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The map label for this gene is trmA

Identifier: 32266192

GI number: 32266192

Start: 673245

End: 674459

Strand: Direct

Name: trmA

Synonym: HH0693

Alternate gene names: 32266192

Gene position: 673245-674459 (Clockwise)

Preceding gene: 32266191

Following gene: 32266195

Centisome position: 37.42

GC content: 35.88

Gene sequence:

>1215_bases
ATGAATTGTGAGCATTTTGGCATATGTGGAGGCTGCACAAATATACAAGATTATTCCACACAACTGCAGGCAAAACACAA
TCTTACTTTGCAAGAATTTCAATCTTTTCTTGATACAAAAAGCAAAAATTTTCAATATCCCACCAATCCCTTAGCTATAG
AAGTATTTGCTTCACCGCAAGAGGGGTTTAGAGCTCGTGCAGAGTTTAGATTCTCTCACGTTTTCCAGAATAAAAGTGGG
CTTGATTTTGCTATGAATGCATTTGGTTATAATCACCGTGTGCCTATTAAAAAATGTCCTATTTTGCTCCCTACTTTACA
GGACATTATGCCTCTTTTGCTGCACTATCTCAATACATACGACTTACTTAATCATAAACTTTATGCGTGCAATCTCCTCT
CCTCCTTACAAAATGAAATTATCATCACCCTCATTTATCACAAATCCCTAGATTCTCATTGGGAATCTCTTGCGTTAAAA
ATACAAAAAGAGCTTGAATATACTCTTAATACGAATATTCACATTATCGGACGCAGTAAAAATCACAAACATATCCTCTC
AAATGATATTATTTGTGAGCATCTCACACTTTTTGCCAACACTCCAAAAGAACGCACTTATACATTTTTCAAACAAGAAT
CCCGATTTTGCCAACCTAATCCATTTATCAACACTCAAATGCTTGAATTTATTGTCTCCGCACTCTCATCAATCTATACC
CCACAAACTCCTTGTGATATGCTTGAACTCTATTGTGGTAGCGGTAACTTCACCATTCCACTTGCCTCAATATTCCGCCA
TATTTTTGCTACGGAAGTGGTAAAGTCTGCAATTACACTTTTACAAAAAAATATGGCTAAAAATAATATAGAAAATATTA
TACCTGCGCGACTTAACGCATTTGAAAGTATCCAAGCTCTGCGTAAAGAGCGCGTATTTTTTCGCCTTAAAAATATTGAT
TTAGATGCTTTTGCGTTTGATTGTGTATTAATTGACCCACCTCGTAGCGGTGTAGGCGAAGAAGAAGTGTTGTATTTTTT
GCAAAACTTTAACACAATTATATATGTCTCGTGCAATCCCCATACGCTACTAAACGATTTGCGCATTCTTTCTCAAAGCC
ATTATGTAATGCGATTTGGGCTTTTTGACCAATTCCCACATACATATCATAGAGAGTGCATAGTAATTTTGCGCAAATCC
AATAAGATTCTATAA

Upstream 100 bases:

>100_bases
TGATTTCGTTGCCTTTTAAGATTCTTGTTTTTATACTTGTTGATGGTTGGAATCTCCTCATAGGTAACCTTGTGCAAGGC
TTTAAGTAGCTAGGTCTAGA

Downstream 100 bases:

>100_bases
AATTATTCCATCTTACGTAGCACTAAAATAAATGCAGGAAGTGCAAAAAGCGAAGCAAAAAGAGGATTAAAACTAACACC
CAAAAATGGTGCAACAACGA

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQEGFRARAEFRFSHVFQNKSG
LDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTYDLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALK
IQKELEYTLNTNIHIIGRSKNHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYT
PQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNAFESIQALRKERVFFRLKNID
LDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNPHTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKS
NKIL

Sequences:

>Translated_404_residues
MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQEGFRARAEFRFSHVFQNKSG
LDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTYDLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALK
IQKELEYTLNTNIHIIGRSKNHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYT
PQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNAFESIQALRKERVFFRLKNID
LDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNPHTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKS
NKIL
>Mature_404_residues
MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQEGFRARAEFRFSHVFQNKSG
LDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTYDLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALK
IQKELEYTLNTNIHIIGRSKNHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYT
PQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNAFESIQALRKERVFFRLKNID
LDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNPHTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKS
NKIL

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Escherichia coli, GI1790403, Length=375, Percent_Identity=36.2666666666667, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789148, Length=409, Percent_Identity=25.9168704156479, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787083, Length=186, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=191, Percent_Identity=25.6544502617801, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_HELHP (Q7U326)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860224.1
- ProteinModelPortal:   Q7U326
- SMR:   Q7U326
- GeneID:   1493747
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0693
- NMPDR:   fig|235279.1.peg.693
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- BioCyc:   HHEP235279:HH_0693-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR010280

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 46805; Mature: 46805

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 358-358 BINDING 218-218 BINDING 251-251 BINDING 256-256 BINDING 272-272 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQ
CCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCH
EGFRARAEFRFSHVFQNKSGLDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTY
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHCCEEHHHHHHHHHHHHHHHHHH
DLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALKIQKELEYTLNTNIHIIGRSK
HHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHEECCCEEEEECCC
NHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYT
CCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNA
CCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FESIQALRKERVFFRLKNIDLDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNP
HHHHHHHHHHHHHEEEECCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCC
HTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKSNKIL
HHHHHHHHHHHCCCCEEEECCHHHCCCHHCCEEEEEEECCCCCC
>Mature Secondary Structure
MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQ
CCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCH
EGFRARAEFRFSHVFQNKSGLDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTY
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHCCEEHHHHHHHHHHHHHHHHHH
DLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALKIQKELEYTLNTNIHIIGRSK
HHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHEECCCEEEEECCC
NHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYT
CCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNA
CCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FESIQALRKERVFFRLKNIDLDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNP
HHHHHHHHHHHHHEEEECCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCC
HTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKSNKIL
HHHHHHHHHHHCCCCEEEECCHHHCCCHHCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954