Click here to switch to the map view.

The map label for this gene is gmd

Identifier: 32265671

GI number: 32265671

Start: 165492

End: 166628

Strand: Direct

Name: gmd

Synonym: HH0172

Alternate gene names: 32265671

Gene position: 165492-166628 (Clockwise)

Preceding gene: 32265670

Following gene: 32265672

Centisome position: 9.2

GC content: 39.31

Gene sequence:

>1137_bases
ATGAAAAAAGCATTAATCACAGGTGTTACAGGGCAAGATGGAGCGTATTTGAGTGAATTTTTACTCAATAAAGGTTACGA
AGTTCACGGCATTAAAAGGCGTAGCTCACTTTTTAACACCGATAGAATCGATCATCTCTTTGATGGACATCATAATAAGA
AAATTCCATTTTACTTGCATTTTGGCGATATGACAGATTCTATGAATCTCACGCGTTTGATTGCAGAAGTCCAACCTGAT
GAAATCTATAATCTCGCCGCGCAAAGCCACGTAGCTGTAAGCTTTGAAACACCCGAATATACTGCAAATGCCGATGGAAT
AGGCACGCTTAGAATCTTAGAAGCAGTAAAATTCTTAAATCTTATTGACAAAACGCGCATTTATCAGGCTTCCACCTCTG
AACTCTTTGGCAAGGTGCAAGAGATTCCTCAAAGTGAGAGCACACCCTTTTATCCCCGCTCTCCTTATGCAGTGGCGAAA
ATGTATGCGTATTGGATTACGGTGAATTATAGAGAAGCCTATAATATGTTTGCGTGTAATGGAATTTTGTTTAATCACGA
AAGCCCTGTGCGAGGTGAAACCTTTGTAACACGTAAAATTACACGTGCAGCTTCTAAAATTGCACTCAATCTACAAGATA
AGCTCTATCTTGGCAATTTAGACGCTAAACGCGATTGGGGACATGCAAAGGATTATGTCAAAATGATGTGGTTAATTTTA
CAACACGAAAAGGCAGAGGATTGGGTGATTGCCACAGGGAAAACGACACAAGTTAGAGACTTTGTGCGTTTAGCATTTGC
CTATTGTGGTATCACATTAGCATTTCAAGGAAGTGGCGCAGATGAAAAAGGCGTTGTAAGTGCGTTAGATTCTGCTAAAG
CAGTAAGTCTTGGATTAAATTTAGAGCATCTTAAAATCGGACAAGAAGTTGTTGCGGTAGATAAGCGATATTTTCGCCCC
ACAGAAGTAGATTTACTCCTTGGCGACCCAAGCAAAGCTGAAAAAGAGTTAGGCTGGAATCGTGAATTTGACTTGAAAGA
TTTAGTCAATGATATGATGGAGAGCGATTTAAAACTAATGCGCAAAGATGTGTATCTCAAAGAAGGTGGATACAAAATTA
TGCGGTATTTTGAATAA

Upstream 100 bases:

>100_bases
TTCTTGCACACCACACTTGGTAAATAAGGTGTTTTGAGATTTAAGCAAACCTAATCTACAATACATTTTTTAATTTTTAT
ATTAAAATAAAAGGTTAAGA

Downstream 100 bases:

>100_bases
TGAAGATTTTAATCATATAAGGAAGCAAATGCAAAAAGATTCTAAAATCTATGTTGCAGGGCATAGGGGACTTGTGGGGA
GTGCAATTTATAGAGAACTT

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKKALITGVTGQDGAYLSEFLLNKGYEVHGIKRRSSLFNTDRIDHLFDGHHNKKIPFYLHFGDMTDSMNLTRLIAEVQPD
EIYNLAAQSHVAVSFETPEYTANADGIGTLRILEAVKFLNLIDKTRIYQASTSELFGKVQEIPQSESTPFYPRSPYAVAK
MYAYWITVNYREAYNMFACNGILFNHESPVRGETFVTRKITRAASKIALNLQDKLYLGNLDAKRDWGHAKDYVKMMWLIL
QHEKAEDWVIATGKTTQVRDFVRLAFAYCGITLAFQGSGADEKGVVSALDSAKAVSLGLNLEHLKIGQEVVAVDKRYFRP
TEVDLLLGDPSKAEKELGWNREFDLKDLVNDMMESDLKLMRKDVYLKEGGYKIMRYFE

Sequences:

