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The map label for this gene is hydA [H]

Identifier: 32265697

GI number: 32265697

Start: 190571

End: 192229

Strand: Direct

Name: hydA [H]

Synonym: HH0198

Alternate gene names: 32265697

Gene position: 190571-192229 (Clockwise)

Preceding gene: 32265696

Following gene: 32265698

Centisome position: 10.59

GC content: 39.06

Gene sequence:

>1659_bases
ATGGATAAAACACAGATACTCGAGCGATTAAATATACATCTTAACACTCTCAAAATCACATATAAAGAATCTCGCCATAA
AGTAAATGTGCAATGGGTATCCTACATCTGCTCTCTTATAGGCATTCCCAAAGAAGGTGTAGAGCTATGTGCTCAAATCC
TTTCTCTCAAACCACCTACACGCCTTGTGTGGCTTCATTTAGCAGAATGCACGGGTTGTAGCAGTAGTTTTTTGCGCCTT
GATAAGCCCGATATAGAATCTGTGCTTCTTGAATACATTACTCTTGAATACCACGAAACAATTATGGGGGCAGTAGGCTT
TAGCGCGAAAAAATCATTACATAATACTTTGGATAAAGAGTTTATCCTCGTTATTGAAGGTGGTGTATCACTAGGAAATC
ACGCACATTTTCTTACCTCTGGTGCAGATAGTATCACGGGCGAAGAAGAATGTAAAGAAGTAGCACAAAAGGCTCAAGCA
ATATTCGCAGTAGGCACTTGCTCAAGCTTTGGCGGGGTACAAGCAGCATATCCTAATCCTACGCAATCTATAGGGATAGA
TTCATTTCTTTCTCAACGCATTATCAACATACCCGGCTGTCCGCCAAGTGAGGCAAATATTATCGGTAGTATTCTCTATT
ATATCCTTTTGCAAGAGCTTCCCCCAACAGATTCTCTTCATCGTCCAATATGGAGCTATGGAAAGAATCTACACGATATG
TGTGAGCGCAAAGCAAAGTTTGAATCTGGTGATTTTGTGCAGAGCTTTGATGACCCGCATTTGGAAGATGGCTATTGTCT
TTATAAAGTCGGGTGTAAAGGACCATATGTTTTTAATAACTGCCCAAAGGTTAAATTTAATGCTAAAACAAGTTATCCTA
TTCAAGCAGGACACGGCTGTATTGCGTGTAGCGAGCCTGATTTTTGGGATAGCTTTGGACGCATAGAAGAGCCACTGAAT
AACTCGAATGCTTATCTTAGGCAGTCAAAAACATTACAGAATCTACCGCACATTCATAATCTTAAAGAATCTTTGGGTGA
GAGAATATTGTGCCTTGATCTTGATACACTCTCTCCCACTCGCATATACACGCAATCATCGCAAAGTAACCTCCTCACAC
TTTGCTTTCAATCTCATCTCCCTACTCTGCTCGCTCAAATTGCAAAAAGAAATAAACTAGGAGCACGGCTTGTAGAAAAT
TATAAACAATGGCGAGTACAGCAGCAACTCCCTGATATAAACATAAATCAAGACTTTCAAGGTGAGCCTTCTCACAATGT
AAGTGATATTTTAAAGCTCATTGCTGAAATGTTTGGTAAGAGCAACCCTAATGCACTTGATACCCTTGAAAATGCACAAA
ACTATCTCTTTACTCATATAAGTAAGCTTGATATGAAACTCAATGGTAAAGAAGTCTGCAATATTGATATAGATAAATCT
CTGCGCTTACCTTTGTGCTATCTACTTGGAGGATTAGAGATTGAAGGTGTAGCCTATGGTGTGGTAAGCTCTATATGTGA
GATTCTATCCCTTAGCCTCGTAGAACTATGCAAATATTATGACTTAGAACAAGTGGCATTTAAAGGAAATCTAATGGAAA
ATACTCTTATACAGGATAGATTTGCCAACTATCTCCCAAAGTGGATTCAAGTAGTATAA

