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The map label for this gene is rnhA

Identifier: 32266197

GI number: 32266197

Start: 678422

End: 678868

Strand: Direct

Name: rnhA

Synonym: HH0698

Alternate gene names: 32266197

Gene position: 678422-678868 (Clockwise)

Preceding gene: 32266196

Following gene: 32266198

Centisome position: 37.71

GC content: 36.02

Gene sequence:

>447_bases
TTGATAATGAAACAAGTTACTCTTTATTGTGATGGTAGTGCACTTGGAAATCCCGGTGCTGGAGGCTGGTGTGGCATACT
CTCTTTTGGTGATAAGCAAAAGATTCTTACAGGTGGCGAAACCTATACAACAAATAATCGTATGGAACTCCTTGCAGTGA
TAGAATCTCTCAAAGCTCTTAATCAACCTTGCATTGTGAATGTATATAGTGATTCTCGCTATGTATGCAATGGTATCAAC
TTATGGCTTAAATCGTGGATTTCTAAGCAATTTAAAAATGTCAAAAATCCTGATTTATGGCAACTATATTTACAAGTTTC
ATCACCTCATCAAGTGATTGCACATTGGGTAAAAGGACACGCGGGAGTAGCACAAAATGAACTATGTGATAAACTTGCAA
AAGAAAGTGCGCAATTTTATTTAAATAAAGGAATTTCTGATGAGTAA

Upstream 100 bases:

>100_bases
CGCTGCCATAGCTGCAAACAAGTATTTCCCTTTGAAAACGCACGTTGTTCACATTGTGGTGAGTTGCTTAATACCGATGT
AATCTATAAGATACAAAAGG

Downstream 100 bases:

>100_bases
ATCTATTTTGCCCAAATCTCCCCAGCTCCTTGAGAAAAAGTTAGGCTATGTTTTTCAAAATCAACAACTCCTCCTTGAAG
CCCTCACGCATAAAAGTTGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MIMKQVTLYCDGSALGNPGAGGWCGILSFGDKQKILTGGETYTTNNRMELLAVIESLKALNQPCIVNVYSDSRYVCNGIN
LWLKSWISKQFKNVKNPDLWQLYLQVSSPHQVIAHWVKGHAGVAQNELCDKLAKESAQFYLNKGISDE

Sequences:

>Translated_148_residues
MIMKQVTLYCDGSALGNPGAGGWCGILSFGDKQKILTGGETYTTNNRMELLAVIESLKALNQPCIVNVYSDSRYVCNGIN
LWLKSWISKQFKNVKNPDLWQLYLQVSSPHQVIAHWVKGHAGVAQNELCDKLAKESAQFYLNKGISDE
>Mature_148_residues
MIMKQVTLYCDGSALGNPGAGGWCGILSFGDKQKILTGGETYTTNNRMELLAVIESLKALNQPCIVNVYSDSRYVCNGIN
LWLKSWISKQFKNVKNPDLWQLYLQVSSPHQVIAHWVKGHAGVAQNELCDKLAKESAQFYLNKGISDE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=46.0992907801418, Blast_Score=130, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16468; Mature: 16468

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKQVTLYCDGSALGNPGAGGWCGILSFGDKQKILTGGETYTTNNRMELLAVIESLKAL
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEECCCEEECCCHHHHHHHHHHHHHC
NQPCIVNVYSDSRYVCNGINLWLKSWISKQFKNVKNPDLWQLYLQVSSPHQVIAHWVKGH
CCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCC
AGVAQNELCDKLAKESAQFYLNKGISDE
CCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIMKQVTLYCDGSALGNPGAGGWCGILSFGDKQKILTGGETYTTNNRMELLAVIESLKAL
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEECCCEEECCCHHHHHHHHHHHHHC
NQPCIVNVYSDSRYVCNGINLWLKSWISKQFKNVKNPDLWQLYLQVSSPHQVIAHWVKGH
CCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCC
AGVAQNELCDKLAKESAQFYLNKGISDE
CCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA