSLDYFVAGFGTGGTISGVGEVLKEKIPSIKIIGVEPAASPLLSKGQAGPHKIQGIGANFIPKILNRDVIDSIEVVEN
EVAIKMAQNLGKIGVMVGISSGANVAAALEIAKNNPNKKVLTMLNDTAERYLSTDLFNTL

Sequences:

>Translated_300_residues
MIAQNITELIGNTPILQLQQFAPNLYGKCEFLNPSHSVKDRPAYAMIDEALKTGKITKDTTIVECTSGNMGISLAMICAS
LGLKIIITMPESMSIERRKMIALFGANLVLTPASEGMQGALNKAQEILDSTPNSFMPSQFDNLANKEMHKRTTALEIYKS
FEGSLDYFVAGFGTGGTISGVGEVLKEKIPSIKIIGVEPAASPLLSKGQAGPHKIQGIGANFIPKILNRDVIDSIEVVEN
EVAIKMAQNLGKIGVMVGISSGANVAAALEIAKNNPNKKVLTMLNDTAERYLSTDLFNTL
>Mature_300_residues
MIAQNITELIGNTPILQLQQFAPNLYGKCEFLNPSHSVKDRPAYAMIDEALKTGKITKDTTIVECTSGNMGISLAMICAS
LGLKIIITMPESMSIERRKMIALFGANLVLTPASEGMQGALNKAQEILDSTPNSFMPSQFDNLANKEMHKRTTALEIYKS
FEGSLDYFVAGFGTGGTISGVGEVLKEKIPSIKIIGVEPAASPLLSKGQAGPHKIQGIGANFIPKILNRDVIDSIEVVEN
EVAIKMAQNLGKIGVMVGISSGANVAAALEIAKNNPNKKVLTMLNDTAERYLSTDLFNTL

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=308, Percent_Identity=35.7142857142857, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI295821200, Length=308, Percent_Identity=35.7142857142857, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4557415, Length=308, Percent_Identity=35.7142857142857, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1788754, Length=304, Percent_Identity=44.7368421052632, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI2367138, Length=297, Percent_Identity=36.026936026936, Blast_Score=187, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17535051, Length=301, Percent_Identity=44.8504983388704, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17562970, Length=308, Percent_Identity=42.5324675324675, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=43.3333333333333, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=42.671009771987, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17561720, Length=308, Percent_Identity=41.8831168831169, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=41.3953488372093, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17534315, Length=315, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147552, Length=321, Percent_Identity=33.9563862928349, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=43.6974789915966, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539800, Length=346, Percent_Identity=25.4335260115607, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996324, Length=335, Percent_Identity=25.6716417910448, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=34.2857142857143, Blast_Score=178, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=32.6219512195122, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=37.012987012987, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=37.012987012987, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- InterPro:   IPR000634 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32174; Mature: 32174

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQNITELIGNTPILQLQQFAPNLYGKCEFLNPSHSVKDRPAYAMIDEALKTGKITKDT
CCCHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TIVECTSGNMGISLAMICASLGLKIIITMPESMSIERRKMIALFGANLVLTPASEGMQGA
EEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHEECCCEEEECCCCCHHHH
LNKAQEILDSTPNSFMPSQFDNLANKEMHKRTTALEIYKSFEGSLDYFVAGFGTGGTISG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
VGEVLKEKIPSIKIIGVEPAASPLLSKGQAGPHKIQGIGANFIPKILNRDVIDSIEVVEN
HHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCEECCCCHHHHHHHCHHHHHHHHHHHH
EVAIKMAQNLGKIGVMVGISSGANVAAALEIAKNNPNKKVLTMLNDTAERYLSTDLFNTL
HHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAQNITELIGNTPILQLQQFAPNLYGKCEFLNPSHSVKDRPAYAMIDEALKTGKITKDT
CCCHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TIVECTSGNMGISLAMICASLGLKIIITMPESMSIERRKMIALFGANLVLTPASEGMQGA
EEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHEECCCEEEECCCCCHHHH
LNKAQEILDSTPNSFMPSQFDNLANKEMHKRTTALEIYKSFEGSLDYFVAGFGTGGTISG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
VGEVLKEKIPSIKIIGVEPAASPLLSKGQAGPHKIQGIGANFIPKILNRDVIDSIEVVEN
HHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCEECCCCHHHHHHHCHHHHHHHHHHHH
EVAIKMAQNLGKIGVMVGISSGANVAAALEIAKNNPNKKVLTMLNDTAERYLSTDLFNTL
HHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9034314; 10688204 [H]