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The map label for this gene is ispA

Identifier: 32265833

GI number: 32265833

Start: 329731

End: 330597

Strand: Direct

Name: ispA

Synonym: HH0334

Alternate gene names: 32265833

Gene position: 329731-330597 (Clockwise)

Preceding gene: 32265832

Following gene: 32265834

Centisome position: 18.33

GC content: 36.56

Gene sequence:

>867_bases
ATGAGTATTTCACAGATTCTTAATGATTTTGAATCCTTTCTTATCAGTCAAGCACCTCAGATTCCAAGTTTTCACCCACA
TTATGAACAAGCTGTGTGGGAAATGATGAAAAATGGTGGCAAAAGATTCCGCCCTGCACTTGTATTTTGTGTAGTTAATG
CTCTTGCTCCTCAACTGATTAAAAATGCTTTTTTACCTGCTCTAAGCATTGAATGTATCCATACTTATTCGCTTATACAC
GATGATTTGCCTTGTATGGATAATGCACCTTTAAGGCGTGGACACGCTACATTGCACACAAAATATGACGAAACACTTGC
GCTTTTAGTAGGTGATGGACTAAACACCTATGCTTTTGCTTTGCTTTCTCAAGCACGACTTGACGCGCAAGTGCGCTTAG
AGCTTATCCATTCTCTTGCACTCAATGCTGGTATTGGTGGTATGGTGCTTGGACAAATGCTTGATTGTGCTTTTGAGGAT
AAAAAACTTTCATTACAAGAACTTCAAATTATACACACAAACAAAACTGCTAAACTCATTGCTACTTCTTTGCAATTTGG
TGCTATTATTTCAAATGCAAAAGCACATTTTGCTTCATCAATTTATGAGTTTGGGCTAAAGCTTGGCATATATTTTCAGC
TGCGTGATGATCTTATTGATGCCTGTTTTGATGCACAACAAGCAGGTAAAACGACACACAATGATATACATAAAAATAGT
TATGTGAATCTACTTGGCTTAGAAGGTGCAAAAGTGGAATTTACTCGTATGAAAGAGCAGATTCAAAAAGAACTCCAAAC
ATTTGATGAAGCCATTTATGTTCATTTGACTACTCTTTTGCAAGATTATTTCAAGGATATTGAATGA

Upstream 100 bases:

>100_bases
TCCTCTCTCAAACACAATTTGATGAAAGATTTGAAGGCAAAATGCAACTCCTTATTGTGCGTGATAATCTTGAGATATTC
GTTAAAGTCTAGGAGCGAGT

Downstream 100 bases:

>100_bases
AGCGCATTCTCCTTACCAATGATGATGGCTTTGACTCAAGCGGTCTTCTTGCACTCAAAGATGCCCTCAAAGATATAGCA
CACGTGATGGTTGTCGCTCC

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSISQILNDFESFLISQAPQIPSFHPHYEQAVWEMMKNGGKRFRPALVFCVVNALAPQLIKNAFLPALSIECIHTYSLIH
DDLPCMDNAPLRRGHATLHTKYDETLALLVGDGLNTYAFALLSQARLDAQVRLELIHSLALNAGIGGMVLGQMLDCAFED
KKLSLQELQIIHTNKTAKLIATSLQFGAIISNAKAHFASSIYEFGLKLGIYFQLRDDLIDACFDAQQAGKTTHNDIHKNS
YVNLLGLEGAKVEFTRMKEQIQKELQTFDEAIYVHLTTLLQDYFKDIE

Sequences:

>Translated_288_residues
MSISQILNDFESFLISQAPQIPSFHPHYEQAVWEMMKNGGKRFRPALVFCVVNALAPQLIKNAFLPALSIECIHTYSLIH
DDLPCMDNAPLRRGHATLHTKYDETLALLVGDGLNTYAFALLSQARLDAQVRLELIHSLALNAGIGGMVLGQMLDCAFED
KKLSLQELQIIHTNKTAKLIATSLQFGAIISNAKAHFASSIYEFGLKLGIYFQLRDDLIDACFDAQQAGKTTHNDIHKNS
YVNLLGLEGAKVEFTRMKEQIQKELQTFDEAIYVHLTTLLQDYFKDIE
>Mature_287_residues
SISQILNDFESFLISQAPQIPSFHPHYEQAVWEMMKNGGKRFRPALVFCVVNALAPQLIKNAFLPALSIECIHTYSLIHD
DLPCMDNAPLRRGHATLHTKYDETLALLVGDGLNTYAFALLSQARLDAQVRLELIHSLALNAGIGGMVLGQMLDCAFEDK
KLSLQELQIIHTNKTAKLIATSLQFGAIISNAKAHFASSIYEFGLKLGIYFQLRDDLIDACFDAQQAGKTTHNDIHKNSY
VNLLGLEGAKVEFTRMKEQIQKELQTFDEAIYVHLTTLLQDYFKDIE

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=206, Percent_Identity=29.6116504854369, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786623, Length=256, Percent_Identity=37.109375, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789578, Length=204, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=248, Percent_Identity=30.6451612903226, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24651612, Length=223, Percent_Identity=26.457399103139, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32329; Mature: 32197

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISQILNDFESFLISQAPQIPSFHPHYEQAVWEMMKNGGKRFRPALVFCVVNALAPQLI
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KNAFLPALSIECIHTYSLIHDDLPCMDNAPLRRGHATLHTKYDETLALLVGDGLNTYAFA
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECHHHEEEEEECCCCHHHHHH
LLSQARLDAQVRLELIHSLALNAGIGGMVLGQMLDCAFEDKKLSLQELQIIHTNKTAKLI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHH
ATSLQFGAIISNAKAHFASSIYEFGLKLGIYFQLRDDLIDACFDAQQAGKTTHNDIHKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCCC
YVNLLGLEGAKVEFTRMKEQIQKELQTFDEAIYVHLTTLLQDYFKDIE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SISQILNDFESFLISQAPQIPSFHPHYEQAVWEMMKNGGKRFRPALVFCVVNALAPQLI
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KNAFLPALSIECIHTYSLIHDDLPCMDNAPLRRGHATLHTKYDETLALLVGDGLNTYAFA
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECHHHEEEEEECCCCHHHHHH
LLSQARLDAQVRLELIHSLALNAGIGGMVLGQMLDCAFEDKKLSLQELQIIHTNKTAKLI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHH
ATSLQFGAIISNAKAHFASSIYEFGLKLGIYFQLRDDLIDACFDAQQAGKTTHNDIHKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCCC
YVNLLGLEGAKVEFTRMKEQIQKELQTFDEAIYVHLTTLLQDYFKDIE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]