Upstream 100 bases:

>100_bases
AAACCTGCAAATAATACTATGGCAACAAAAAGTTAGGCTAATTTTACCTAGCTTTAAATAGAGTAAGCTACAATAGCTTT
TTTACACTATGGAGTTAGCT

Downstream 100 bases:

>100_bases
ATCACAAGTGGAGGAGAAACCATAGACATTAATAGTAGAAATCCAAAGCTTAATATTAAAATACATATTGATAAGGGGAT
ATAAGATGAAATGCAAATTT

Product: hypothetical protein

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MDKTQILERLNIHLNTLKITYKESRHKVNVQWVSYICSLIGIPKEGVELCAQILSLKPPTRLVWLHLAECTGCSSSFLRL
DKPDIESVLLEYITLEYHETIMGAVGFSAKKSLHNTLDKEFILVIEGGVSLGNHAHFLTSGADSITGEEECKEVAQKAQA
IFAVGTCSSFGGVQAAYPNPTQSIGIDSFLSQRIINIPGCPPSEANIIGSILYYILLQELPPTDSLHRPIWSYGKNLHDM
CERKAKFESGDFVQSFDDPHLEDGYCLYKVGCKGPYVFNNCPKVKFNAKTSYPIQAGHGCIACSEPDFWDSFGRIEEPLN
NSNAYLRQSKTLQNLPHIHNLKESLGERILCLDLDTLSPTRIYTQSSQSNLLTLCFQSHLPTLLAQIAKRNKLGARLVEN
YKQWRVQQQLPDININQDFQGEPSHNVSDILKLIAEMFGKSNPNALDTLENAQNYLFTHISKLDMKLNGKEVCNIDIDKS
LRLPLCYLLGGLEIEGVAYGVVSSICEILSLSLVELCKYYDLEQVAFKGNLMENTLIQDRFANYLPKWIQVV

Sequences:

>Translated_552_residues
MDKTQILERLNIHLNTLKITYKESRHKVNVQWVSYICSLIGIPKEGVELCAQILSLKPPTRLVWLHLAECTGCSSSFLRL
DKPDIESVLLEYITLEYHETIMGAVGFSAKKSLHNTLDKEFILVIEGGVSLGNHAHFLTSGADSITGEEECKEVAQKAQA
IFAVGTCSSFGGVQAAYPNPTQSIGIDSFLSQRIINIPGCPPSEANIIGSILYYILLQELPPTDSLHRPIWSYGKNLHDM
CERKAKFESGDFVQSFDDPHLEDGYCLYKVGCKGPYVFNNCPKVKFNAKTSYPIQAGHGCIACSEPDFWDSFGRIEEPLN
NSNAYLRQSKTLQNLPHIHNLKESLGERILCLDLDTLSPTRIYTQSSQSNLLTLCFQSHLPTLLAQIAKRNKLGARLVEN
YKQWRVQQQLPDININQDFQGEPSHNVSDILKLIAEMFGKSNPNALDTLENAQNYLFTHISKLDMKLNGKEVCNIDIDKS
LRLPLCYLLGGLEIEGVAYGVVSSICEILSLSLVELCKYYDLEQVAFKGNLMENTLIQDRFANYLPKWIQVV
>Mature_552_residues
MDKTQILERLNIHLNTLKITYKESRHKVNVQWVSYICSLIGIPKEGVELCAQILSLKPPTRLVWLHLAECTGCSSSFLRL
DKPDIESVLLEYITLEYHETIMGAVGFSAKKSLHNTLDKEFILVIEGGVSLGNHAHFLTSGADSITGEEECKEVAQKAQA
IFAVGTCSSFGGVQAAYPNPTQSIGIDSFLSQRIINIPGCPPSEANIIGSILYYILLQELPPTDSLHRPIWSYGKNLHDM
CERKAKFESGDFVQSFDDPHLEDGYCLYKVGCKGPYVFNNCPKVKFNAKTSYPIQAGHGCIACSEPDFWDSFGRIEEPLN
NSNAYLRQSKTLQNLPHIHNLKESLGERILCLDLDTLSPTRIYTQSSQSNLLTLCFQSHLPTLLAQIAKRNKLGARLVEN
YKQWRVQQQLPDININQDFQGEPSHNVSDILKLIAEMFGKSNPNALDTLENAQNYLFTHISKLDMKLNGKEVCNIDIDKS
LRLPLCYLLGGLEIEGVAYGVVSSICEILSLSLVELCKYYDLEQVAFKGNLMENTLIQDRFANYLPKWIQVV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=302, Percent_Identity=40.0662251655629, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789371, Length=254, Percent_Identity=36.2204724409449, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 61945; Mature: 61945

