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The map label for this gene is trmB

Identifier: 32266284

GI number: 32266284

Start: 761747

End: 762937

Strand: Direct

Name: trmB

Synonym: HH0785

Alternate gene names: 32266284

Gene position: 761747-762937 (Clockwise)

Preceding gene: 32266283

Following gene: 32266285

Centisome position: 42.34

GC content: 34.17

Gene sequence:

>1191_bases
ATGCCTCATTTTCTTGCATCTCATATTACATTACCACCTATGCCTTTTACACAAGAAGGCTATACCTTTGTATATGAAGC
CAAACAATGCAACGATTCTTCACAATCACTTATTTTGGTGCGATATGAAGATAATGAGTTTTTTTTACGCAAAGCGTGGC
GAGGCAATAAAAATAATGCTATTCTCAAATGTGAAAAAAGCACAAAAACTCAACCTACAGGCATTATTAAGAATGCCCTT
AAGATTCTATGTGCGTATCAAAAAAATGTTATCTCTCATAACCTCAACAAAAATACGCCACGACAAAATCTCCAATCTCC
CTATTTTCAGTCTATGGATTTTTTTTTGGATTTTGCAAAGCCTTGCCTTATTGAAATAGGCTTTGGCTCTGGGCGCCACC
TTTTGCATCTTGCACAGAGCAACCCTCATTTTATGTGTATTGGTATAGAAATTCATACTCCCTCTATTGAGCAGATTCTT
AGGCAAGTGCAACTCTTGGGTTTAGAGAATCTTTATATCATTAATGGCGATGCGCGTATCCTCCTTGAAATTCTCTCCTC
TCATATTGCACAAGGTATTTATGTGCATTTTCCTGTGCCGTGGAATAAAAAACACCATAGGCGCATTTTTTCGCCCAAAT
TTTTTCAGGAATCTTTGCGCGTTTTAGATAATAATGGAGTGCTTCATTTGCGCAGTGATGATGAAATTTATTTCCAAGAT
GCCCTTTCCCTTGCCTTGCAACAAGAAAGCATAAGCCTTGAAATACACAAGAATCAACAAGAAGTAATTACAAGCAAATA
CGAAGCAAGATGGAAAAAACAACAAAAAAACATTTATGATTTAAAGATTTTTCATACTTATAAAAACACAAATAATGAAA
AAAATGTAAAAAATACCCAAAAAAACTTTTATTTTGATAAAATTCTAAGAAAAAATTTGGATAATTACAAAAATTTTCCA
CATAAGAAAATTGCTAATGATTGGTTTTTGCATATTGATAATCTGTATTGTGCGGGGGATATATATGTATTGGCATTATG
TTTTGGGGATTTTAACCAGCCTCAAAGCAAGTTTTTGCAAATTGAATTCTGCAATAATGTATCAGCTCGGTATATTGGTA
GTAACCCTATCCCTACACAAGCAGCCATCAAGGCGCATAAATATTTAATACAGATTCTAACACAGGAGTAA

Upstream 100 bases:

>100_bases
AAGGTATCAGTTATAAATATAGCGTAACAAGTATTGACAAAAATGGTATAGAATCTGCGCCCACACAAGAAATAGAACTC
TCCATAGAGGCTCAAAAATA

Downstream 100 bases:

>100_bases
AATTTGAGCACGATTATAGAAGCAAAAAATCTCAATCTAGGCTATAATGATGATCTTGTCATTAAAGATGCCACTTTTAA
TATTAACGCTAAAGAGTTTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPHFLASHITLPPMPFTQEGYTFVYEAKQCNDSSQSLILVRYEDNEFFLRKAWRGNKNNAILKCEKSTKTQPTGIIKNAL
KILCAYQKNVISHNLNKNTPRQNLQSPYFQSMDFFLDFAKPCLIEIGFGSGRHLLHLAQSNPHFMCIGIEIHTPSIEQIL
RQVQLLGLENLYIINGDARILLEILSSHIAQGIYVHFPVPWNKKHHRRIFSPKFFQESLRVLDNNGVLHLRSDDEIYFQD
ALSLALQQESISLEIHKNQQEVITSKYEARWKKQQKNIYDLKIFHTYKNTNNEKNVKNTQKNFYFDKILRKNLDNYKNFP
HKKIANDWFLHIDNLYCAGDIYVLALCFGDFNQPQSKFLQIEFCNNVSARYIGSNPIPTQAAIKAHKYLIQILTQE

Sequences:

