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The map label for this gene is thrS

Identifier: 32265941

GI number: 32265941

Start: 422309

End: 424123

Strand: Direct

Name: thrS

Synonym: HH0442

Alternate gene names: 32265941

Gene position: 422309-424123 (Clockwise)

Preceding gene: 32265940

Following gene: 32265942

Centisome position: 23.47

GC content: 37.69

Gene sequence:

>1815_bases
ATGAGTGAAGTTATAGGCATTAAACACAATCAACATATTTATGACCTCTGCACAGCAGATGAACTTGGCATTACTGGGGA
TTCTATTAGTTTTGATAACTCTCAAGAATCTTTAGCAATTATTCGCCACTCTTGCGCACATCTAATGGCTCAAGCAATTA
AAATCCTCTATCCAGAAGCGCAATTTTTTGTTGGACCTGTCGTTGATGAGGGTTTTTATTACGATTTCAAAGTCAATACT
AAAATCAGTGAGGAAGATCTCCTTGTAATTGAAGCAAAAATGAAAGAGATTGCAAAAAATGCTTATCCTATCACTAAAAT
TACACTCTCACGCAAAGAAGCACAGGCACGATTTGCGCACGATGAACTTAAAGTAGCAGTAATGAGTCGTATCCCCGATG
AAAAACTAAGTATTTATACACAAGGTGATTTTGAGGATTTATGCAGGGGACCTCATCTCCCTAATACAAAACTTTTAGAA
CATTTCAAACTTACCAAAATTGCAGGGGCATATCTTGGCGGCGATGAAAATGCGCAAATGCTTATTAGAATTTATGGCAT
TGCCTTTGCCGATAAGCAATCACTCAAAGATTATCTATTCACACTTGAAGAAGCCAAAAAACGTGATCATCGCAAACTTG
GGCAAGAAATGGGACTTTTTACCTTTGATGAGGAAATTGGCGCAGGATTACCTATATGGCTACCAAAAGGAGCTAGATTA
AGACATAATATTGAGCATTTACTCACTCAAGCACTCAAAGAACGAGGCTATGAACCTGTAAGAGGACCAGAGATTCTCAA
AAGTGATGTATGGAAAAAAAGTGGACATTATAGCAATTACAAAGAAAATATGTATTTTACGATTATTGATGAACAAGAGT
ATGGCATTAAACCTATGAATTGTGTCGGGCATATTAAAGTCTATCAAAGCTCTCCACGAAGTTATAGAGAATTGCCACTG
CGTTTTTACGAATATGGCATAGTGCATAGACACGAACGAAGCGGTGTGTTACACGGGCTTTTGCGCGTGAGAGAATTTAC
TCAAGATGATGCACATATTTTTTGTATGACAAGTCAAATCAAGTCTGAAGTGAATCAGATTCTTGATTTTACAAAAGGCA
TTATGAATGCTTTTGGATTCCATTATGAAATGGAACTCTCCACACGTCCACAAAAATCTATTGGCAATGATGAAGTATGG
GAAAATGCTACAGCAGCACTCAAAAGTGCGCTTGAAGAAAATCACATAAGTTATCAAATTGATGAAGGAGGAGGAGCATT
CTATGGTCCTAAGATTGATATTAAAATCACTGATGCACTTAAACGCAAATGGCAATGTGGCACGATACAAATTGATATGA
ATCTCCCTGAACGCTTTGAGTTAAGCTATACAGATGAACATAATGTCCAAGTGCAGCCTGTTATGATTCATCGTGCAATT
CTTGGCTCATTTGAACGATTTGTGGCGATATTGACAGAGCATTTTGGGGGTGAGTTTCCGTTATTTATCGCACCTACACA
AGTGATTTTAATTCCTATTGGTGAAGCGCAACTTGAATATGCAAGAATCTTGCGTGATAAAATCATTACTCAAAGTGGTG
CATATGCCGAACTTATGGATAAGAATGAAAGCTTAAGTAAAAAAATACGACTTGCTGAAAAGCAGCGTGTGCCTTTGATT
GTTGTTATCGGGGCAAAAGAAGTGGAGAGCAAAATCCTTGCAATCCGAGATAGGCGGGAGAAATCACAATATGAGCTCCC
TGAAGAAGCATTCATTGCTACATTGAAAACAAAAATAGGAGAGGTTAGTTTTTGA

Upstream 100 bases:

>100_bases
AAAAAGCACTTGAAGAAATTCAAAGATATACCGATGAATTTGTCAAAAAATGCGATGAAATGCTCAAACACAAAGAAGAA
GAGGTGATGAAAGTATAGCA

Downstream 100 bases:

