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The map label for this gene is hisH

Identifier: 32265984

GI number: 32265984

Start: 470499

End: 471152

Strand: Direct

Name: hisH

Synonym: HH0485

Alternate gene names: 32265984

Gene position: 470499-471152 (Clockwise)

Preceding gene: 32265983

Following gene: 32265985

Centisome position: 26.15

GC content: 37.31

Gene sequence:

>654_bases
ATGAGTAAGCTTCGTGTTGGTATTATTAATTATGGTGTAGGGAATCTCGGCAGTGTGCAAAATGCTTTTGCTTTTATACA
AACACATTTTAGCGATGTATTACCACACACACTTGAAATAAAGGTAGAATCTCACCCTGAAAGGCTACAAGATTACGATA
AACTCTTATTGCCGGGTGTTGGAGCATTTGGCAATGCAATGACACATTTAAAAAACACTCATTTAGATGAGGCTATCAGA
GAATTTGTCCAAAGCGGCAAATTCTTAATTGGGATTTGTTTAGGTATGCAACTGCTTTTTGAGCAAAGTGAAGAATTTGG
CAATCACAAAGGATTAGGACTTATTGAGGGCAAAGTAGTAGGATTTGAACATATTGCACCACTAAAAGTGCCACATATTG
GGTGGAATAGCTGTAATTTTACTCCTGAAGGCAAACATTCAAAGCTTTTTAGGGGTATTGCTGATGGAAGCTTTTTTTAT
TTCGTGCATAGCTTTCATATTCAAACACAACCTCAATTTATCCTTGCTCAATGCACCTATGGCTATCCTTTTGGTGCGAT
TGTCCATAAAGATAATCTTTTTGCCATACAAGCTCACCCCGAAAAAAGCCATAACGTAGGTTTAAGGCTTCTTGCAAATT
TCCTTACCTTATGA

Upstream 100 bases:

>100_bases
ACTCTCTCTTGTGTAGATTTAGAAAATGTGCGTGATAATCTCTTACAAAAAAGCAATGATGTAATTGCACAAAATAAACA
ATGGCTTTTAGGACTTGTCA

Downstream 100 bases:

>100_bases
AACATTGCTTTTCCATTTACCTAGTTGTTGTTTTAGTTAATTATAGTAATGGAAAATTTTTATTATTTGTAAGGAGAAAA
ATATGATTGATTTAGCAATT

Product: glutamine amidotransferase HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSKLRVGIINYGVGNLGSVQNAFAFIQTHFSDVLPHTLEIKVESHPERLQDYDKLLLPGVGAFGNAMTHLKNTHLDEAIR
EFVQSGKFLIGICLGMQLLFEQSEEFGNHKGLGLIEGKVVGFEHIAPLKVPHIGWNSCNFTPEGKHSKLFRGIADGSFFY
FVHSFHIQTQPQFILAQCTYGYPFGAIVHKDNLFAIQAHPEKSHNVGLRLLANFLTL

Sequences:

>Translated_217_residues
MSKLRVGIINYGVGNLGSVQNAFAFIQTHFSDVLPHTLEIKVESHPERLQDYDKLLLPGVGAFGNAMTHLKNTHLDEAIR
EFVQSGKFLIGICLGMQLLFEQSEEFGNHKGLGLIEGKVVGFEHIAPLKVPHIGWNSCNFTPEGKHSKLFRGIADGSFFY
FVHSFHIQTQPQFILAQCTYGYPFGAIVHKDNLFAIQAHPEKSHNVGLRLLANFLTL
>Mature_216_residues
SKLRVGIINYGVGNLGSVQNAFAFIQTHFSDVLPHTLEIKVESHPERLQDYDKLLLPGVGAFGNAMTHLKNTHLDEAIRE
FVQSGKFLIGICLGMQLLFEQSEEFGNHKGLGLIEGKVVGFEHIAPLKVPHIGWNSCNFTPEGKHSKLFRGIADGSFFYF
VHSFHIQTQPQFILAQCTYGYPFGAIVHKDNLFAIQAHPEKSHNVGLRLLANFLTL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=214, Percent_Identity=40.1869158878505, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=35.1351351351351, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_HELHP (Q7VIW9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860016.1
- ProteinModelPortal:   Q7VIW9
- GeneID:   1493539
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0485
- NMPDR:   fig|235279.1.peg.485
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- BioCyc:   HHEP235279:HH_0485-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 24227; Mature: 24096

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 93-93 ACT_SITE 199-199 ACT_SITE 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRVGIINYGVGNLGSVQNAFAFIQTHFSDVLPHTLEIKVESHPERLQDYDKLLLPGV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCC
GAFGNAMTHLKNTHLDEAIREFVQSGKFLIGICLGMQLLFEQSEEFGNHKGLGLIEGKVV
CHHHHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCEEE
GFEHIAPLKVPHIGWNSCNFTPEGKHSKLFRGIADGSFFYFVHSFHIQTQPQFILAQCTY
CHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECC
GYPFGAIVHKDNLFAIQAHPEKSHNVGLRLLANFLTL
CCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKLRVGIINYGVGNLGSVQNAFAFIQTHFSDVLPHTLEIKVESHPERLQDYDKLLLPGV
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCC
GAFGNAMTHLKNTHLDEAIREFVQSGKFLIGICLGMQLLFEQSEEFGNHKGLGLIEGKVV
CHHHHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCEEE
GFEHIAPLKVPHIGWNSCNFTPEGKHSKLFRGIADGSFFYFVHSFHIQTQPQFILAQCTY
CHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECC
GYPFGAIVHKDNLFAIQAHPEKSHNVGLRLLANFLTL
CCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954