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTQILERLNIHLNTLKITYKESRHKVNVQWVSYICSLIGIPKEGVELCAQILSLKPPT
CCHHHHHHHHCCEEEEEEEEEECCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
RLVWLHLAECTGCSSSFLRLDKPDIESVLLEYITLEYHETIMGAVGFSAKKSLHNTLDKE
CEEEEEEHHHCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCE
FILVIEGGVSLGNHAHFLTSGADSITGEEECKEVAQKAQAIFAVGTCSSFGGVQAAYPNP
EEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCC
TQSIGIDSFLSQRIINIPGCPPSEANIIGSILYYILLQELPPTDSLHRPIWSYGKNLHDM
CHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHCCCHHHH
CERKAKFESGDFVQSFDDPHLEDGYCLYKVGCKGPYVFNNCPKVKFNAKTSYPIQAGHGC
HHHHHCCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCEECCCCE
IACSEPDFWDSFGRIEEPLNNSNAYLRQSKTLQNLPHIHNLKESLGERILCLDLDTLSPT
EECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCC
RIYTQSSQSNLLTLCFQSHLPTLLAQIAKRNKLGARLVENYKQWRVQQQLPDININQDFQ
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEPSHNVSDILKLIAEMFGKSNPNALDTLENAQNYLFTHISKLDMKLNGKEVCNIDIDKS
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEECCCC
LRLPLCYLLGGLEIEGVAYGVVSSICEILSLSLVELCKYYDLEQVAFKGNLMENTLIQDR
CCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHH
FANYLPKWIQVV
HHHHHHHHHHCC
>Mature Secondary Structure
MDKTQILERLNIHLNTLKITYKESRHKVNVQWVSYICSLIGIPKEGVELCAQILSLKPPT
CCHHHHHHHHCCEEEEEEEEEECCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
RLVWLHLAECTGCSSSFLRLDKPDIESVLLEYITLEYHETIMGAVGFSAKKSLHNTLDKE
CEEEEEEHHHCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCE
FILVIEGGVSLGNHAHFLTSGADSITGEEECKEVAQKAQAIFAVGTCSSFGGVQAAYPNP
EEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCC
TQSIGIDSFLSQRIINIPGCPPSEANIIGSILYYILLQELPPTDSLHRPIWSYGKNLHDM
CHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHCCCHHHH
CERKAKFESGDFVQSFDDPHLEDGYCLYKVGCKGPYVFNNCPKVKFNAKTSYPIQAGHGC
HHHHHCCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEECCCCCEEECCCCCCCEECCCCE
IACSEPDFWDSFGRIEEPLNNSNAYLRQSKTLQNLPHIHNLKESLGERILCLDLDTLSPT
EECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCC
RIYTQSSQSNLLTLCFQSHLPTLLAQIAKRNKLGARLVENYKQWRVQQQLPDININQDFQ
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEPSHNVSDILKLIAEMFGKSNPNALDTLENAQNYLFTHISKLDMKLNGKEVCNIDIDKS
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEECCCC
LRLPLCYLLGGLEIEGVAYGVVSSICEILSLSLVELCKYYDLEQVAFKGNLMENTLIQDR
CCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHH
FANYLPKWIQVV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]