>Translated_378_residues
MKKALITGVTGQDGAYLSEFLLNKGYEVHGIKRRSSLFNTDRIDHLFDGHHNKKIPFYLHFGDMTDSMNLTRLIAEVQPD
EIYNLAAQSHVAVSFETPEYTANADGIGTLRILEAVKFLNLIDKTRIYQASTSELFGKVQEIPQSESTPFYPRSPYAVAK
MYAYWITVNYREAYNMFACNGILFNHESPVRGETFVTRKITRAASKIALNLQDKLYLGNLDAKRDWGHAKDYVKMMWLIL
QHEKAEDWVIATGKTTQVRDFVRLAFAYCGITLAFQGSGADEKGVVSALDSAKAVSLGLNLEHLKIGQEVVAVDKRYFRP
TEVDLLLGDPSKAEKELGWNREFDLKDLVNDMMESDLKLMRKDVYLKEGGYKIMRYFE
>Mature_378_residues
MKKALITGVTGQDGAYLSEFLLNKGYEVHGIKRRSSLFNTDRIDHLFDGHHNKKIPFYLHFGDMTDSMNLTRLIAEVQPD
EIYNLAAQSHVAVSFETPEYTANADGIGTLRILEAVKFLNLIDKTRIYQASTSELFGKVQEIPQSESTPFYPRSPYAVAK
MYAYWITVNYREAYNMFACNGILFNHESPVRGETFVTRKITRAASKIALNLQDKLYLGNLDAKRDWGHAKDYVKMMWLIL
QHEKAEDWVIATGKTTQVRDFVRLAFAYCGITLAFQGSGADEKGVVSALDSAKAVSLGLNLEHLKIGQEVVAVDKRYFRP
TEVDLLLGDPSKAEKELGWNREFDLKDLVNDMMESDLKLMRKDVYLKEGGYKIMRYFE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=361, Percent_Identity=56.2326869806094, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1788366, Length=372, Percent_Identity=62.6344086021505, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI48994969, Length=254, Percent_Identity=25.1968503937008, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=266, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=364, Percent_Identity=59.8901098901099, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17539424, Length=364, Percent_Identity=58.7912087912088, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI133901788, Length=364, Percent_Identity=58.7912087912088, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17539422, Length=364, Percent_Identity=58.7912087912088, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI133901786, Length=364, Percent_Identity=58.7912087912088, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI133901790, Length=364, Percent_Identity=58.7912087912088, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24158427, Length=363, Percent_Identity=57.3002754820937, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43078; Mature: 43078

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGVTGQDGAYLSEFLLNKGYEVHGIKRRSSLFNTDRIDHLFDGHHNKKIPFYLH
CCCCEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHCCCCCCCCEEEEE
FGDMTDSMNLTRLIAEVQPDEIYNLAAQSHVAVSFETPEYTANADGIGTLRILEAVKFLN
ECCCCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LIDKTRIYQASTSELFGKVQEIPQSESTPFYPRSPYAVAKMYAYWITVNYREAYNMFACN
HHHHHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEEC
GILFNHESPVRGETFVTRKITRAASKIALNLQDKLYLGNLDAKRDWGHAKDYVKMMWLIL
EEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
QHEKAEDWVIATGKTTQVRDFVRLAFAYCGITLAFQGSGADEKGVVSALDSAKAVSLGLN
HCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHCCCCEEEECCC
LEHLKIGQEVVAVDKRYFRPTEVDLLLGDPSKAEKELGWNREFDLKDLVNDMMESDLKLM
HHHHHHCHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RKDVYLKEGGYKIMRYFE
HHHHEEECCCEEEEEECC
>Mature Secondary Structure
MKKALITGVTGQDGAYLSEFLLNKGYEVHGIKRRSSLFNTDRIDHLFDGHHNKKIPFYLH
CCCCEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHCCCCCCCCEEEEE
FGDMTDSMNLTRLIAEVQPDEIYNLAAQSHVAVSFETPEYTANADGIGTLRILEAVKFLN
ECCCCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LIDKTRIYQASTSELFGKVQEIPQSESTPFYPRSPYAVAKMYAYWITVNYREAYNMFACN
HHHHHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEEC
GILFNHESPVRGETFVTRKITRAASKIALNLQDKLYLGNLDAKRDWGHAKDYVKMMWLIL
EEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
QHEKAEDWVIATGKTTQVRDFVRLAFAYCGITLAFQGSGADEKGVVSALDSAKAVSLGLN
HCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHCCCCEEEECCC
LEHLKIGQEVVAVDKRYFRPTEVDLLLGDPSKAEKELGWNREFDLKDLVNDMMESDLKLM
HHHHHHCHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RKDVYLKEGGYKIMRYFE
HHHHEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]