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The map label for this gene is radA [H]

Identifier: 32266090

GI number: 32266090

Start: 568538

End: 569884

Strand: Direct

Name: radA [H]

Synonym: HH0591

Alternate gene names: 32266090

Gene position: 568538-569884 (Clockwise)

Preceding gene: 32266089

Following gene: 32266091

Centisome position: 31.6

GC content: 39.2

Gene sequence:

>1347_bases
ATGGCAAAAAAAACTTCCATTTTTGAATGTCAATTTTGCGGGTGGCAAAGTGCAAAATGGCTTGGTAAATGCAGTAGCTG
TGGAAGTTGGGATTCGCTTATAGAACTTAAACCTAGTCAAATAAAAATCACAAAATCCCTCAAAAGCAATCCTCAAAACT
TGTCAAATGCTATCCCTATCACACAAGTAAGCTTTGAAAATATTGAGCGCTTTAGCTCCTTTGAAGAAGAATTGGATATT
GTATTAGGTGGAGGCATTGTCCCGGGCGGATTATACCTTATAGGGGGGAGTCCGGGAGTAGGAAAATCCACGCTTTTACT
GAAAATCGCTGGAGAAATTGCCAAACACATTCACAAAAAGGTTCTTTATGTAAGCGGTGAAGAGAGTGCGGGACAAATCA
AAATGCGTGCCTCTAGGCTTGGGGCGATAAGCGAAGATTTGTTTTTGCTTAATGAAATTGATTTAAATATCATTCAAAAT
GCACTTGATGAGCAGCAATATGCTCTATGTGTGATAGATTCTATCCAAACGATTTATTCTCCCGATATTACTTCTGCACC
TGGTTCTATCTCACAGGTGCGCGAAGTTACTTTTGCACTTATGAGACTTGCTAAGGAGCGCAATATAAGCATTTTTATCA
TCGGACATATTACCAAAGAAGGTTCAATTGCTGGACCAAGAATTTTAGAACATATGGTAGATTGTGTGCTGTATTTTGAA
GGAGATCCAAGCCGAGAGCTTAGAATGTTAAGGGGATTTAAAAATCGCTTTGGCACGACAAGCGAAATTGGTATTTTTGA
GATGAAAGAGGGAGGATTAGTAAGCGCTAAAAATGCTTCAAAACTCTTTTTTACGCAAAAAAGTGCTCAAGCAGGAAGTG
CTATAGCTGTGATACTTGAAGGAAGTCGTGCGCTTGTTATTGAGATTCAAGCACTTGTGAGTGAATCTTCTTATCCTAAA
CGCTCGTGCACAGGCTTTGATACTAATCGCCTCAATATGCTTCTTGCTCTCTTAGAACGCAAACTTGAACTTCCATTAGG
GCGCTATGATGTCTTTGTCAATGTTGCAGGCGGCATTAAAATCAATGAACCAAGCGCAGACCTTGCTGTAATTGCAAGCA
TCATCTCAAGCTTTCGTAATCGCCCTCTTAATGCAAAAACTGCTTTTATAGGCGAAGTTTCACTCACAGGCGATATACGA
GAAGTAAGCAATATTGATACACGTTTAAAAGAGCTTGAAAGCTATGGTTTTAACAAAGTTATACTTGCCAAAAAACCATC
TAACCCCTCATCTACACTTAAATGTTTTGAAACAAATGAGGTAAGCAGGATTTTAGAGTGGATGTGA

Upstream 100 bases:

>100_bases
TTTTATACATTGGTATAGGCGAAAGGGAAAATGACCTTATCCCTTTTGATTCTAAAGAATATGTAAGAATCTTGCTTGAT
AGTATTTATCAAGGACATTA

Downstream 100 bases:

>100_bases
AATTATCAAATTGTTGATAAATAAAAACTTTGATTTTTTAGTAAAAAATATTATAATTGTATCTTACAAGAATTTGATGA
TTTGGGGGTAATTTTTTTGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAKKTSIFECQFCGWQSAKWLGKCSSCGSWDSLIELKPSQIKITKSLKSNPQNLSNAIPITQVSFENIERFSSFEEELDI
VLGGGIVPGGLYLIGGSPGVGKSTLLLKIAGEIAKHIHKKVLYVSGEESAGQIKMRASRLGAISEDLFLLNEIDLNIIQN
ALDEQQYALCVIDSIQTIYSPDITSAPGSISQVREVTFALMRLAKERNISIFIIGHITKEGSIAGPRILEHMVDCVLYFE
GDPSRELRMLRGFKNRFGTTSEIGIFEMKEGGLVSAKNASKLFFTQKSAQAGSAIAVILEGSRALVIEIQALVSESSYPK
RSCTGFDTNRLNMLLALLERKLELPLGRYDVFVNVAGGIKINEPSADLAVIASIISSFRNRPLNAKTAFIGEVSLTGDIR
EVSNIDTRLKELESYGFNKVILAKKPSNPSSTLKCFETNEVSRILEWM