>100_bases
GCAAAGAAGAAGTATTACTCAATGAGGAGATAGATTTTAAGGAAGTGCGCTGCGTAAGTGATAATGGCGAGGTATACGGC
ATTATTTCATCTAAAGAAGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MSEVIGIKHNQHIYDLCTADELGITGDSISFDNSQESLAIIRHSCAHLMAQAIKILYPEAQFFVGPVVDEGFYYDFKVNT
KISEEDLLVIEAKMKEIAKNAYPITKITLSRKEAQARFAHDELKVAVMSRIPDEKLSIYTQGDFEDLCRGPHLPNTKLLE
HFKLTKIAGAYLGGDENAQMLIRIYGIAFADKQSLKDYLFTLEEAKKRDHRKLGQEMGLFTFDEEIGAGLPIWLPKGARL
RHNIEHLLTQALKERGYEPVRGPEILKSDVWKKSGHYSNYKENMYFTIIDEQEYGIKPMNCVGHIKVYQSSPRSYRELPL
RFYEYGIVHRHERSGVLHGLLRVREFTQDDAHIFCMTSQIKSEVNQILDFTKGIMNAFGFHYEMELSTRPQKSIGNDEVW
ENATAALKSALEENHISYQIDEGGGAFYGPKIDIKITDALKRKWQCGTIQIDMNLPERFELSYTDEHNVQVQPVMIHRAI
LGSFERFVAILTEHFGGEFPLFIAPTQVILIPIGEAQLEYARILRDKIITQSGAYAELMDKNESLSKKIRLAEKQRVPLI
VVIGAKEVESKILAIRDRREKSQYELPEEAFIATLKTKIGEVSF

Sequences:

>Translated_604_residues
MSEVIGIKHNQHIYDLCTADELGITGDSISFDNSQESLAIIRHSCAHLMAQAIKILYPEAQFFVGPVVDEGFYYDFKVNT
KISEEDLLVIEAKMKEIAKNAYPITKITLSRKEAQARFAHDELKVAVMSRIPDEKLSIYTQGDFEDLCRGPHLPNTKLLE
HFKLTKIAGAYLGGDENAQMLIRIYGIAFADKQSLKDYLFTLEEAKKRDHRKLGQEMGLFTFDEEIGAGLPIWLPKGARL
RHNIEHLLTQALKERGYEPVRGPEILKSDVWKKSGHYSNYKENMYFTIIDEQEYGIKPMNCVGHIKVYQSSPRSYRELPL
RFYEYGIVHRHERSGVLHGLLRVREFTQDDAHIFCMTSQIKSEVNQILDFTKGIMNAFGFHYEMELSTRPQKSIGNDEVW
ENATAALKSALEENHISYQIDEGGGAFYGPKIDIKITDALKRKWQCGTIQIDMNLPERFELSYTDEHNVQVQPVMIHRAI
LGSFERFVAILTEHFGGEFPLFIAPTQVILIPIGEAQLEYARILRDKIITQSGAYAELMDKNESLSKKIRLAEKQRVPLI
VVIGAKEVESKILAIRDRREKSQYELPEEAFIATLKTKIGEVSF
>Mature_603_residues
SEVIGIKHNQHIYDLCTADELGITGDSISFDNSQESLAIIRHSCAHLMAQAIKILYPEAQFFVGPVVDEGFYYDFKVNTK
ISEEDLLVIEAKMKEIAKNAYPITKITLSRKEAQARFAHDELKVAVMSRIPDEKLSIYTQGDFEDLCRGPHLPNTKLLEH
FKLTKIAGAYLGGDENAQMLIRIYGIAFADKQSLKDYLFTLEEAKKRDHRKLGQEMGLFTFDEEIGAGLPIWLPKGARLR
HNIEHLLTQALKERGYEPVRGPEILKSDVWKKSGHYSNYKENMYFTIIDEQEYGIKPMNCVGHIKVYQSSPRSYRELPLR
FYEYGIVHRHERSGVLHGLLRVREFTQDDAHIFCMTSQIKSEVNQILDFTKGIMNAFGFHYEMELSTRPQKSIGNDEVWE
NATAALKSALEENHISYQIDEGGGAFYGPKIDIKITDALKRKWQCGTIQIDMNLPERFELSYTDEHNVQVQPVMIHRAIL
GSFERFVAILTEHFGGEFPLFIAPTQVILIPIGEAQLEYARILRDKIITQSGAYAELMDKNESLSKKIRLAEKQRVPLIV
VIGAKEVESKILAIRDRREKSQYELPEEAFIATLKTKIGEVSF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=577, Percent_Identity=38.474870017331, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI38570111, Length=600, Percent_Identity=37, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI20070344, Length=610, Percent_Identity=34.5901639344262, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1788013, Length=577, Percent_Identity=45.580589254766, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1786392, Length=136, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984192, Length=592, Percent_Identity=37.8378378378378, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984184, Length=592, Percent_Identity=37.8378378378378, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322112, Length=561, Percent_Identity=40.6417112299465, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322654, Length=404, Percent_Identity=37.3762376237624, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24583842, Length=597, Percent_Identity=38.8609715242881, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI28574141, Length=597, Percent_Identity=38.8609715242881, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24583839, Length=597, Percent_Identity=38.8609715242881, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_HELHP (Q7VJ09)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_859973.1
- ProteinModelPortal:   Q7VJ09
- SMR:   Q7VJ09
- GeneID:   1493496
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0442
- NMPDR:   fig|235279.1.peg.442
- HOGENOM:   HBG352811
- OMA:   EVIRHSC
- ProtClustDB:   PRK12305
- BioCyc:   HHEP235279:HH_0442-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 69118; Mature: 68987