>Translated_396_residues
MPHFLASHITLPPMPFTQEGYTFVYEAKQCNDSSQSLILVRYEDNEFFLRKAWRGNKNNAILKCEKSTKTQPTGIIKNAL
KILCAYQKNVISHNLNKNTPRQNLQSPYFQSMDFFLDFAKPCLIEIGFGSGRHLLHLAQSNPHFMCIGIEIHTPSIEQIL
RQVQLLGLENLYIINGDARILLEILSSHIAQGIYVHFPVPWNKKHHRRIFSPKFFQESLRVLDNNGVLHLRSDDEIYFQD
ALSLALQQESISLEIHKNQQEVITSKYEARWKKQQKNIYDLKIFHTYKNTNNEKNVKNTQKNFYFDKILRKNLDNYKNFP
HKKIANDWFLHIDNLYCAGDIYVLALCFGDFNQPQSKFLQIEFCNNVSARYIGSNPIPTQAAIKAHKYLIQILTQE
>Mature_395_residues
PHFLASHITLPPMPFTQEGYTFVYEAKQCNDSSQSLILVRYEDNEFFLRKAWRGNKNNAILKCEKSTKTQPTGIIKNALK
ILCAYQKNVISHNLNKNTPRQNLQSPYFQSMDFFLDFAKPCLIEIGFGSGRHLLHLAQSNPHFMCIGIEIHTPSIEQILR
QVQLLGLENLYIINGDARILLEILSSHIAQGIYVHFPVPWNKKHHRRIFSPKFFQESLRVLDNNGVLHLRSDDEIYFQDA
LSLALQQESISLEIHKNQQEVITSKYEARWKKQQKNIYDLKIFHTYKNTNNEKNVKNTQKNFYFDKILRKNLDNYKNFPH
KKIANDWFLHIDNLYCAGDIYVLALCFGDFNQPQSKFLQIEFCNNVSARYIGSNPIPTQAAIKAHKYLIQILTQE

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_HELHP (Q7VI24)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860316.1
- ProteinModelPortal:   Q7VI24
- SMR:   Q7VI24
- GeneID:   1492474
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0785
- NMPDR:   fig|235279.1.peg.785
- HOGENOM:   HBG321449
- ProtClustDB:   PRK14121
- BioCyc:   HHEP235279:HH_0785-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 46254; Mature: 46122

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: BINDING 125-125 BINDING 150-150 BINDING 177-177 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFLASHITLPPMPFTQEGYTFVYEAKQCNDSSQSLILVRYEDNEFFLRKAWRGNKNNA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCEEEEHHCCCCCCCE
ILKCEKSTKTQPTGIIKNALKILCAYQKNVISHNLNKNTPRQNLQSPYFQSMDFFLDFAK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCC
PCLIEIGFGSGRHLLHLAQSNPHFMCIGIEIHTPSIEQILRQVQLLGLENLYIINGDARI
CEEEEEECCCCCEEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHH
LLEILSSHIAQGIYVHFPVPWNKKHHRRIFSPKFFQESLRVLDNNGVLHLRSDDEIYFQD
HHHHHHHHHCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
ALSLALQQESISLEIHKNQQEVITSKYEARWKKQQKNIYDLKIFHTYKNTNNEKNVKNTQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHH
KNFYFDKILRKNLDNYKNFPHKKIANDWFLHIDNLYCAGDIYVLALCFGDFNQPQSKFLQ
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCEEECCHHEEEEEECCCCCCCHHHEEE
IEFCNNVSARYIGSNPIPTQAAIKAHKYLIQILTQE
EEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PHFLASHITLPPMPFTQEGYTFVYEAKQCNDSSQSLILVRYEDNEFFLRKAWRGNKNNA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCEEEEHHCCCCCCCE
ILKCEKSTKTQPTGIIKNALKILCAYQKNVISHNLNKNTPRQNLQSPYFQSMDFFLDFAK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCC
PCLIEIGFGSGRHLLHLAQSNPHFMCIGIEIHTPSIEQILRQVQLLGLENLYIINGDARI
CEEEEEECCCCCEEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHH
LLEILSSHIAQGIYVHFPVPWNKKHHRRIFSPKFFQESLRVLDNNGVLHLRSDDEIYFQD
HHHHHHHHHCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
ALSLALQQESISLEIHKNQQEVITSKYEARWKKQQKNIYDLKIFHTYKNTNNEKNVKNTQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHH
KNFYFDKILRKNLDNYKNFPHKKIANDWFLHIDNLYCAGDIYVLALCFGDFNQPQSKFLQ
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCEEECCHHEEEEEECCCCCCCHHHEEE
IEFCNNVSARYIGSNPIPTQAAIKAHKYLIQILTQE
EEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954