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The map label for this gene is hyaD

Identifier: 32265558

GI number: 32265558

Start: 64327

End: 64866

Strand: Direct

Name: hyaD

Synonym: HH0059

Alternate gene names: 32265558

Gene position: 64327-64866 (Clockwise)

Preceding gene: 32265557

Following gene: 32265559

Centisome position: 3.58

GC content: 37.78

Gene sequence:

>540_bases
ATGAAAATTTTAGTGCTTGGCATTGGTAATATCCTCTTTGGTGATGAGGGTATTGGGGTGCATTTAGCCAACTTTCTTAA
ACTCAATTATCGCTTTAGCGGGGAAAATGAGGTAGAGTTTGTCGATGGTGGCACACTTGCTTCTATGCTCATTCCCCTGA
TTACTTCTTATGATAAGGTTCTTATTCTTGATTGCGTAGATGTCAATAATGCCAACATTGGTGAGGTATATTATTTTGAT
TTTGATAATGTGCCAAATATTATCACTTGGGCAGGAAGCGCACACGAAGTAGAGATGCTTCAAACATTACGTCTTACCGC
AATTAATGGTGATTTACCCCCTGTTAAAATTATTGGCATCATTCCTGAAGTCATTGGCGAAGATACAGCTTTTACTCTCT
CGCAACCTGTAAAAGAAGGTGCAAAGATTATGGAAAAGCTTGCTCTAGAATTTTTAGAAGAAAATGGTATCCACGCACAA
AAAGTTGACAATAGAGATTTACAAGAAGTCGCTAATGGTTCTTTTAGAGGCTATGAATGA

Upstream 100 bases:

>100_bases
TATTTTGCAGTATGGAATTCAATTAAATACCCAAATGGTGGTGTAGATGCTATTGTAAGCGGTATACGCTACACTGATGA
AGTAAAAGTTTAAGGCTTGT

Downstream 100 bases:

>100_bases
TTTATGATTTTAGATTCTATAATAAAAATCAAGATGAAAGCTTTTTTCTCTTTCTCCTTGCTTCAAAAGCTCAAGTCCTT
AACTTAGAAGCATTTTTTTA

Product: hydrogenase formation/expression protease HyaD/HupD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKILVLGIGNILFGDEGIGVHLANFLKLNYRFSGENEVEFVDGGTLASMLIPLITSYDKVLILDCVDVNNANIGEVYYFD
FDNVPNIITWAGSAHEVEMLQTLRLTAINGDLPPVKIIGIIPEVIGEDTAFTLSQPVKEGAKIMEKLALEFLEENGIHAQ
KVDNRDLQEVANGSFRGYE

Sequences:

>Translated_179_residues
MKILVLGIGNILFGDEGIGVHLANFLKLNYRFSGENEVEFVDGGTLASMLIPLITSYDKVLILDCVDVNNANIGEVYYFD
FDNVPNIITWAGSAHEVEMLQTLRLTAINGDLPPVKIIGIIPEVIGEDTAFTLSQPVKEGAKIMEKLALEFLEENGIHAQ
KVDNRDLQEVANGSFRGYE
>Mature_179_residues
MKILVLGIGNILFGDEGIGVHLANFLKLNYRFSGENEVEFVDGGTLASMLIPLITSYDKVLILDCVDVNNANIGEVYYFD
FDNVPNIITWAGSAHEVEMLQTLRLTAINGDLPPVKIIGIIPEVIGEDTAFTLSQPVKEGAKIMEKLALEFLEENGIHAQ
KVDNRDLQEVANGSFRGYE

Specific function: Protease Involved In The C-Terminal Processing Of Hyab, The Large Subunit Of Hydrogenase 1 (Potential). [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=140, Percent_Identity=30.7142857142857, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789367, Length=162, Percent_Identity=30.2469135802469, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 19714; Mature: 19714

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVLGIGNILFGDEGIGVHLANFLKLNYRFSGENEVEFVDGGTLASMLIPLITSYDKV
CEEEEEECCCEEECCCCCCEEEEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCEE
LILDCVDVNNANIGEVYYFDFDNVPNIITWAGSAHEVEMLQTLRLTAINGDLPPVKIIGI
EEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHEEEEECCCCCCEEEEEE
IPEVIGEDTAFTLSQPVKEGAKIMEKLALEFLEENGIHAQKVDNRDLQEVANGSFRGYE
CHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKILVLGIGNILFGDEGIGVHLANFLKLNYRFSGENEVEFVDGGTLASMLIPLITSYDKV
CEEEEEECCCEEECCCCCCEEEEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCEE
LILDCVDVNNANIGEVYYFDFDNVPNIITWAGSAHEVEMLQTLRLTAINGDLPPVKIIGI
EEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHEEEEECCCCCCEEEEEE
IPEVIGEDTAFTLSQPVKEGAKIMEKLALEFLEENGIHAQKVDNRDLQEVANGSFRGYE
CHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587288; 9639603; 14500908 [H]