Sequences:

>Translated_448_residues
MAKKTSIFECQFCGWQSAKWLGKCSSCGSWDSLIELKPSQIKITKSLKSNPQNLSNAIPITQVSFENIERFSSFEEELDI
VLGGGIVPGGLYLIGGSPGVGKSTLLLKIAGEIAKHIHKKVLYVSGEESAGQIKMRASRLGAISEDLFLLNEIDLNIIQN
ALDEQQYALCVIDSIQTIYSPDITSAPGSISQVREVTFALMRLAKERNISIFIIGHITKEGSIAGPRILEHMVDCVLYFE
GDPSRELRMLRGFKNRFGTTSEIGIFEMKEGGLVSAKNASKLFFTQKSAQAGSAIAVILEGSRALVIEIQALVSESSYPK
RSCTGFDTNRLNMLLALLERKLELPLGRYDVFVNVAGGIKINEPSADLAVIASIISSFRNRPLNAKTAFIGEVSLTGDIR
EVSNIDTRLKELESYGFNKVILAKKPSNPSSTLKCFETNEVSRILEWM
>Mature_447_residues
AKKTSIFECQFCGWQSAKWLGKCSSCGSWDSLIELKPSQIKITKSLKSNPQNLSNAIPITQVSFENIERFSSFEEELDIV
LGGGIVPGGLYLIGGSPGVGKSTLLLKIAGEIAKHIHKKVLYVSGEESAGQIKMRASRLGAISEDLFLLNEIDLNIIQNA
LDEQQYALCVIDSIQTIYSPDITSAPGSISQVREVTFALMRLAKERNISIFIIGHITKEGSIAGPRILEHMVDCVLYFEG
DPSRELRMLRGFKNRFGTTSEIGIFEMKEGGLVSAKNASKLFFTQKSAQAGSAIAVILEGSRALVIEIQALVSESSYPKR
SCTGFDTNRLNMLLALLERKLELPLGRYDVFVNVAGGIKINEPSADLAVIASIISSFRNRPLNAKTAFIGEVSLTGDIRE
VSNIDTRLKELESYGFNKVILAKKPSNPSSTLKCFETNEVSRILEWM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=448, Percent_Identity=40.8482142857143, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49156; Mature: 49025

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTSIFECQFCGWQSAKWLGKCSSCGSWDSLIELKPSQIKITKSLKSNPQNLSNAIPI
CCCCCCEEEEEECCCCCCHHHHCCCCCCCCCHHEEECCCCEEEEHHHCCCCHHHHCCCCE
TQVSFENIERFSSFEEELDIVLGGGIVPGGLYLIGGSPGVGKSTLLLKIAGEIAKHIHKK
EEECHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHE
VLYVSGEESAGQIKMRASRLGAISEDLFLLNEIDLNIIQNALDEQQYALCVIDSIQTIYS
EEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHCC
PDITSAPGSISQVREVTFALMRLAKERNISIFIIGHITKEGSIAGPRILEHMVDCVLYFE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEEC
GDPSRELRMLRGFKNRFGTTSEIGIFEMKEGGLVSAKNASKLFFTQKSAQAGSAIAVILE
CCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCCCEEEEEEE
GSRALVIEIQALVSESSYPKRSCTGFDTNRLNMLLALLERKLELPLGRYDVFVNVAGGIK
CCCEEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEE
INEPSADLAVIASIISSFRNRPLNAKTAFIGEVSLTGDIREVSNIDTRLKELESYGFNKV
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEE
ILAKKPSNPSSTLKCFETNEVSRILEWM
EEEECCCCCCHHEEEECHHHHHHHHHCC
>Mature Secondary Structure 
AKKTSIFECQFCGWQSAKWLGKCSSCGSWDSLIELKPSQIKITKSLKSNPQNLSNAIPI
CCCCCEEEEEECCCCCCHHHHCCCCCCCCCHHEEECCCCEEEEHHHCCCCHHHHCCCCE
TQVSFENIERFSSFEEELDIVLGGGIVPGGLYLIGGSPGVGKSTLLLKIAGEIAKHIHKK
EEECHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHE
VLYVSGEESAGQIKMRASRLGAISEDLFLLNEIDLNIIQNALDEQQYALCVIDSIQTIYS
EEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHCC
PDITSAPGSISQVREVTFALMRLAKERNISIFIIGHITKEGSIAGPRILEHMVDCVLYFE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEEC
GDPSRELRMLRGFKNRFGTTSEIGIFEMKEGGLVSAKNASKLFFTQKSAQAGSAIAVILE
CCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCCCEEEEEEE
GSRALVIEIQALVSESSYPKRSCTGFDTNRLNMLLALLERKLELPLGRYDVFVNVAGGIK
CCCEEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEE
INEPSADLAVIASIISSFRNRPLNAKTAFIGEVSLTGDIREVSNIDTRLKELESYGFNKV
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEE
ILAKKPSNPSSTLKCFETNEVSRILEWM
EEEECCCCCCHHEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]