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The map label for this gene is birA

Identifier: 32265920

GI number: 32265920

Start: 406464

End: 407183

Strand: Direct

Name: birA

Synonym: HH0421

Alternate gene names: 32265920

Gene position: 406464-407183 (Clockwise)

Preceding gene: 32265919

Following gene: 32265921

Centisome position: 22.59

GC content: 33.19

Gene sequence:

>720_bases
ATGACTCATAATTCACCACAAGCTTTTTTTACAACTTCTTTTATCACTTTGCTCCAAGAACATATATACCATTTTGATAC
ATTACCCTCTACCCAAACTTGGGCTGTTGAAAAAATTAAAGCAAAAACGCTTATAGCACCTTTTTGTATCCACGCTAAAA
GACAAACACAAGGTATAGGAAGCCGAGGCAACCAATGGGAAAATGTGCCACGAGGGCTTATGTTTTCATTTGCACTACCA
CTAGTCAGCTTACCGCAAGATTTAAAGATAGAATCAAGTTCCATATTTTTTGGTGTCATTTTACAACAATTTCTTGCTTC
ACTTGGCTCAAAAGTGTGGCTCAAATATCCTAATGATTTATACATAAATGAGCACAAAATTGGCGGTATTCTTACGCAAA
AAATACACGATAGTCTTGTATGCGGTATGGGTATAAACCTTTATCATTCTCCCGATGCTTCAAATAGCCAACACTACGCT
ACTCTAGAAAAATCTATCAGTGCTAATATAGAACCTTTAAGCTTTTTGAGCAATTTTTTTCAAAGCTTTGAAAAATTTAT
ATCTTGGAAGCAGATTTTTAGTATTTATAAGTTAGAATTTCACAAAAATAACTCTTATTTTTTTCACTTTCAGAATAAAC
ACATTTGTCTGAAAGACACAATACTCAATGAAGATGGCTCTTTGAGTATTGATGGACATAAAATTTATAGCTTACGATAA

Upstream 100 bases:

>100_bases
ATTGCAACAAGAGGGAAAAGAAAAGCTCAATGCCGCACTTTATTTACGTGGCAAAAGACTTAATGTAGGCGATATATTGT
GCTAAAATAAGGTATTATCA

Downstream 100 bases:

>100_bases
AGGGGAGACAATGAGTGAAATTATAACCATAGCAAACCAAAAAGGTGGCGTAGGCAAAACAACTACCACTGTGAATCTAG
CAGCCTTTTTGGCTTCAGCA

Product: biotin--protein ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Alternate protein names: Biotin/Acetyl-CoA-Carboxylase Ligase; Biotin-[Acetyl-CoA-Carboxylase] Synthetase; Biotin-Acetyl-CoA-Carboxylase Ligase; Biotin-[Acetyl-CoA-Carboxylase] Ligase; Biotin-(Acetyl-CoA-Carboxylase) Ligase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTHNSPQAFFTTSFITLLQEHIYHFDTLPSTQTWAVEKIKAKTLIAPFCIHAKRQTQGIGSRGNQWENVPRGLMFSFALP
LVSLPQDLKIESSSIFFGVILQQFLASLGSKVWLKYPNDLYINEHKIGGILTQKIHDSLVCGMGINLYHSPDASNSQHYA
TLEKSISANIEPLSFLSNFFQSFEKFISWKQIFSIYKLEFHKNNSYFFHFQNKHICLKDTILNEDGSLSIDGHKIYSLR

Sequences:

>Translated_239_residues
MTHNSPQAFFTTSFITLLQEHIYHFDTLPSTQTWAVEKIKAKTLIAPFCIHAKRQTQGIGSRGNQWENVPRGLMFSFALP
LVSLPQDLKIESSSIFFGVILQQFLASLGSKVWLKYPNDLYINEHKIGGILTQKIHDSLVCGMGINLYHSPDASNSQHYA
TLEKSISANIEPLSFLSNFFQSFEKFISWKQIFSIYKLEFHKNNSYFFHFQNKHICLKDTILNEDGSLSIDGHKIYSLR
>Mature_238_residues
THNSPQAFFTTSFITLLQEHIYHFDTLPSTQTWAVEKIKAKTLIAPFCIHAKRQTQGIGSRGNQWENVPRGLMFSFALPL
VSLPQDLKIESSSIFFGVILQQFLASLGSKVWLKYPNDLYINEHKIGGILTQKIHDSLVCGMGINLYHSPDASNSQHYAT
LEKSISANIEPLSFLSNFFQSFEKFISWKQIFSIYKLEFHKNNSYFFHFQNKHICLKDTILNEDGSLSIDGHKIYSLR

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15

Molecular weight: Translated: 27427; Mature: 27296

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHNSPQAFFTTSFITLLQEHIYHFDTLPSTQTWAVEKIKAKTLIAPFCIHAKRQTQGIG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRGNQWENVPRGLMFSFALPLVSLPQDLKIESSSIFFGVILQQFLASLGSKVWLKYPNDL
CCCCCCHHCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEECCCEE
YINEHKIGGILTQKIHDSLVCGMGINLYHSPDASNSQHYATLEKSISANIEPLSFLSNFF
EEECCHHCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QSFEKFISWKQIFSIYKLEFHKNNSYFFHFQNKHICLKDTILNEDGSLSIDGHKIYSLR
HHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCEEEEEEEEECCCCCEEECCEEEEEEC
>Mature Secondary Structure 
THNSPQAFFTTSFITLLQEHIYHFDTLPSTQTWAVEKIKAKTLIAPFCIHAKRQTQGIG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRGNQWENVPRGLMFSFALPLVSLPQDLKIESSSIFFGVILQQFLASLGSKVWLKYPNDL
CCCCCCHHCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEECCCEE
YINEHKIGGILTQKIHDSLVCGMGINLYHSPDASNSQHYATLEKSISANIEPLSFLSNFF
EEECCHHCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QSFEKFISWKQIFSIYKLEFHKNNSYFFHFQNKHICLKDTILNEDGSLSIDGHKIYSLR
HHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCEEEEEEEEECCCCCEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA