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The map label for this gene is tig

Identifier: 32266065

GI number: 32266065

Start: 544365

End: 545693

Strand: Direct

Name: tig

Synonym: HH0566

Alternate gene names: 32266065

Gene position: 544365-545693 (Clockwise)

Preceding gene: 32266061

Following gene: 32266066

Centisome position: 30.26

GC content: 31.9

Gene sequence:

>1329_bases
ATGAATTTCACAACAAAACGCATAAATAGCGCTAATGCTGTTATCAATGGTAGCATTGCACTCTCTAAAATTGAGGAAAA
ATTTGAAAAAGTCATCAAAAAAATTGCTAAAAATATAAAAATTGATGGCTTTAGAAAAGGTAAGGTCCCAACGCAAGTGA
TTAAAACACGTTATAAAGAGCAGATAGATCAAGATGCACAACAAGAAGCAATTCAAGAATTGCTTACCGCTGCACTTAAA
GAGCTAGAAATTCAGCCCAATAGCCTTATTGGCAATCCTATGATTTCTCAATTTAATAAGCTTAATGATAAAATAGAACT
TGAAATTAAGCTTGGCATTACCCCCACATTAAATCTTGATAATGTTGAGGATTACACACCTGAAGTCAAACTCAAAACAA
TAAGTAAAAATCTTATTGATGAGCGCTTAGAAGAAATTGCAAAAAATCGCGCACCTCTTAATGAAATCACTCAAGAGAGG
ACATTACAAAAAGATGATACAGCTCAAATTGATTTTGAAGGCTTTGTTGATGGAAAAGCATTTGAGGGTGGTAAAGGAGA
AAACTTCAATCTCGCTATTGGTTCAAATCAATTTATTCCGGGCTTTGAAGATGCACTCATCGGTATGAAAAATGGCGAGA
AGCGCACAATTAAGGTTACTTTCCCCGAGCAATATCAAGCTAAACATTTAGCAGGAAAAGAAGCAAGCTTTGATGTAACG
CTCCATAAGATTTTGCAAAAAGAGTTGCCAAAAATTGATGATGAGTTTGCAAAAAGCATTGCAGGCGAAGAATCTAACCT
ACAATCTTTAAAAGATATGATAAAAGAGCAACTTGAAATGGAACAAAAAACTGAAATTTACAATAAAGAACTCAAAGAAA
AATTAGTAGAAATATTACTTAAAAATATTTCATTTGATTTGCCAGATTTAATCGTTGAGCAAGAAATGGATATTTTATTT
AGAAATGCTCTTAGTCAGCTTAAACCAGAAGAATTTGATAAAATAAAAAACAATCAAGATGAAGCTAAAAAACAACGAGA
AACGCATAAAGATGAAGCACGCAAAAGTGTGCAAATTACTTTTATTATGGACGCTTTGGCAAAAAAATATAATATTGCCA
TTAATGATAATGAAGTATTACAAACTATTTATTATGAAGCTATGATGATGGGACAAGACCCAAAAGCAACACTAGAACAT
TATCAAAAAAACAATCTTGTTCCAGCTATTAAAATGACTATGCTTGAAGATAGAGTATTACACTATTTGCTTGATAAAAA
ATTTGAAGAAAGCAAGGCAAATACTAATGCTCAAAAGGATAATCAATGA

Upstream 100 bases:

>100_bases
GTTTTTGTTTGATTTGAGACAATGACATACACAAAACCTACTTTTAAGATTTTTTTGTTAGAATACCAAATTATACTTTT
TTATTTTATTAAGGAAAGCT

Downstream 100 bases:

>100_bases
GTAGTTATATTCCTTATGTCATTGAACGCACAGGACGCGGTGAGAGAAGCTATGATATTTATTCACGGCTTTTAAAAGAC
CGCATAATCTTACTAAGTGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNFTTKRINSANAVINGSIALSKIEEKFEKVIKKIAKNIKIDGFRKGKVPTQVIKTRYKEQIDQDAQQEAIQELLTAALK
ELEIQPNSLIGNPMISQFNKLNDKIELEIKLGITPTLNLDNVEDYTPEVKLKTISKNLIDERLEEIAKNRAPLNEITQER
TLQKDDTAQIDFEGFVDGKAFEGGKGENFNLAIGSNQFIPGFEDALIGMKNGEKRTIKVTFPEQYQAKHLAGKEASFDVT
LHKILQKELPKIDDEFAKSIAGEESNLQSLKDMIKEQLEMEQKTEIYNKELKEKLVEILLKNISFDLPDLIVEQEMDILF
RNALSQLKPEEFDKIKNNQDEAKKQRETHKDEARKSVQITFIMDALAKKYNIAINDNEVLQTIYYEAMMMGQDPKATLEH
YQKNNLVPAIKMTMLEDRVLHYLLDKKFEESKANTNAQKDNQ