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVIGIKHNQHIYDLCTADELGITGDSISFDNSQESLAIIRHSCAHLMAQAIKILYPEA
CCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
QFFVGPVVDEGFYYDFKVNTKISEEDLLVIEAKMKEIAKNAYPITKITLSRKEAQARFAH
HHEECCEECCCEEEEEEECCEECCCCEEEEEHHHHHHHHCCCCEEEEEECHHHHHHHHHH
DELKVAVMSRIPDEKLSIYTQGDFEDLCRGPHLPNTKLLEHFKLTKIAGAYLGGDENAQM
HHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
LIRIYGIAFADKQSLKDYLFTLEEAKKRDHRKLGQEMGLFTFDEEIGAGLPIWLPKGARL
EEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCEECCCCCHH
RHNIEHLLTQALKERGYEPVRGPEILKSDVWKKSGHYSNYKENMYFTIIDEQEYGIKPMN
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCC
CVGHIKVYQSSPRSYRELPLRFYEYGIVHRHERSGVLHGLLRVREFTQDDAHIFCMTSQI
CEEEEEEEECCCCHHHHCCHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHH
KSEVNQILDFTKGIMNAFGFHYEMELSTRPQKSIGNDEVWENATAALKSALEENHISYQI
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
DEGGGAFYGPKIDIKITDALKRKWQCGTIQIDMNLPERFELSYTDEHNVQVQPVMIHRAI
ECCCCEEECCEEEEEEEHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCEEEHHHHHHHH
LGSFERFVAILTEHFGGEFPLFIAPTQVILIPIGEAQLEYARILRDKIITQSGAYAELMD
HHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHC
KNESLSKKIRLAEKQRVPLIVVIGAKEVESKILAIRDRREKSQYELPEEAFIATLKTKIG
CCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
EVSF
CCCC
>Mature Secondary Structure 
SEVIGIKHNQHIYDLCTADELGITGDSISFDNSQESLAIIRHSCAHLMAQAIKILYPEA
CCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
QFFVGPVVDEGFYYDFKVNTKISEEDLLVIEAKMKEIAKNAYPITKITLSRKEAQARFAH
HHEECCEECCCEEEEEEECCEECCCCEEEEEHHHHHHHHCCCCEEEEEECHHHHHHHHHH
DELKVAVMSRIPDEKLSIYTQGDFEDLCRGPHLPNTKLLEHFKLTKIAGAYLGGDENAQM
HHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
LIRIYGIAFADKQSLKDYLFTLEEAKKRDHRKLGQEMGLFTFDEEIGAGLPIWLPKGARL
EEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCEECCCCCHH
RHNIEHLLTQALKERGYEPVRGPEILKSDVWKKSGHYSNYKENMYFTIIDEQEYGIKPMN
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCC
CVGHIKVYQSSPRSYRELPLRFYEYGIVHRHERSGVLHGLLRVREFTQDDAHIFCMTSQI
CEEEEEEEECCCCHHHHCCHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHH
KSEVNQILDFTKGIMNAFGFHYEMELSTRPQKSIGNDEVWENATAALKSALEENHISYQI
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
DEGGGAFYGPKIDIKITDALKRKWQCGTIQIDMNLPERFELSYTDEHNVQVQPVMIHRAI
ECCCCEEECCEEEEEEEHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCEEEHHHHHHHH
LGSFERFVAILTEHFGGEFPLFIAPTQVILIPIGEAQLEYARILRDKIITQSGAYAELMD
HHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHC
KNESLSKKIRLAEKQRVPLIVVIGAKEVESKILAIRDRREKSQYELPEEAFIATLKTKIG
CCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
EVSF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954