Sequences:

>Translated_442_residues
MNFTTKRINSANAVINGSIALSKIEEKFEKVIKKIAKNIKIDGFRKGKVPTQVIKTRYKEQIDQDAQQEAIQELLTAALK
ELEIQPNSLIGNPMISQFNKLNDKIELEIKLGITPTLNLDNVEDYTPEVKLKTISKNLIDERLEEIAKNRAPLNEITQER
TLQKDDTAQIDFEGFVDGKAFEGGKGENFNLAIGSNQFIPGFEDALIGMKNGEKRTIKVTFPEQYQAKHLAGKEASFDVT
LHKILQKELPKIDDEFAKSIAGEESNLQSLKDMIKEQLEMEQKTEIYNKELKEKLVEILLKNISFDLPDLIVEQEMDILF
RNALSQLKPEEFDKIKNNQDEAKKQRETHKDEARKSVQITFIMDALAKKYNIAINDNEVLQTIYYEAMMMGQDPKATLEH
YQKNNLVPAIKMTMLEDRVLHYLLDKKFEESKANTNAQKDNQ
>Mature_442_residues
MNFTTKRINSANAVINGSIALSKIEEKFEKVIKKIAKNIKIDGFRKGKVPTQVIKTRYKEQIDQDAQQEAIQELLTAALK
ELEIQPNSLIGNPMISQFNKLNDKIELEIKLGITPTLNLDNVEDYTPEVKLKTISKNLIDERLEEIAKNRAPLNEITQER
TLQKDDTAQIDFEGFVDGKAFEGGKGENFNLAIGSNQFIPGFEDALIGMKNGEKRTIKVTFPEQYQAKHLAGKEASFDVT
LHKILQKELPKIDDEFAKSIAGEESNLQSLKDMIKEQLEMEQKTEIYNKELKEKLVEILLKNISFDLPDLIVEQEMDILF
RNALSQLKPEEFDKIKNNQDEAKKQRETHKDEARKSVQITFIMDALAKKYNIAINDNEVLQTIYYEAMMMGQDPKATLEH
YQKNNLVPAIKMTMLEDRVLHYLLDKKFEESKANTNAQKDNQ

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=411, Percent_Identity=29.9270072992701, Blast_Score=191, Evalue=9e-50,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_HELHP (Q7VIN8)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860097.1
- ProteinModelPortal:   Q7VIN8
- SMR:   Q7VIN8
- GeneID:   1493620
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0566
- NMPDR:   fig|235279.1.peg.566
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   HHEP235279:HH_0566-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 50711; Mature: 50711

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTTKRINSANAVINGSIALSKIEEKFEKVIKKIAKNIKIDGFRKGKVPTQVIKTRYKE
CCCCHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QIDQDAQQEAIQELLTAALKELEIQPNSLIGNPMISQFNKLNDKIELEIKLGITPTLNLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCC
NVEDYTPEVKLKTISKNLIDERLEEIAKNRAPLNEITQERTLQKDDTAQIDFEGFVDGKA
CCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCC
FEGGKGENFNLAIGSNQFIPGFEDALIGMKNGEKRTIKVTFPEQYQAKHLAGKEASFDVT
CCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCCCCHHHH
LHKILQKELPKIDDEFAKSIAGEESNLQSLKDMIKEQLEMEQKTEIYNKELKEKLVEILL
HHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNISFDLPDLIVEQEMDILFRNALSQLKPEEFDKIKNNQDEAKKQRETHKDEARKSVQIT
HHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHH
FIMDALAKKYNIAINDNEVLQTIYYEAMMMGQDPKATLEHYQKNNLVPAIKMTMLEDRVL
HHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
HYLLDKKFEESKANTNAQKDNQ
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFTTKRINSANAVINGSIALSKIEEKFEKVIKKIAKNIKIDGFRKGKVPTQVIKTRYKE
CCCCHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QIDQDAQQEAIQELLTAALKELEIQPNSLIGNPMISQFNKLNDKIELEIKLGITPTLNLD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCC
NVEDYTPEVKLKTISKNLIDERLEEIAKNRAPLNEITQERTLQKDDTAQIDFEGFVDGKA
CCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCC
FEGGKGENFNLAIGSNQFIPGFEDALIGMKNGEKRTIKVTFPEQYQAKHLAGKEASFDVT
CCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCCCCHHHH
LHKILQKELPKIDDEFAKSIAGEESNLQSLKDMIKEQLEMEQKTEIYNKELKEKLVEILL
HHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNISFDLPDLIVEQEMDILFRNALSQLKPEEFDKIKNNQDEAKKQRETHKDEARKSVQIT
HHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHH
FIMDALAKKYNIAINDNEVLQTIYYEAMMMGQDPKATLEHYQKNNLVPAIKMTMLEDRVL
HHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
HYLLDKKFEESKANTNAQKDNQ
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954